Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,035,437 T→C 82.6% K282R (AAG→AGG)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,035,4370TC82.6% 268.6 / 43.8 109K282R (AAG→AGG) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (9/10);  new base C (44/46);  total (53/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

Only 100 of 110 total aligned reads displayed.

GAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGG  >  NC_000913/3035290‑3035577
                                                                                                                                                   |                                                                                                                                            
gAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCt                                                                                                                                              <  1:262913/148‑1 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTtgtg                                                                                                                                          >  2:178537/1‑148 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTtgtg                                                                                                                                          >  3:231558/1‑148 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTtgtg                                                                                                                                          >  1:322536/1‑148 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCtgtg                                                                                                                                          >  2:198197/1‑148 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCtgtg                                                                                                                                          >  8:258149/1‑148 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTAACTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCtgtg                                                                                                                                          >  7:21105/1‑148 (MQ=255)
    catcaAGGACATAAGAACGAATCTGGCTGCCCAAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAATGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTtgtg                                                                                                                                          >  2:240000/1‑148 (MQ=255)
      tcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCt                                                                                                                                        >  2:278246/1‑148 (MQ=255)
      tcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCAGCTTCATGGCCTGATCTTTGTTCTTGTGCt                                                                                                                                        >  1:253593/1‑148 (MQ=255)
       caAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTg                                                                                                                                       >  6:336440/1‑148 (MQ=255)
         aGGACATAAGAACGACTCTGGCTGCCCCAGCCGATGTCGGACTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCACTTCATAAAGCATCGCTTTCATCTGCTTCATGGCCTGAGCTTTGTTCCTGTGCTggg                                                                                                                                     <  8:21105/148‑1 (MQ=255)
                aaGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTc                                                                                                                              >  3:93680/1‑148 (MQ=255)
                 aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTc                                                                                                                              <  3:96644/147‑1 (MQ=255)
                  gAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGt                                                                                                                            <  5:336440/148‑1 (MQ=255)
                  gAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCATTGTTCCTGTGCTGGGAACGGTCGt                                                                                                                            <  5:377801/148‑1 (MQ=255)
                    aCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTc                                                                                                                          <  4:231558/148‑1 (MQ=255)
                    aCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTc                                                                                                                          >  3:192260/1‑148 (MQ=255)
                    aCGAATCTGGCTGCCCCAGCCGATGTCGGAGTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTc                                                                                                                          <  2:322536/148‑1 (MQ=255)
                      gAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGACCGGTCGTTCTg                                                                                                                        <  3:159897/148‑1 (MQ=255)
                        aTCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGc                                                                                                                      <  7:258149/148‑1 (MQ=255)
                         tCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGtt                                                                                                                           <  2:337926/142‑1 (MQ=255)
                         tCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGtt                                                                                                                           >  1:337926/1‑142 (MQ=255)
                                    cAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGa                                                                                                          <  1:178537/148‑1 (MQ=255)
                                     aGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGAt                                                                                                         >  6:112490/1‑148 (MQ=255)
                                             tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTc                                                                                                  <  7:213539/147‑1 (MQ=255)
                                                 aTTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGc                                                                                                                      >  5:201038/1‑123 (MQ=255)
                                                    tGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtgt                                                                                           <  7:83927/147‑1 (MQ=255)
                                                      ttATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                         <  6:374776/147‑1 (MQ=255)
                                                         tCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTa                                                                                      <  1:240000/147‑1 (MQ=255)
                                                         tCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTa                                                                                      <  1:198197/147‑1 (MQ=255)
                                                           ttCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATa                                                                                   >  5:191396/1‑148 (MQ=255)
                                                             ccctCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAAt                                                                                    <  6:254163/142‑1 (MQ=255)
                                                                        ttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtgt                                                                                           <  4:64474/127‑1 (MQ=255)
                                                                        ttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtgt                                                                                           >  3:64474/1‑127 (MQ=255)
                                                                        ttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAAt                                                                                    <  2:210977/134‑1 (MQ=255)
                                                                        ttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAAt                                                                                    >  1:210977/1‑134 (MQ=255)
                                                                             ggCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCgg                                                                   <  8:372628/146‑1 (MQ=255)
                                                                             ggCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGt                                                                  <  7:274157/147‑1 (MQ=255)
                                                                                  ttttCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTc                                                                     >  1:292483/1‑139 (MQ=255)
                                                                                  ttttCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTc                                                                     <  2:292483/139‑1 (MQ=255)
                                                                                  ttttCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGt                                                             >  4:237364/1‑147 (MQ=255)
                                                                                   tttCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGa                                                                                              <  5:364647/113‑1 (MQ=255)
                                                                                   tttCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGa                                                                                              >  6:364647/1‑113 (MQ=255)
                                                                                    ttcttcTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTa                                                           <  6:191396/147‑1 (MQ=255)
                                                                                     tcttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGtt                                                                                                                           >  8:388557/1‑82 (MQ=255)
                                                                                     tcttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGtt                                                                                                                           <  7:388557/82‑1 (MQ=255)
                                                                                      cttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTa                                                                                      <  3:162107/118‑1 (MQ=255)
                                                                                      cttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTa                                                                                      >  4:162107/1‑118 (MQ=255)
                                                                                      cttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAAc                                                         <  1:16030/147‑1 (MQ=255)
                                                                                         ctGCATCTCCAGAGCATAAAGCTTCGCTTTCATCTGCATCATGGCCTGATCTTTGTTCCTGTGCTGGGCACGGTCGTTCTGGCACTGGGGCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTg                                                      <  4:192260/147‑1 (MQ=255)
                                                                                            cATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTg                                                   <  5:112490/147‑1 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGc                                                                                                                      >  3:176139/1‑77 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCgg                                                                   >  8:55009/1‑128 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCgg                                                                   <  7:55009/128‑1 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTa                                                           >  4:92627/1‑136 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTa                                                           <  3:92627/136‑1 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGa                                                  >  6:95588/1‑145 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGa                                                  <  5:95588/145‑1 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccg                                               >  7:144241/1‑148 (MQ=255)
                                                                                               ctcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccg                                               >  4:56620/1‑148 (MQ=255)
                                                                                                tcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgc                                              >  2:119696/1‑148 (MQ=255)
                                                                                                tcCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgc                                              >  2:175113/1‑148 (MQ=255)
                                                                                                  cAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgccc                                            >  7:127304/1‑148 (MQ=255)
                                                                                                   aGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcccg                                           >  3:70329/1‑148 (MQ=255)
                                                                                                      tCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtg                                                                                            >  5:26565/1‑96 (MQ=255)
                                                                                                      tCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtg                                                                                            <  6:26565/96‑1 (MQ=255)
                                                                                                          aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTa                                                                                      >  8:6577/1‑98 (MQ=255)
                                                                                                          aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGa                                    >  8:23874/1‑148 (MQ=255)
                                                                                                               ttCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGc                               >  2:16932/1‑148 (MQ=255)
                                                                                                                 cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCg                                                                    >  5:320004/1‑109 (MQ=255)
                                                                                                                 cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCg                                                                    <  6:320004/109‑1 (MQ=255)
                                                                                                                 cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTg                                                      <  3:19125/123‑1 (MQ=255)
                                                                                                                 cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTg                                                      >  4:19125/1‑123 (MQ=255)
                                                                                                                 cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGc                               <  4:70329/146‑1 (MQ=255)
                                                                                                                      tCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAAc                        >  6:196567/1‑148 (MQ=255)
                                                                                                                      tCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAAc                        >  6:201768/1‑148 (MQ=255)
                                                                                                                      tCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAAc                        >  5:44152/1‑148 (MQ=255)
                                                                                                                       cATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACg                       >  5:100101/1‑148 (MQ=255)
                                                                                                                         tCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGt                      >  3:287493/1‑147 (MQ=255)
                                                                                                                          cttcTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaa                          >  3:258541/4‑142 (MQ=255)
                                                                                                                          cttcTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaa                          <  4:258541/139‑1 (MQ=255)
                                                                                                                          cTGCTTCATGGCCTGATCTTTGTTCCTGTGTTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                     >  4:271195/1‑147 (MQ=255)
                                                                                                                          cTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                         >  8:355472/1‑79 (MQ=255)
                                                                                                                          cTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                         <  7:355472/79‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                     <  1:16932/144‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                     <  6:44152/144‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                     <  5:201768/144‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                     <  5:196567/144‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCa                    <  6:100101/145‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCa                    <  1:175113/145‑1 (MQ=255)
                                                                                                                             cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGGCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                     <  3:56620/144‑1 (MQ=255)
                                                                                                                                   ggCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTaa                                                          >  4:94379/1‑101 (MQ=255)
                                                                                                                                   ggCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTaa                                                          <  3:94379/101‑1 (MQ=255)
                                                                                                                                   ggCCTGATCTTTGTTCCAGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTaa                                                          >  6:357393/1‑101 (MQ=255)
                                                                                                                                    gCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAt          <  8:144241/148‑1 (MQ=255)
                                                                                                                                         aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccgcc     >  1:180474/1‑148 (MQ=255)
                                                                                                                                          tCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAAt                                                                                    >  6:378296/1‑68 (MQ=255)
                                                                                                                                          tCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAAt                                                                                    <  5:378296/68‑1 (MQ=255)
                                                                                                                                          tCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCCCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccgccg    >  6:144805/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGG  >  NC_000913/3035290‑3035577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: