Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,322,076	A→C	77.6%	noncoding (83/87 nt)	leuU ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,322,076	0	A	C	77.6%	167.5 / 45.1	76	noncoding (83/87 nt)	leuU	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (6/11);  new base C (30/29);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

TAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCAGCTGGCTGA  >  NC_000913/3321934‑3322218                                                                                                                                               |                                                                                                                                               tAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAg                                                                                                                                           >  7:381438/1‑148 (MQ=255)     gCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACgg                                                                                                                                      <  2:311920/149‑1 (MQ=255)     gCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACgg                                                                                                                                      <  2:331252/149‑1 (MQ=255)       gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGa                                                                                                                                    <  3:96114/149‑1 (MQ=255)         aCCGGCACCACCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACt                                                                                                                                  >  7:97865/1‑149 (MQ=255)          ccGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACtt                                                                                                                                 <  8:76458/149‑1 (MQ=255)           cGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACtt                                                                                                                                 >  3:50579/1‑148 (MQ=255)             gCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGaa                                                                                                                              <  8:360897/149‑1 (MQ=255)                ccATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAAccc                                                                                                                           <  3:208439/149‑1 (MQ=255)                 cATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAAccc                                                                                                                           <  7:288284/148‑1 (MQ=255)                 cATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAAccc                                                                                                                           >  8:288284/1‑148 (MQ=255)                  aTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCg                                                                                                                          >  3:381733/1‑148 (MQ=255)                      tttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGc                                                                                                                     >  4:296484/1‑149 (MQ=255)                       ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGcc                                                                                                                    >  8:3062/1‑149 (MQ=255)                        tCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGcc                                                                                                                    <  4:50579/148‑1 (MQ=255)                        tCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGcc                                                                                                                    <  8:97865/148‑1 (MQ=255)                         cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGccc                                                                                                                   >  2:128274/1‑148 (MQ=255)                         cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACgg                                                                                                                                      >  7:74454/1‑129 (MQ=255)                         cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACgg                                                                                                                                      <  8:74454/129‑1 (MQ=255)                          aaGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                                                                                 >  4:75354/1‑149 (MQ=255)                            ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                                                                               >  1:30255/1‑149 (MQ=255)                            ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                                                                               <  8:381438/149‑1 (MQ=255)                             gTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                                              <  6:158074/149‑1 (MQ=255)                             gTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAACACGCTTTTAAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGTGACTTGAACCCGTAAGCCCTATTg                                                                                                              <  3:278258/149‑1 (MQ=255)                                ccAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTggg                                                                                                            <  7:3062/148‑1 (MQ=255)                                ccAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTggg                                                                                                            <  7:323754/148‑1 (MQ=255)                                       cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                                                                      <  4:157471/147‑1 (MQ=255)                                       cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                                                                      <  8:219930/147‑1 (MQ=255)                                       cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                                                                      <  3:306699/147‑1 (MQ=255)                                       cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTAGGCACTacc                                                                                                     <  8:277030/148‑1 (MQ=255)                                        gAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                                                   >  3:397873/1‑149 (MQ=255)                                           tAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCGCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTTGAATTTGGTCCCGACGACGCGACTTGAACCCGTAACCCCTATTGGGCACTCCAACCt                                                                                                 >  2:5716/1‑148 (MQ=255)                                              aGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                                                                                 >  7:178596/1‑129 (MQ=255)                                              aGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                                                                                 <  8:178596/129‑1 (MQ=255)                                                      gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                                                                      <  4:381733/148‑1 (MQ=255)                                                      gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                                                                      <  3:296484/148‑1 (MQ=255)                                                        cGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                                                                                   <  2:30255/149‑1 (MQ=255)                                                         gagaTACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCt                                                                                  <  3:75354/149‑1 (MQ=255)                                                           gatacagatacCTTACAACCATTCCTCAGCCCAGGACCAAAACGAAAAAAGAAGCTTTTCAGCGTCTGTTTTTTTGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                                                <  7:354794/149‑1 (MQ=255)                                                           gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                                                <  4:397873/149‑1 (MQ=255)                                                              acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCaa                                                                             >  3:217434/1‑149 (MQ=255)                                                               cagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                                                                            <  2:85302/149‑1 (MQ=255)                                                                  atacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                                                                         <  5:209542/149‑1 (MQ=255)                                                                   tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                                                                        <  7:275737/149‑1 (MQ=255)                                                                     ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                                                                       <  1:331475/148‑1 (MQ=255)                                                                              cATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCa                                                             >  3:334524/1‑149 (MQ=255)                                                                              cATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                                                                      >  4:92631/1‑124 (MQ=255)                                                                                   cTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGa                                                         >  7:122348/1‑148 (MQ=255)                                                                                      aGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAtac                                                      >  8:177919/1‑148 (MQ=255)                                                                                          cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtt                                                 >  6:194923/1‑149 (MQ=255)                                                                                            ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCCTGTATACCAATTCCACCACCTCGGCACGGAGTCTACtttt                                               >  6:128067/1‑149 (MQ=255)                                                                                            ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtttt                                               >  2:316480/1‑149 (MQ=255)                                                                                               ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtttt                                               >  4:102157/1‑146 (MQ=255)                                                                                               ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTa                                             <  3:102157/148‑1 (MQ=255)                                                                                                  aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTc                                         >  7:109147/1‑149 (MQ=255)                                                                                                    aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCgg                                       <  1:316480/149‑1 (MQ=255)                                                                                                      gAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTa                                             >  3:56893/1‑141 (MQ=255)                                                                                                      gAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTa                                             <  4:56893/141‑1 (MQ=255)                                                                                                       aaaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGa                                     >  5:331752/1‑148 (MQ=255)                                                                                                         aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCg                                        >  3:330951/1‑143 (MQ=255)                                                                                                         aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCg                                        <  4:330951/143‑1 (MQ=255)                                                                                                           aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCg                                >  4:114905/1‑149 (MQ=255)                                                                                                                  ttttCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCg                         >  2:256397/1‑149 (MQ=255)                                                                                                                  ttttCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTAC‑TTTTAGTTCGGGATATCGCTGGTCgg                        >  4:259715/1‑149 (MQ=255)                                                                                                                    ttCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCgg                        <  3:114905/148‑1 (MQ=255)                                                                                                                     tCAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGc                       <  1:128274/148‑1 (MQ=255)                                                                                                                      cAGCGTCTCTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCt                      <  6:331752/148‑1 (MQ=255)                                                                                                                       aGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCt                      >  6:391336/1‑147 (MQ=255)                                                                                                                           tctcTTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCg                >  7:220470/1‑149 (MQ=255)                                                                                                                            ctctTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCggg                                      <  7:71435/126‑1 (MQ=255)                                                                                                                            ctctTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCggg                                      <  7:64479/126‑1 (MQ=255)                                                                                                                            ctctTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCggg                                      >  8:64479/1‑126 (MQ=255)                                                                                                                            ctctTTTCTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCggg                                      >  8:71435/1‑126 (MQ=255)                                                                                                                                   cTGGAATTTGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCAgct        >  7:173455/1‑149 (MQ=255)                                                                                                                                    tGGAATTTGGTACCGAGGACGGGACTTGACCCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCAgctg       >  7:355957/1‑149 (MQ=255)                                                                                                                                         tttGGTCCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCAGCTGGCTGa  <  4:334524/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                               TAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGGAGCAGCTGGCTGA  >  NC_000913/3321934‑3322218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑