Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,484,760 A→G 6.0% K91E (AAA→GAA)  prkB → putative phosphoribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,484,7600AG 6.0% 209.1 / 3.7 67K91E (AAA→GAA) prkBputative phosphoribulokinase
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base G (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGTAGT  >  NC_000913/3484614‑3484899
                                                                                                                                                  |                                                                                                                                           
aCAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCa                                                                                                                                             >  8:485/1‑147 (MQ=255)
aCAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCa                                                                                                                                             >  7:328754/1‑147 (MQ=255)
      ttCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGa                                                                                                                                       <  1:148827/147‑1 (MQ=255)
              tACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGc                                                                                                                               >  4:89666/1‑147 (MQ=255)
              tACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGc                                                                                                                               >  7:48020/1‑147 (MQ=255)
                      cccgGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTg                                                                                                                      <  4:3899/148‑1 (MQ=255)
                      cccgGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTg                                                                                                                      <  3:89666/148‑1 (MQ=255)
                         ggAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGCGCGGCAAAGGGAAATCTCGCAAATATCTGCa                                                                                                                    >  5:83946/1‑147 (MQ=255)
                          gAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATa                                                                                                                  <  7:485/148‑1 (MQ=255)
                           aaaTGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCaaa                                                                                                                            >  1:53457/1‑137 (MQ=255)
                           aaaTGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCaaa                                                                                                                            <  2:53457/137‑1 (MQ=255)
                                    tGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTAcgacga                                                                                                        <  5:348003/148‑1 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCGAAGGGAAATCTCGCAAATATCTGc                                                                                                                     <  8:378245/134‑1 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCGAAGGGAAATCTCGCAAATATCTGc                                                                                                                     >  3:336613/1‑134 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCGAAGGGAAATCTCGCAAATATCTGc                                                                                                                     >  7:378245/1‑134 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCGAAGGGAAATCTCGCAAATATCTGc                                                                                                                     <  4:336613/134‑1 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTg                                                                                                                      >  7:22267/1‑133 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTg                                                                                                                      <  8:22267/133‑1 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTacg                                                                                                            <  2:45318/143‑1 (MQ=255)
                                     ggCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTacg                                                                                                            >  1:45318/1‑143 (MQ=255)
                                      gCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAg                                                                                                      >  6:357686/1‑148 (MQ=255)
                                                          cGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTa                                                                                  <  8:328754/148‑1 (MQ=255)
                                                               ggcggcATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCggg                                                                             >  2:255008/1‑148 (MQ=255)
                                                                       atCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCt                                                                                                               >  8:103376/1‑106 (MQ=255)
                                                                       atCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCt                                                                                                               <  7:103376/106‑1 (MQ=255)
                                                                       atCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACAGGGGACATTCa                                                                     >  4:200766/1‑148 (MQ=255)
                                                                        tCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTa                                                                                                 <  5:60209/119‑1 (MQ=255)
                                                                        tCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTa                                                                                                 >  6:60209/1‑119 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTgg                                                                                           >  8:94546/1‑124 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTgg                                                                                           >  8:89139/1‑124 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTgg                                                                                           <  7:89139/124‑1 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTgg                                                                                           <  7:94546/124‑1 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCg                                                                               <  7:236493/136‑1 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCg                                                                               >  8:236493/1‑136 (MQ=255)
                                                                         cAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCAcc                                                                   >  4:122136/1‑148 (MQ=255)
                                                                          aGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGTAATCAGGTACCGGGGACATTCAccc                                                                  <  4:343386/148‑1 (MQ=255)
                                                                           gCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTa                                                                                  <  5:164094/131‑1 (MQ=255)
                                                                           gCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTa                                                                                  >  6:164094/1‑131 (MQ=255)
                                                                           gCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCgggg                                                                            >  6:270469/1‑137 (MQ=255)
                                                                           gCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCgggg                                                                            <  5:270469/137‑1 (MQ=255)
                                                                                 tCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAata                                                                                                                          <  6:208697/85‑1 (MQ=255)
                                                                                 tCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAata                                                                                                                          >  5:208697/1‑85 (MQ=255)
                                                                                 tCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGCCATTCACCCCCTGGc                                                            >  5:21004/1‑147 (MQ=255)
                                                                                 tCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGc                                                            >  8:201603/1‑147 (MQ=255)
                                                                                     ccccGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCt                                                       <  3:122136/148‑1 (MQ=255)
                                                                                     ccccGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCt                                                       <  5:357686/148‑1 (MQ=255)
                                                                                         gAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTAc                                                   >  2:28690/1‑148 (MQ=255)
                                                                                          aGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGTCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGACGCCGTACCGTGGAATCAGGTTCCGGGGACCTTCACCCCCTGGCAACCTTTAcc                                                  >  8:387506/1‑148 (MQ=255)
                                                                                             cTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGa                                               <  5:146529/148‑1 (MQ=255)
                                                                                              tAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGaa                                              >  7:386354/1‑148 (MQ=255)
                                                                                                 cGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAAccc                                           <  6:83946/148‑1 (MQ=255)
                                                                                                 cGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAAccc                                           <  8:386354/148‑1 (MQ=255)
                                                                                                        ggCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGAt                                     <  3:200766/147‑1 (MQ=255)
                                                                                                        ggCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGAATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGAt                                     <  7:201603/147‑1 (MQ=255)
                                                                                                           ctgctgGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTAc                                 >  7:274127/1‑148 (MQ=255)
                                                                                                             gctgGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGCCATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTg                               >  5:294820/1‑148 (MQ=255)
                                                                                                              ctgGAACAAACCGTCCTTGACTACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAATCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGt                              <  6:21004/148‑1 (MQ=255)
                                                                                                               tgGAACAAACCTTCATTGAATACGGTCAGAGCTGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATc                                                                                       <  3:350813/90‑1 (MQ=255)
                                                                                                               tgGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATc                                                                                       >  4:350813/1‑90 (MQ=255)
                                                                                                                 gAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGtttt                           <  1:255008/148‑1 (MQ=255)
                                                                                                                     aaaCCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGa                       <  1:28690/148‑1 (MQ=255)
                                                                                                                           tCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGaa                                              <  1:183035/119‑1 (MQ=255)
                                                                                                                           tCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGaa                                              >  2:183035/1‑119 (MQ=255)
                                                                                                                               tGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTacac             >  8:387362/1‑148 (MQ=255)
                                                                                                                                  aTACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGtt                  >  1:264893/1‑140 (MQ=255)
                                                                                                                                  aTACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGtt                  <  2:264893/140‑1 (MQ=255)
                                                                                                                                      ggTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGgcggcg      <  8:48020/148‑1 (MQ=255)
                                                                                                                                          agagCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGTAg   <  7:387362/147‑1 (MQ=255)
                                                                                                                                           gagCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTGCTGTTTTATGAAGGTTTACACGGCGGCGTAGt  >  1:326356/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
ACAGTTTTCACCGTTACACCCGCCCGGAAATGGACATGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAGGCTAACGACTTCGGCCTGCTGGAACAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCGTGGAATCAGGTACCGGGGACATTCACCCCCTGGCAACCTTTACCGGAACCCACTGATGTACTGTTTTATGAAGGTTTACACGGCGGCGTAGT  >  NC_000913/3484614‑3484899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑