Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 462,737 | C→A | 5.4% | Q275K (CAG→AAG) | ppiD → | periplasmic folding chaperone, has an inactive PPIase domain |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 462,737 | 0 | C | A | 5.4% | 293.8 / 2.9 | 87 | Q275K (CAG→AAG) | ppiD | periplasmic folding chaperone, has an inactive PPIase domain |
Reads supporting (aligned to +/- strand): ref base C (35/47); new base A (2/3); total (37/50) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGACGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGATGCCA > NC_000913/462591‑462878 | tGACGCCGGAACAATTCCTCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAg < 2:56/149‑1 (MQ=255) aCGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGAc < 4:290562/149‑1 (MQ=255) aaCAATTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGAGGATATACAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACTAAAACt < 6:30305/149‑1 (MQ=255) cAATTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGa < 2:116208/149‑1 (MQ=255) aaTTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGaa < 7:37938/149‑1 (MQ=255) aaTTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGaa < 7:226490/149‑1 (MQ=255) aaTTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGaa < 6:371296/149‑1 (MQ=255) aaTTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGaa < 5:9769/149‑1 (MQ=255) ttCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTg > 7:260089/1‑145 (MQ=255) ttCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTg > 7:253664/1‑145 (MQ=255) gTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGc < 3:286174/149‑1 (MQ=255) tACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAg < 1:57830/148‑1 (MQ=255) aCATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCAGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCg > 4:361789/1‑149 (MQ=255) cATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCgg < 4:391981/149‑1 (MQ=255) cATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCg < 2:226684/148‑1 (MQ=255) aTCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCgg > 4:80260/1‑148 (MQ=255) aTCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCgg > 1:90604/1‑148 (MQ=255) aGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAGTTCACCCAGACGCAGCGTACCCGCTACAGCATCATCCATAACAAAACTGAAGATGAAGCGAAATCGGTACtt > 4:225670/1‑149 (MQ=255) cTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtga > 7:203263/1‑149 (MQ=255) tGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgat > 3:360248/1‑149 (MQ=255) tGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgat < 2:369086/149‑1 (MQ=255) gatatccGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtga < 1:153525/142‑1 (MQ=255) gATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgatga < 4:360248/149‑1 (MQ=255) gATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgatga < 1:101021/149‑1 (MQ=255) gATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgat < 6:329358/147‑1 (MQ=255) gATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgat < 6:217419/147‑1 (MQ=255) gATGCCGCAACGATGCAGCAACCGGTTAGCGATGCAGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTtgatga < 3:361789/149‑1 (MQ=255) ccGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCt < 5:1152/148‑1 (MQ=255) gCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGaa < 8:203263/149‑1 (MQ=255) gCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGaa > 7:79469/1‑149 (MQ=255) gCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGaa > 1:128561/1‑149 (MQ=255) gATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAg < 8:260089/149‑1 (MQ=255) gATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAg < 8:253664/149‑1 (MQ=255) gATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAg < 7:114376/149‑1 (MQ=255) agcaACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCAAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGt > 4:347780/1‑149 (MQ=255) agcaACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCAAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGt > 4:353535/1‑149 (MQ=255) aCCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGAtt > 3:75194/1‑149 (MQ=255) aCCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGAtt < 6:325746/149‑1 (MQ=255) aCCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGAtt > 8:43255/1‑149 (MQ=255) ttAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGACGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACGGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTtgct > 1:33256/1‑149 (MQ=255) gCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCa > 1:212529/1‑149 (MQ=255) cGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGc < 8:79469/148‑1 (MQ=255) ggATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTAATTGATGACCTGAATAAAGGCGGTGATTTTGATGCATTAgac > 8:110641/1‑146 (MQ=255) gATATCAAGAGCTACAACGACCAGCATCAGGATCAATTCACCCAGCCGCCGCGTACACGCTACAGCATCATCCAGCACAAAACTGAAGATGAAGCGAAAGCGGTACTTGAAGAGCTGAATAAAGGCTGTGATTTTGCTGCATTAGcaa > 5:255517/1‑146 (MQ=255) ccAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTtgc < 2:411296/132‑1 (MQ=255) ccAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTtgc > 1:411296/1‑132 (MQ=255) agagCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGGTGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGaaaaa > 3:384653/1‑149 (MQ=255) agagCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGaaaaa > 1:384820/1‑149 (MQ=255) agagCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGaaaaa > 6:201920/1‑149 (MQ=255) tactacGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGc > 3:219449/1‑149 (MQ=255) ctacGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGcc > 4:65043/1‑148 (MQ=255) ctacGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGc < 5:201920/147‑1 (MQ=255) cGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGat < 4:272519/148‑1 (MQ=255) cATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTAtctc < 4:368016/149‑1 (MQ=255) cATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTAtctc < 2:128561/149‑1 (MQ=255) aTCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATctct > 7:152076/1‑149 (MQ=255) tCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTg > 6:96605/1‑149 (MQ=255) gtaTCAATTCTCCCAGCCGCAGCGTAGCCGCTACAGCATCATGCAGACCAAAAGTGAAGATGAAGCGTAAGCGGTTCTTGATGAGCTGTATAAAGGCGGTGATTTTGCTGCATTAGCCAATGAAAAATCTGCCGATATTATCTCTGCTc < 2:77197/147‑1 (MQ=255) ggATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAg > 7:414040/1‑95 (MQ=255) ggATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAg < 8:414040/95‑1 (MQ=255) ggATCAAATCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTc < 6:114172/149‑1 (MQ=255) gATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCg > 1:192685/1‑149 (MQ=255) aTCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTc < 2:212529/147‑1 (MQ=255) aTCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCg > 7:144902/1‑148 (MQ=255) aaTTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAATATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAAc < 2:90604/149‑1 (MQ=255) aaTTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTaa < 2:384820/148‑1 (MQ=255) ttCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAAcgg > 8:5158/1‑149 (MQ=255) aCCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGAtattat > 2:166332/1‑131 (MQ=255) aCCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGAtattat < 1:166332/131‑1 (MQ=255) aCCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAAcggcgg > 4:153114/1‑149 (MQ=255) cccAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACggcggc > 7:147262/1‑149 (MQ=255) cccAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACggcggc < 8:152076/149‑1 (MQ=255) aGCCGCAGCGTACCCGCTACAGCATCATCAAGACCAAAACTGAAGATGAATCTAAATCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAATAAAAATCTGCCGATATTATCTCTGCTCGTAACTGCGGCGat < 6:255517/149‑1 (MQ=255) ccGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGatat < 3:65043/149‑1 (MQ=255) cGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATg > 8:263345/1‑149 (MQ=255) gCAGCGTACCCGCTACAGCATCCTCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATgg > 1:117570/1‑149 (MQ=255) cGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGggttg < 8:144902/149‑1 (MQ=255) cccGCTACAGCATCATCAAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTa < 3:353535/149‑1 (MQ=255) cccGCTACAGCATCATCAAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTa < 3:347780/149‑1 (MQ=255) ccGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTag < 7:110641/149‑1 (MQ=255) gCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTagaa < 7:219696/149‑1 (MQ=255) gCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTag > 1:380044/1‑147 (MQ=255) gCTACAGCAGCATCCAGACCAAAACTGAAGATTAAGCGAAAGCTGTACTTTATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTagaa < 3:225670/149‑1 (MQ=255) aCAGCATCATCCAGACCAAAACTGAAGCTGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCAGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGAt > 8:282365/1‑149 (MQ=255) cAGCATCATCCAGACCAAAACTGAAGGTGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGAt < 4:384653/148‑1 (MQ=255) aGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGATGc < 5:96605/149‑1 (MQ=255) catcatCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGATGCCa < 7:43255/149‑1 (MQ=255) | TGACGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGATGCCGCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAGGATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGATGAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAAGAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGATGCCA > NC_000913/462591‑462878 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |