Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,159,199 G→T G444V (GGC→GTC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,1990GT98.0% 335.4 / NA 99G444V (GGC→GTC) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (50/47);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTT  >  NC_000913/1159055‑1159347
                                                                                                                                                |                                                                                                                                                    
cAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGtcg                                                                                                                                                    >  1:252737/1‑147 (MQ=255)
    tACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCgg                                                                                                                                              >  3:310912/1‑149 (MQ=255)
      ccAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGc                                                                                                                                            <  7:63172/149‑1 (MQ=255)
         gCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCg                                                                                                                                           >  5:50302/1‑147 (MQ=255)
          cGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAg                                                                                                                                        <  3:278142/149‑1 (MQ=255)
           gAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAACCTGGTCGCAGCGGGCGTAGt                                                                                                                                       >  1:263055/1‑149 (MQ=255)
              aTGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGt                                                                                                                                    <  4:5530/149‑1 (MQ=255)
               tGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGtt                                                                                                                                   <  2:252737/149‑1 (MQ=255)
                       gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGc                                                                                                                                 <  8:47522/143‑1 (MQ=255)
                       gCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGc                                                                                                                                 >  7:47522/1‑143 (MQ=255)
                         tctGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                                                                         >  3:130049/1‑149 (MQ=255)
                           tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTACGCTCGACGCAGGTATTACCCGGCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGAGCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                                                                         <  5:186735/147‑1 (MQ=255)
                           tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                                                                         <  4:449523/147‑1 (MQ=255)
                           tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                                                                         >  8:328510/1‑147 (MQ=255)
                           tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                                                                         <  4:310912/147‑1 (MQ=255)
                           tGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTg                                                                                                                         <  7:328510/147‑1 (MQ=255)
                              ttGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGtt                                                                                                                    <  3:91260/149‑1 (MQ=255)
                                gctgcATTAGGTGGTAAAGAAAACATTACTAACCTCGACGCATGAATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTAGCTGGTTCTGGTGtt                                                                                                                    >  8:117472/1‑147 (MQ=255)
                                 ctgcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCa                                                                                                                  <  2:242925/148‑1 (MQ=255)
                                   gcATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGc                                                                                                               >  6:411186/1‑149 (MQ=255)
                                          gtggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGAtttt                                                                                                         <  6:282812/148‑1 (MQ=255)
                                           tggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg                                                                                                       <  6:50302/149‑1 (MQ=255)
                                            ggtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg                                                                                                      >  4:81602/1‑149 (MQ=255)
                                             gtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg                                                                                                      >  2:273290/1‑148 (MQ=255)
                                             gtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg                                                                                                      >  2:438230/1‑148 (MQ=255)
                                             gtAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCgg                                                                                                      >  6:242442/1‑148 (MQ=255)
                                              tAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTa                                                                                                    <  4:130049/149‑1 (MQ=255)
                                                aaGAAAACATTAATAACATCGACGCATGTATTAACCGTCTGCGCGTCAGCGTTGCAGATTAGTCTAAATTGTATAAGTACGGCCTGAAGAAAATGTTCGCAGCTGTCTAAGTGGTTACTTGTTCTGGTCTTCAGTCGATTTTCGGTAc                                                                                                   >  5:291123/1‑148 (MQ=255)
                                                 aGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACt                                                                                                  >  3:179102/1‑148 (MQ=255)
                                                 aGAAAACATTACTAACCTCGACGCATGTATTACACGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACt                                                                                                  >  8:460369/1‑148 (MQ=255)
                                                   aaaaCATTTCTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGATGATGTGTCTAAAGGGGATCAGGCCGGGCTGAAGAAACTGGTCTCAGCGGGCGTAGTGGTTGCTGGTGCTGGTGTTGAGGCGATTTTCGGTACTaaa                                                                                               <  5:233410/149‑1 (MQ=255)
                                                     aaCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGATGATGTGTCTAAAGTGTATCAGGCCGGCCTGAAGAAACTGGTCTCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATc                                                                                             <  6:195003/149‑1 (MQ=255)
                                                            cTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg                                                                                                       >  8:243204/1‑132 (MQ=255)
                                                            cTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg                                                                                                       <  7:243204/132‑1 (MQ=255)
                                                            cTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAAc                                                                                      <  4:400220/149‑1 (MQ=255)
                                                               aCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCt                                                                                    >  2:487642/1‑148 (MQ=255)
                                                               aCCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCt                                                                                    <  5:242442/148‑1 (MQ=255)
                                                                ccTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCTGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTg                                                                                   >  8:208759/1‑148 (MQ=255)
                                                                 ctctACCCATGTAAAACCCGTCAGCGCGTCAGCTTAGGAGATGTTTCTAAAGTGGATCAGGCCGGCCTCAAGAAACTGCTCGCAGCGGGCGTAGAGGTTGCTGGTTCTCGTGAACAGGCGATATACGGTACAAAAACCTAAAAACTGa                                                                                  >  4:411903/1‑148 (MQ=255)
                                                                  tCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGt                                                                                                                                       <  3:194416/94‑1 (MQ=255)
                                                                  tCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGt                                                                                                                                       >  4:194416/1‑94 (MQ=255)
                                                                  tCGACCCATGTATTAACCGTCTGCGCGTCAGCGATGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGATGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATACGATAACCTGaa                                                                                 >  2:368671/1‑148 (MQ=255)
                                                                     aCGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGGTCTGGTGTTCAGGCGATTTTCGGTAATAAATCCGATAACCTGAAAAcc                                                                             <  2:470415/149‑1 (MQ=255)
                                                                      cGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCg                                                                            <  3:232228/149‑1 (MQ=255)
                                                                       gCATGTATTACCCGTCTGCGCGTCAGCGTTGCCGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCga                                                                           <  8:28576/149‑1 (MQ=255)
                                                                         aTGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAAcc                                                                             >  5:320856/1‑145 (MQ=255)
                                                                          tGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGa                                                                                          >  3:106491/1‑131 (MQ=255)
                                                                          tGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGa                                                                                          <  4:106491/131‑1 (MQ=255)
                                                                             aTTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCTCAGCGGGCGTAGTGGTTGCTGGTTATGGTGTTCAGGCGATTTTCGGTAGTAAATCCGATAACCTGAAAACCGAgatgga                                                                     >  5:76477/1‑149 (MQ=255)
                                                                             aTTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTATTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATACGAAAACCTGAAAACCGAgatgga                                                                     >  2:374472/1‑149 (MQ=255)
                                                                              ttACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                                                                    <  1:25286/149‑1 (MQ=255)
                                                                                 cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                                                                         >  4:80405/1‑141 (MQ=255)
                                                                                 cccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                                                                         <  3:80405/141‑1 (MQ=255)
                                                                                  ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAg                                                                 >  1:60221/1‑148 (MQ=255)
                                                                                  ccGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGt                                                                <  2:60221/149‑1 (MQ=255)
                                                                                   cGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGAt                                                                                         <  8:282995/123‑1 (MQ=255)
                                                                                   cGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGAt                                                                                         >  7:282995/1‑123 (MQ=255)
                                                                                    gTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAc                                                              <  7:460369/149‑1 (MQ=255)
                                                                                         cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACAt                                                            <  4:179102/146‑1 (MQ=255)
                                                                                            gTCAGCGTTGCTGAGGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCTGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTaa                                                      <  7:289530/149‑1 (MQ=255)
                                                                                             tCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAAc                                                     <  6:320856/149‑1 (MQ=255)
                                                                                               aGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCa                                                   <  5:411186/149‑1 (MQ=255)
                                                                                                         aTGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt                                          >  4:158234/1‑148 (MQ=255)
                                                                                                          tgtgTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa                                        <  1:438230/149‑1 (MQ=255)
                                                                                                            tgtCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGa                                      >  8:422603/1‑149 (MQ=255)
                                                                                                              tCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCCGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACg                                    >  6:463110/1‑149 (MQ=255)
                                                                                                              tCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCTTAAGACg                                    >  8:273067/1‑149 (MQ=255)
                                                                                                               cTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGt                                   >  8:440625/1‑149 (MQ=255)
                                                                                                                tAAAGTGGATCAGGCCTGCCTGAAGAAACTTGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                                                                    <  5:55572/115‑1 (MQ=255)
                                                                                                                tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATcc                                                                                            >  6:29435/1‑91 (MQ=255)
                                                                                                                tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATcc                                                                                            <  5:29435/91‑1 (MQ=255)
                                                                                                                tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgatggat                                                                    >  6:55572/1‑115 (MQ=255)
                                                                                                                tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGtt                                  >  1:49211/1‑149 (MQ=255)
                                                                                                                tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGtt                                  >  4:108317/1‑149 (MQ=255)
                                                                                                                  aaGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCTGTAACCACTAATCCGTAAGACGtt                                  <  7:117472/147‑1 (MQ=255)
                                                                                                                  aaGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt                                          <  2:261140/139‑1 (MQ=255)
                                                                                                                  aaGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGt                                          >  1:261140/1‑139 (MQ=255)
                                                                                                                   aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                               <  7:422603/149‑1 (MQ=255)
                                                                                                                   aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCCTAATCCg                                           <  8:370859/136‑1 (MQ=255)
                                                                                                                   aGTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCCTAATCCg                                           >  7:370859/1‑136 (MQ=255)
                                                                                                                    ggggATCAGGCCGGCCTGAAGAAACTGGTCCCAGCGGGCGTATGGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTgggg                              <  7:102905/147‑1 (MQ=255)
                                                                                                                    gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGTTGTTCAGGCGATTTTCGGTACTAAATCCTATAACGGGAACACCGAGATGGATGAATCCATCCGTAACCAAGAATCCGTAAGACgggggg                               >  8:353350/1‑143 (MQ=255)
                                                                                                                    gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa                                        >  2:508027/1‑139 (MQ=255)
                                                                                                                    gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTaa                                        <  1:508027/139‑1 (MQ=255)
                                                                                                                    gTGGATCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTggg                               >  3:456126/1‑148 (MQ=255)
                                                                                                                        aTCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc                          >  2:345965/1‑149 (MQ=255)
                                                                                                                         tCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc                          >  7:234822/1‑148 (MQ=255)
                                                                                                                         tCAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGAc                          >  2:402421/1‑148 (MQ=255)
                                                                                                                          cAGGCCGGCCTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt                         >  1:231498/1‑148 (MQ=255)
                                                                                                                            ggccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGgatcggaag                      >  7:33224/1‑142 (MQ=255)
                                                                                                                            agccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGGGTTCAGGCTATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTg                                 <  8:33224/137‑1 (MQ=255)
                                                                                                                              ccggccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGaaa                                                                                <  6:457325/89‑1 (MQ=255)
                                                                                                                                  tcTGATGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGGTCTGGTGTTCAGGCGATTTTCGGTACTAATTCCGATAACCTGAAATCCGAGATGGATGAGTACATCCGTAACCACTAATCGGTAAGACGTTGGGGAGACTAAGGCAGcc                <  3:411903/148‑1 (MQ=255)
                                                                                                                                  ccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                                                                         >  2:264857/1‑92 (MQ=255)
                                                                                                                                  ccTGAAGAAACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCgaga                                                                         <  1:264857/92‑1 (MQ=255)
                                                                                                                                        gaaACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatctgg          <  1:273290/149‑6 (MQ=255)
                                                                                                                                        gaaACTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATgg          <  3:158234/149‑1 (MQ=255)
                                                                                                                                            cTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgat                                                                        <  2:349342/83‑1 (MQ=255)
                                                                                                                                            cTGGTCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAgat                                                                        >  1:349342/1‑83 (MQ=255)
                                                                                                                                                gcgcAGCGGGCGTAGTGGTTGCGGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta  <  3:81602/149‑2 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTT  >  NC_000913/1159055‑1159347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: