Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,571,486 T→A 5.1% D89V (GAT→GTT)  eutE ← aldehyde oxidoreductase, ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,571,4860TA 5.1% 253.0 / 3.4 78D89V (GAT→GTT) eutEaldehyde oxidoreductase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base T (36/38);  new base A (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

GGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACTTTGGCTG  >  NC_000913/2571339‑2571627
                                                                                                                                                   |                                                                                                                                             
gggTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATc                                                                                                                                              <  1:302732/149‑1 (MQ=255)
 ggTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCt                                                                                                                                             <  1:110216/149‑1 (MQ=255)
                tCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATg                                                                                                                              >  5:318134/1‑149 (MQ=255)
                   ccGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCaa                                                                                                                                         >  2:409049/1‑135 (MQ=255)
                   ccGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCaa                                                                                                                                         <  1:409049/135‑1 (MQ=255)
                     gAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAAGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGt                                                                                                                         <  6:344724/149‑1 (MQ=255)
                          caccacGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCAc                                                                                                                    <  1:372631/149‑1 (MQ=255)
                           accacGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACt                                                                                                                   >  3:63943/1‑149 (MQ=255)
                           accacGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACt                                                                                                                   >  1:433521/1‑149 (MQ=255)
                               cGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCtt                                                                                                                                            >  8:308201/1‑120 (MQ=255)
                               cGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCtt                                                                                                                                            <  7:308201/120‑1 (MQ=255)
                               cGCCCCAGGGTGCGTTTTCAATTAGGCTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACg                                                                                                               <  8:148037/149‑1 (MQ=255)
                                  cccAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTAt                                                                                                                                               <  6:283398/114‑1 (MQ=255)
                                  cccAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTAt                                                                                                                                               >  5:283398/1‑114 (MQ=255)
                                  cccAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGc                                                                                                             >  7:371470/1‑148 (MQ=255)
                                  cccAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGc                                                                                                             >  8:250046/1‑148 (MQ=255)
                                      ggttGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGtt                                                                                                        <  6:226842/146‑1 (MQ=255)
                                         tGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCg                                                                                                     >  3:56925/1‑149 (MQ=255)
                                                   aTTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTAACTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATctct                                                                                           >  4:370279/1‑149 (MQ=255)
                                                   aTTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTAACTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATctct                                                                                           >  4:376184/1‑149 (MQ=255)
                                                    ttAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTg                                                                                          <  5:32491/149‑1 (MQ=255)
                                                       gggTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCg                                                                                       >  5:175297/1‑149 (MQ=255)
                                                              gCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTc                                                                                                                                           >  1:307122/1‑90 (MQ=255)
                                                              gCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTc                                                                                                                                           <  2:307122/90‑1 (MQ=255)
                                                              gCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAAATTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGttttt                                                                                >  2:412162/1‑149 (MQ=255)
                                                                cGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCg                                                                              >  5:213898/1‑149 (MQ=255)
                                                                 gTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTAACTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGc                                                                             <  3:376184/149‑1 (MQ=255)
                                                                 gTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTAACTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGc                                                                             <  3:370279/149‑1 (MQ=255)
                                                                    gTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCt                                                                           <  7:250046/148‑1 (MQ=255)
                                                                      cGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCt                                                                        <  4:154035/149‑1 (MQ=255)
                                                                       gCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCt                                                                        <  6:213898/148‑1 (MQ=255)
                                                                        ccAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTCAATCTCTGGCGAGTTTTTCGCCTGCTTc                                                                      >  4:33218/1‑149 (MQ=255)
                                                                        ccAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCtt                                                                       >  8:303456/1‑148 (MQ=255)
                                                                        ccAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCtt                                                                       >  7:231628/1‑148 (MQ=255)
                                                                            tCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGGGTTTTTCGCCTGCTTCACg                                                                   >  1:295217/1‑148 (MQ=255)
                                                                              aGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAAt                                                                <  7:177871/149‑1 (MQ=255)
                                                                               gCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGaa                                                                 <  3:178680/147‑1 (MQ=255)
                                                                               gCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGaa                                                                 >  4:178680/1‑147 (MQ=255)
                                                                                cACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATgg                                                              >  5:922/1‑149 (MQ=255)
                                                                                 aCTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgc                                                             <  7:303456/149‑1 (MQ=255)
                                                                                   ttGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATctct                                                                                           <  5:20788/117‑1 (MQ=255)
                                                                                   ttGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATctct                                                                                           >  6:20788/1‑117 (MQ=255)
                                                                                   ttGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgcag                                                           >  8:136642/1‑149 (MQ=255)
                                                                                     gCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgcag                                                           <  7:136642/147‑1 (MQ=255)
                                                                                        gagagaGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATg                                                      >  2:285738/1‑149 (MQ=255)
                                                                                        gagagaGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATg                                                      >  8:202718/1‑149 (MQ=255)
                                                                                             agGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTaa                                                 <  8:371470/149‑1 (MQ=255)
                                                                                               gCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgcag                                                           >  4:322642/1‑137 (MQ=255)
                                                                                               gCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgcag                                                           <  3:322642/137‑1 (MQ=255)
                                                                                                cACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTg                                              >  6:98630/1‑149 (MQ=255)
                                                                                                 aCTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCAc                                                                                                                    <  4:118623/78‑1 (MQ=255)
                                                                                                 aCTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATgt                                                     <  5:290307/141‑2 (MQ=255)
                                                                                                 aCTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATgg                                                     >  6:290307/1‑141 (MQ=255)
                                                                                                 aCTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTgg                                             >  3:40664/1‑149 (MQ=255)
                                                                                                  cTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGgc                                            <  7:202718/149‑1 (MQ=255)
                                                                                                  cTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGgc                                            <  4:56925/149‑1 (MQ=255)
                                                                                                     aaCGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgc                                                             >  7:259304/1‑129 (MQ=255)
                                                                                                     aaCGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGgc                                                             <  8:259304/129‑1 (MQ=255)
                                                                                                     aaCGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCa                                         >  1:405925/1‑149 (MQ=255)
                                                                                                       cGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCAtt                                       <  4:40664/149‑1 (MQ=255)
                                                                                                          cTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGcc                                    <  8:231628/149‑1 (MQ=255)
                                                                                                             gtgtGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCa                                   <  1:285738/147‑1 (MQ=255)
                                                                                                                        gcCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTCACTGGCGCATTGCCACGCTTTTTAcccc                      >  2:123660/1‑149 (MQ=255)
                                                                                                                        gcCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAAccc                      <  6:922/149‑1 (MQ=255)
                                                                                                                        gcCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAAccc                      >  8:116144/1‑149 (MQ=255)
                                                                                                                          cTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCtt                    >  5:236848/1‑149 (MQ=255)
                                                                                                                           tGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTg                   >  7:112997/1‑149 (MQ=255)
                                                                                                                               cGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTgg               <  7:116144/149‑1 (MQ=255)
                                                                                                                                 aCGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGc             <  2:433521/149‑1 (MQ=255)
                                                                                                                                 aCGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACCAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGc             <  1:412162/149‑1 (MQ=255)
                                                                                                                                   gTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGa           >  8:438297/1‑149 (MQ=255)
                                                                                                                                     tttttGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACt         >  8:402607/1‑149 (MQ=255)
                                                                                                                                        ttGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACttt       <  8:112997/148‑1 (MQ=255)
                                                                                                                                        ttGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACttt       <  2:405925/148‑1 (MQ=255)
                                                                                                                                         tGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACTTTg      <  7:438297/148‑1 (MQ=255)
                                                                                                                                         tGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACTTTg      <  5:98630/148‑1 (MQ=255)
                                                                                                                                         tGCAAATTTATCGTCAACGCGCCCCATGCCGGGTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACTTTg      <  7:390426/148‑1 (MQ=255)
                                                                                                                                            aaaTTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACTTTGGCTg  >  6:222918/1‑149 (MQ=255)
                                                                                                                                              aTTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTg                                                                                          <  4:369272/59‑1 (MQ=255)
                                                                                                                                              aTTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTg                                                                                          >  3:369272/1‑59 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCGTTGTCGCCAGTCAGCACTTGCGGAGAGAGGCACTCAACGCCTGGTGTGCCGCGCGCCTGAGCGACGTTTTTTGCAAATTTATCTTCAACGCGCCCCATGCCGGTTTCACTGACGGCAAGTTCCGCTAAATCTCTGGCGTGTTTTTCGCCTGCTTCACGAATGGCAGCAATGGCTAACTGGCGCATTGCCACGCTTTTTAACCCTTGCTGGGCGACTTTGGCTG  >  NC_000913/2571339‑2571627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: