Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,984,297 T→C 5.1% E258G (GAG→GGG)  araG ← L‑arabinose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,984,2970TC 5.1% 249.4 / 2.4 78E258G (GAG→GGG) araGL‑arabinose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (40/34);  new base C (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGA  >  NC_000913/1984152‑1984444
                                                                                                                                                 |                                                                                                                                                   
cGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCg                                                                                                                                                   <  4:48353/148‑1 (MQ=255)
cGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCACGCCCCTACCAGACCAAACATCCCAACAATTGCCCCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGc                                                                                                                                                  <  6:272394/149‑1 (MQ=255)
  tCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCa                                                                                                                                                <  7:355532/149‑1 (MQ=255)
   cccGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTACTCGCCa                                                                                                                                                >  5:266316/1‑148 (MQ=255)
      gCCAAACATGACTTTAATTAATTCTCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACAACTGCTAACCACCAGACTTATTGGCCTACGCACGCCTGGTGGTTTCACAGCATAAAGACGAAAGCGCTCCTCGCCATAAc                                                                                                                                            >  8:437141/1‑149 (MQ=255)
        cAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTa                                                                                                                                          <  6:178559/149‑1 (MQ=255)
        cAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCCCGCCATAACTa                                                                                                                                          >  5:72469/1‑149 (MQ=255)
        cAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCCCGCCATAACTa                                                                                                                                          >  7:119740/1‑149 (MQ=255)
         aaaCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAc                                                                                                                                         <  1:11081/149‑1 (MQ=255)
         aaaCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAc                                                                                                                                         <  4:400483/149‑1 (MQ=255)
              tGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGt                                                                                                                                    <  5:75986/149‑1 (MQ=255)
                    tCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAg                                                                                                                              >  5:203826/1‑149 (MQ=255)
                             cGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGata                                                                                                                     >  2:94057/1‑149 (MQ=255)
                              gCTACGGCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGatat                                                                                                                    >  5:35935/1‑149 (MQ=255)
                              gCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGata                                                                                                                     <  6:203826/148‑1 (MQ=255)
                               cTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATc                                                                                                                   >  6:281841/1‑149 (MQ=255)
                                  cgccccgcccCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCg                                                                                                                  <  5:281841/147‑1 (MQ=255)
                                   gccccgccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGcc                                                                                                                >  2:41196/1‑148 (MQ=255)
                                      ccgccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATg                                                                                                            >  5:229375/1‑149 (MQ=255)
                                        gccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCGGGTGCTTTCCCAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTc                                                                                                          <  3:108939/149‑1 (MQ=255)
                                           ccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGc                                                                                                                                                  >  1:60689/1‑106 (MQ=255)
                                           ccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGc                                                                                                                                                  <  2:60689/106‑1 (MQ=255)
                                           ccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTcgcg                                                                                                       >  5:35186/1‑149 (MQ=255)
                                               ccAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTTTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGccc                                                                                                    <  6:266316/148‑1 (MQ=255)
                                                     cAAACAGCCCAACAATTTTACCACTGCGACCCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCCCAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATc                                                                                             >  1:390743/1‑149 (MQ=255)
                                                     cAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATc                                                                                             >  7:40084/1‑149 (MQ=255)
                                                       aaCAGCCCAACAATTTCACCACTGCGACCCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTGTCACAGCATCAAGCCGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGc                                                                                           >  2:183401/1‑149 (MQ=255)
                                                         gttctcaactaTATCACCACTGGGAACCGCCTGACTTATTGGCGTACGCACGCCTGGTGCTTTCACTGGATCAAGACGTAGGCGCTCCTCTCCATAACTACGCGGTTGCCAGCCGTTTATATCGCCAATGTCGCGCCCGACCTTCGCCt                                                                                         <  6:19559/144‑1 (MQ=255)
                                                         cAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCt                                                                                         <  6:222104/149‑1 (MQ=255)
                                                         cAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCCCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCt                                                                                         <  6:72469/149‑1 (MQ=255)
                                                         cAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCCCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCt                                                                                         <  8:119740/149‑1 (MQ=255)
                                                           gCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGc                                                                                       >  4:105989/1‑149 (MQ=255)
                                                            cccAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCa                                                                                      >  1:160551/1‑149 (MQ=255)
                                                              caacaaTTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCAc                                                                                     >  8:317216/1‑148 (MQ=255)
                                                               aacaaTTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACTCATGGTGCTTTCACAGCATCAACACGTAGGCGCTCCTCGCCATAACTACCCGGTTGCCAGCCGTAGATATAGCCAATGTCGCGCCCGACAATCGCCtcagaac                                                                                   >  5:19559/1‑143 (MQ=255)
                                                                      tCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGcgtc                                                                            >  4:392280/1‑149 (MQ=255)
                                                                          cacTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTgg                                                                        >  3:368449/1‑149 (MQ=255)
                                                                           acTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGt                                                                       <  8:40084/149‑1 (MQ=255)
                                                                             tGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAgcgc                                                                               >  4:379867/1‑139 (MQ=255)
                                                                             tGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAgcgc                                                                               <  3:379867/139‑1 (MQ=255)
                                                                             tGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTc                                                                      >  1:29927/1‑148 (MQ=255)
                                                                                gAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAAcc                                                                  <  8:99734/149‑1 (MQ=255)
                                                                                       cAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCACGTCGTGGTCAACCTGCTGCa                                                           >  6:92177/1‑149 (MQ=255)
                                                                                        aGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCa                                                           >  3:56982/1‑148 (MQ=255)
                                                                                                  gCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCCTCGCCTGCACCAGCGCGTCGTGGACAACCTGCTGCATATCGGTAAAg                                                >  4:276045/1‑149 (MQ=255)
                                                                                                  gCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTg                                                                         >  8:166030/1‑124 (MQ=255)
                                                                                                  gCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTg                                                                         <  7:166030/124‑1 (MQ=255)
                                                                                                  gCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAg                                                >  8:142789/1‑149 (MQ=255)
                                                                                                     tACGCACGCCTGGTGCTTTCACAGCATCAAGCCGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGtt                                             <  6:35935/149‑1 (MQ=255)
                                                                                                     tACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGtt                                             <  6:35186/149‑1 (MQ=255)
                                                                                                       cgcacgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGtttt                                           <  1:41196/149‑1 (MQ=255)
                                                                                                       cgcacgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGttt                                            <  1:94057/148‑1 (MQ=255)
                                                                                                         cacgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGa                                         <  3:392280/149‑1 (MQ=255)
                                                                                                         cacgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGa                                         <  7:317216/149‑1 (MQ=255)
                                                                                                         cacgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGa                                         <  3:105989/149‑1 (MQ=255)
                                                                                                         cacgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGa                                         <  6:229375/149‑1 (MQ=255)
                                                                                                          acgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTg                                                                         >  3:4524/1‑116 (MQ=255)
                                                                                                          acgcCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTg                                                                         <  4:4524/116‑1 (MQ=255)
                                                                                                                   gCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTc                                >  7:303949/1‑148 (MQ=255)
                                                                                                                    cTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCa                              >  5:114838/1‑149 (MQ=255)
                                                                                                                     tttCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCAt                             >  2:38112/1‑149 (MQ=255)
                                                                                                                       tCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCt                           >  2:337233/1‑149 (MQ=255)
                                                                                                                         acaGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCttt                         <  7:142789/149‑1 (MQ=255)
                                                                                                                         acaGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCtt                          >  7:247871/1‑148 (MQ=255)
                                                                                                                           aGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTaa                       <  1:337233/149‑1 (MQ=255)
                                                                                                                             cATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCTTCTTTa                        >  3:204131/1‑146 (MQ=255)
                                                                                                                             cATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAg                     <  2:390743/149‑1 (MQ=255)
                                                                                                                              aTCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGa                    >  4:187957/1‑149 (MQ=255)
                                                                                                                               tCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGAc                   <  8:247871/149‑1 (MQ=255)
                                                                                                                                cAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGa                    >  7:18054/1‑147 (MQ=255)
                                                                                                                                 aaGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAg                 <  4:368449/149‑1 (MQ=255)
                                                                                                                                  aGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAg                 >  1:274845/1‑148 (MQ=255)
                                                                                                                                  aGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAg                 >  5:317297/1‑148 (MQ=255)
                                                                                                                                    aCGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTaa              >  7:106603/1‑149 (MQ=255)
                                                                                                                                        aGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCCGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATgg           >  8:430518/1‑148 (MQ=255)
                                                                                                                                         ggCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGc          <  8:303949/148‑1 (MQ=255)
                                                                                                                                          gcgcTCCTCGCCATAACTATGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTaaa                      <  1:283378/135‑1 (MQ=255)
                                                                                                                                          gcgcTCCTCGCCATAACTATGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTaaa                      >  2:283378/1‑135 (MQ=255)
                                                                                                                                              tcctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTaa              <  6:278190/139‑1 (MQ=255)
                                                                                                                                              tcctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCt    >  7:87638/1‑149 (MQ=255)
                                                                                                                                                ctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCTATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACa                  >  5:383243/1‑133 (MQ=255)
                                                                                                                                                ctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCTATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACa                  <  6:383243/133‑1 (MQ=255)
                                                                                                                                                ctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCt    >  7:322664/1‑147 (MQ=255)
                                                                                                                                                ctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGa  <  8:322664/149‑1 (MQ=255)
                                                                                                                                                ctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGa  <  8:18054/149‑1 (MQ=255)
                                                                                                                                                ctcGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGa  <  6:114838/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGA  >  NC_000913/1984152‑1984444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: