Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,389,029	T→C	5.5%	M132T (ATG→ACG)	yfbO →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,389,029	0	T	C	5.5%	287.1 / 3.9	91	M132T (ATG→ACG)	yfbO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base C (3/2);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCGGGGCC  >  NC_000913/2388883‑2389177                                                                                                                                                   |                                                                                                                                                     gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGc                                                                                                                                                    <  5:370482/149‑1 (MQ=255) gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCGGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTAt                                                                                                                                                      <  5:291256/147‑1 (MQ=255) gCCCTTTAGTGAAAGTATTTTAGTTATCACAACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGAGGGGTTGAAGTCCCTGTGGGTATg                                                                                                                                                     >  4:319803/1‑148 (MQ=255)   ccTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAt                                                                                                                                                  <  8:4096/149‑1 (MQ=255)          tGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTa                                                                                                                                           >  4:91869/1‑149 (MQ=255)          tGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGCCCCTGTGGGTATGCATCCGGTTa                                                                                                                                           >  6:157729/1‑149 (MQ=255)            aaaGTAATTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACt                                                                                                                                         <  3:28030/149‑1 (MQ=255)             aaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCgg                                                                                                                                              <  8:127503/143‑1 (MQ=255)             aaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCgg                                                                                                                                              >  7:127503/1‑143 (MQ=255)             aaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACtt                                                                                                                                        >  4:73170/1‑149 (MQ=255)             aaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACtt                                                                                                                                        >  4:206662/1‑149 (MQ=255)                 aTTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACttgttg                                                                                                                                    >  6:419162/1‑149 (MQ=255)                  ttttAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTtgg                                                                                                                                   >  3:363689/1‑149 (MQ=255)                     tAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTtggtgg                                                                                                                                >  7:374203/1‑149 (MQ=255)                     tAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTtggtg                                                                                                                                 <  7:367190/148‑1 (MQ=255)                       tttATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGGTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGa                                                                                                                              <  5:238143/148‑1 (MQ=255)                          aTCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGAtt                                                                                                                            >  6:68290/1‑148 (MQ=255)                           tCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTa                                                                                                                           >  3:61051/1‑148 (MQ=255)                              ccacTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTaaaa                                                                                                                        >  8:320559/1‑148 (MQ=255)                               cacTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACa                                                                                                                      <  3:91869/149‑1 (MQ=255)                                 cTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACAtt                                                                                                                    >  3:311204/1‑149 (MQ=255)                                 cTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACAtt                                                                                                                    <  7:320559/149‑1 (MQ=255)                                  tGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACAtt                                                                                                                    >  5:134633/1‑148 (MQ=255)                                    cTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGTTGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACAttt                                                                                                                   <  8:181211/147‑1 (MQ=255)                                     ttCACCAGAAGAAGTTCAGAGCTGCTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATTGTCAGAAGATACGGAGCATGGATGAGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTtata                                                                                                                >  7:181834/1‑149 (MQ=255)                                       cACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTtatat                                                                                                               <  5:419162/148‑1 (MQ=255)                                       cACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTtata                                                                                                                <  3:41513/147‑1 (MQ=255)                                           agaagaAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATaaa                                                                                                            >  6:36479/1‑147 (MQ=255)                                            gaagaaGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATaaa                                                                                                            >  2:150341/1‑146 (MQ=255)                                             aagaagTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGa                                                                                                        <  2:381603/149‑1 (MQ=255)                                                aagTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATaa                                                                                                     <  5:68290/149‑1 (MQ=255)                                                  gTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGGAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGc                                                                                                   <  3:296039/149‑1 (MQ=255)                                                   ttCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCg                                                                                                  >  6:320838/1‑149 (MQ=255)                                                   ttCAGAGCTGGTTTGTAGAAGAAATTGCCCCCGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCg                                                                                                  >  3:386577/1‑149 (MQ=255)                                                     cAGAGCTGGTTTGTAGAAGAAATTGCCCCTGAAGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGa                                                                                                >  4:333273/1‑149 (MQ=255)                                                        agCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGt                                                                                             <  2:86099/149‑1 (MQ=255)                                                         gCTGGTTTGTAGAAGAAATTGCCCCTGAAGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGtt                                                                                            <  3:333273/149‑1 (MQ=255)                                                             gTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTa                                                                                          <  7:270889/147‑1 (MQ=255)                                                             gTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTAc                                                                                         <  4:363689/148‑1 (MQ=255)                                                             gTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACa                                                                                        >  5:62027/1‑149 (MQ=255)                                                              tttGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTACGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACa                                                                                        >  3:3892/1‑148 (MQ=255)                                                                  tAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTACGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAAt                                                                                   >  8:286258/1‑149 (MQ=255)                                                                  tAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTACGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAAt                                                                                   >  8:279538/1‑149 (MQ=255)                                                                   agaagaAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTAt                                                                                                                                                      >  6:311360/1‑81 (MQ=255)                                                                   agaagaAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTAt                                                                                                                                                      <  5:311360/81‑1 (MQ=255)                                                                    gaagaaATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAAttt                                                                                 >  7:316430/1‑149 (MQ=255)                                                                    gaagaaATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAAttt                                                                                 >  2:144180/1‑149 (MQ=255)                                                                     aagaaATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAAtttt                                                                                >  2:225732/1‑149 (MQ=255)                                                                       gaaATTGCCCCTGATGAGTGCTGGGAAGGATTGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTa                                                                              <  4:61051/149‑1 (MQ=255)                                                                        aaaTTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTAt                                                                             <  2:69080/149‑1 (MQ=255)                                                                                ccTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAgg                                                                      >  8:190128/1‑148 (MQ=255)                                                                                  tgatgaGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGaa                                                                    >  1:392383/1‑148 (MQ=255)                                                                                         tGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACAtgatga                                                            <  3:73170/149‑1 (MQ=255)                                                                                           cTGTGAAGGATGGTCAGAAGATTCGGCGCATGGATGTGTTGAAGTCCCTTTGTGTATGCATCCGGTTACTTGTTGGTGTTATTAAAACATTTATATAAACAGATAAGCGGATGTTTTCATAAATTTTTATGTGAAGGAACATGATGaaa                                                          <  8:181834/149‑1 (MQ=255)                                                                                           cTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGaa                                                           <  1:225732/148‑1 (MQ=255)                                                                                            tGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAAc                                                         >  3:73195/1‑149 (MQ=255)                                                                                            tGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGGTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGCTGGTGGGATTAAAACATTTATATAAACAGATAAGAGGATGTTTACATAAATTTTTATTTTAAGGAACATTATGAAAc                                                         >  7:378747/1‑149 (MQ=255)                                                                                             gggAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGTATGttt                                                                                           >  4:191656/1‑114 (MQ=255)                                                                                             gggAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGttt                                                                                           <  3:191656/114‑1 (MQ=255)                                                                                             gggAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACt                                                        >  8:209167/1‑149 (MQ=255)                                                                                              ggAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTTTGCATCCGGATACTTGATGGTGCGATTAAAACATTAATATAAACAGATAAGCGGATGt                                                                                             >  4:16776/1‑111 (MQ=255)                                                                                               gAAGGATGGTCAGAAGATACGGAGCATGGATGGTTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTa                                                      <  3:206662/149‑1 (MQ=255)                                                                                                aaGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTAt                                                     <  1:150341/149‑1 (MQ=255)                                                                                                aaGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTAt                                                     <  5:320838/149‑1 (MQ=255)                                                                                                       gTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGc                                              <  8:374203/149‑1 (MQ=255)                                                                                                        tCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTACTCg                                               >  7:356164/1‑147 (MQ=255)                                                                                                          agaagaTACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCag                                            >  6:243419/1‑148 (MQ=255)                                                                                                          agaagaTACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCag                                            >  6:182703/1‑148 (MQ=255)                                                                                                           gaagaTACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCagta                                          <  5:243419/149‑1 (MQ=255)                                                                                                           gaagaTACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCag                                            >  2:290714/1‑147 (MQ=255)                                                                                                                     aGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTc                                <  6:134633/149‑1 (MQ=255)                                                                                                                      gCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCa                               <  2:392383/149‑1 (MQ=255)                                                                                                                            atggGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTgg                          >  4:222121/1‑148 (MQ=255)                                                                                                                                gTTTAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGata                                    <  3:129750/134‑1 (MQ=255)                                                                                                                                gTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGata                                    >  4:129750/1‑134 (MQ=255)                                                                                                                                gTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCAtt                      >  8:162574/1‑148 (MQ=255)                                                                                                                                gTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCAtt                      >  7:324739/1‑148 (MQ=255)                                                                                                                                gTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCAtt                      >  8:297158/1‑148 (MQ=255)                                                                                                                                 ttGAAGCCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTAAATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGa                    <  5:157729/149‑1 (MQ=255)                                                                                                                                   gAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGAt                   <  5:36479/148‑1 (MQ=255)                                                                                                                                   gAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCGGGCATTGAt                   <  4:386577/148‑1 (MQ=255)                                                                                                                                      gTCCCTGTGGGTATGCTTCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGaa               >  6:211070/1‑149 (MQ=255)                                                                                                                                      gTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGaa               <  1:144180/149‑1 (MQ=255)                                                                                                                                       tCCCTGTGGGTACGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAAt              <  7:286258/149‑1 (MQ=255)                                                                                                                                       tCCCTGTGGGTACGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAAt              <  7:279538/149‑1 (MQ=255)                                                                                                                                                gTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGat                                     >  7:37022/1‑117 (MQ=255)                                                                                                                                                gTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGat                                     <  8:37022/117‑1 (MQ=255)                                                                                                                                                gTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCggg     >  5:103425/1‑149 (MQ=255)                                                                                                                                                gTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCggg     <  5:182703/149‑1 (MQ=255)                                                                                                                                                gTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCggg     <  7:297158/149‑1 (MQ=255)                                                                                                                                                 tATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGAGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCgggg    >  2:163413/1‑149 (MQ=255)                                                                                                                                                  aTGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCAtt                      <  5:203387/130‑1 (MQ=255)                                                                                                                                                  aTGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCAtt                      >  6:203387/1‑130 (MQ=255)                                                                                                                                                  aTGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCGGGGc   >  7:184468/1‑149 (MQ=255)                                                                                                                                                   tGCATCCGGTTACTTGTTGGTGGAATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCGGGGcc  >  3:198821/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACATAAATTTTTATGTGAAGGAACATGATGAAACTTATTCCTCGCAGTAGTGATATTTCACCTGGCATTGATGGAATTTGCCCGGGGCC  >  NC_000913/2388883‑2389177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑