Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)21 (0.600) 17/254 1.3 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 0 (0.000)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)36 (1.040) 32/252 0.2 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 0 (0.000)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

TTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAC  >  NC_000913/910940‑911234
                                                                                                                                                  |                                                                                                                                                    
ttAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGtt                                                                                                                                                     >  1:125776/1‑148 (MQ=255)
 tAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTa                                                                                                                                                   >  8:110257/1‑149 (MQ=255)
   aGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGt                                                                                                                                                 >  1:177338‑M2/1‑148 (MQ=255)
      cTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtgga                                                                                                                                              >  7:64206‑M2/1‑145 (MQ=255)
                   cGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatat                                                                                                                                 <  3:8696‑M2/149‑18 (MQ=255)
                       cAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttc                                                                                                                              >  7:110257‑M2/1‑128 (MQ=255)
                          agagTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTGTAGtggatttgcccctatatttccaga                                                                                                                          <  8:70934‑M2/149‑25 (MQ=255)
                              tCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGttgatttgcccctatatttc                                                                                                                              >  4:115671‑M2/1‑121 (MQ=255)
                              tCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttc                                                                                                                              <  3:115671‑M2/141‑21 (MQ=255)
                                     ccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAgg                                                                                                                                   <  3:151986‑M2/20‑1 (MQ=255)
                                     ccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAgg                                                                                                                                   >  4:151986‑M2/110‑129 (MQ=255)
                                        cTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGTCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcac                                                                                                             >  2:31821‑M2/1‑111 (MQ=255)
                                           cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgc                                                                                                                                         <  7:28672‑M2/117‑10 (MQ=255)
                                           cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgc                                                                                                                                         >  8:28672‑M2/1‑108 (MQ=255)
                                            ccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaac                                                                                                        >  4:8696‑M2/1‑107 (MQ=255)
                                            ccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaac                                                                                                        >  2:125776‑M2/1‑107 (MQ=255)
                                                    gTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattac                                                                                                 <  8:93051‑M2/148‑50 (MQ=255)
                                                     atctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAggg                                                                                                                                  <  3:128624‑M2/21‑1 (MQ=255)
                                                     atctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAggg                                                                                                                                  >  4:128624‑M2/94‑114 (MQ=255)
                                                        tgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTaaa                                                                                            >  1:185093‑M2/91‑149 (MQ=255)
                                                           cccTGCCGTTTCGAGTGGTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccct                                                                                                                                     >  8:115373‑M2/1‑92 (MQ=255)
                                                           cccTGCCGATTCGAGTGTTTTGGCGATATATGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccct                                                                                                                                     <  7:115373‑M2/105‑14 (MQ=255)
                                                             tccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGcc                                                                                       >  1:186861‑M2/86‑149 (MQ=255)
                                                              tGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatc                                                                                                               <  4:100805‑M2/124‑36 (MQ=255)
                                                              tGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatc                                                                                                               >  3:100805‑M2/1‑89 (MQ=255)
                                                               caatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGccc                                                                                      >  5:56818‑M2/84‑148 (MQ=255)
                                                                      tagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctat                                                                                                                                                      >  8:116694‑M2/77‑77 (MQ=255)
                                                                      tagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctat                                                                                                                                                      <  7:116694‑M2/1‑1 (MQ=255)
                                                                      tagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATggg                                                                                                                      <  6:158514‑M2/33‑1 (MQ=255)
                                                                      tagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATggg                                                                                                                      >  5:158514‑M2/77‑109 (MQ=255)
                                                                              ttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgt                                                                       >  6:123128‑M2/1‑73 (MQ=255)
                                                                                        aaGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgat                                                             <  3:89305‑M2/148‑86 (MQ=255)
                                                                                        aaGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaaccca                                                                                                     >  6:19635‑M2/1‑63 (MQ=255)
                                                                                        aaGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaaccca                                                                                                     <  5:19635‑M2/108‑46 (MQ=255)
                                                                                          gCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagccc                                                                                           >  1:133076‑M2/1‑61 (MQ=255)
                                                                                          gCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagccc                                                                                           <  2:133076‑M2/116‑56 (MQ=255)
                                                                                           caatatcacttaaataagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATCGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGAGGAAAGGAATATCATCGGGCATAAGGc                                                         >  4:84830‑M2/56‑149 (MQ=255)
                                                                                           cTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacc                                                         >  7:93051‑M2/1‑60 (MQ=255)
                                                                                                tcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCCTCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTa                                                    <  2:186861‑M2/99‑1 (MQ=255)
                                                                                                  acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATg                                                  >  3:104271‑M2/49‑149 (MQ=255)
                                                                                                   cttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCg                                                                   <  6:103485‑M2/84‑1 (MQ=255)
                                                                                                   cttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCg                                                                   >  5:103485‑M2/48‑131 (MQ=255)
                                                                                                               gTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggattcgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcga                                                              >  1:74647‑M2/1‑40 (MQ=255)
                                                                                                               gTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggattcgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcga                                                              <  2:74647‑M2/124‑85 (MQ=255)
                                                                                                                  ctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcg                                                    <  3:151693‑M2/131‑95 (MQ=255)
                                                                                                                  ctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcg                                                    >  4:151693‑M2/1‑37 (MQ=255)
                                                                                                                         tcCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccg                                                                        >  5:23032‑M2/1‑30 (MQ=255)
                                                                                                                         tcCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccg                                                                        <  6:23032‑M2/104‑75 (MQ=255)
                                                                                                                           cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggc                                                                    >  4:151073‑M2/1‑28 (MQ=255)
                                                                                                                           cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggc                                                                    <  3:151073‑M2/106‑79 (MQ=255)
                                                                                                                           cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccatta                                                                                                  <  6:156626‑M2/76‑49 (MQ=255)
                                                                                                                           cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccatta                                                                                                  >  5:156626‑M2/1‑28 (MQ=255)
                                                                                                                                         gccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAgg              >  6:150560‑M2/10‑146 (MQ=255)
                                                                                                                                         gccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAgg              <  5:95892‑M2/137‑1 (MQ=255)
                                                                                                                                         gccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAgg              <  5:150560‑M2/137‑1 (MQ=255)
                                                                                                                                         gccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATCCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAgg              >  6:95892‑M2/10‑146 (MQ=255)
                                                                                                                                                aGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaag      >  2:30240‑M2/1‑7 (MQ=255)
                                                                                                                                                aGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaag      <  1:30240‑M2/147‑141 (MQ=255)
                                                                                                                                                 aTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATCGCAGGAGCAGGCCAGTACGTaa   >  3:8484‑M2/2‑149 (MQ=255)
                                                                                                                                                  tttAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttc  <  5:110213‑M2/149‑145 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAC  >  NC_000913/910940‑911234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.