Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)27 (0.940) 24/252 0.3 86.6% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 4 (0.140)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)22 (0.770) 20/250 0.6 84.1% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 4 (0.150)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

ATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACG  >  NC_000913/910939‑911230
                                                                                                                                                   |                                                                                                                                                
aTTAAGACTGTCGCTAAGACCGTTCAGAGAGTCGACTGTTAATACAAAGATGCGTTTCACACCTGCAGATTCGAGTGTTTTGGCGATATAAGCTTCAACCGTTTGTTTCATGGTTCTCCATATCCTGAATGTGATAACGGTATCAAGtt                                                                                                                                                 >  3:29012/1‑149 (MQ=255)
     gACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGGTTCACCCCTGCCGAGTCGCGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGTTTCTCCATCTCCTGAATGTGATCAAGGTAACAAGTTTAGtg                                                                                                                                            <  3:89091‑M2/149‑3 (MQ=255)
            aCTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgc                                                                                                                                     <  5:19771‑M2/149‑10 (MQ=255)
             cTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcc                                                                                                                                    <  7:110759‑M2/149‑11 (MQ=255)
                     cgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAgggg                                                                                                                             >  2:44878‑M2/127‑148 (MQ=255)
                                 tccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtaataagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGCGGGAAGGGATgg                                                                                                                   >  6:92984‑M2/115‑146 (MQ=255)
                                            cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcactta                                                                                                      >  8:110759‑M2/1‑108 (MQ=255)
                                            cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgccc                                                                                                                                   >  4:43708‑M2/1‑108 (MQ=255)
                                            cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgccc                                                                                                                                   <  3:43708‑M2/119‑12 (MQ=255)
                                              cAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatct                                                                                                                 >  8:908‑M2/1‑106 (MQ=255)
                                              cAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatct                                                                                                                 <  7:908‑M2/135‑30 (MQ=255)
                                                gATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGAt                                                                                                                     >  4:24897/1‑129 (MQ=255)
                                                gATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGAt                                                                                                                     <  3:24897/129‑1 (MQ=255)
                                                 aTGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccat                                                                                                >  6:19771‑M2/1‑103 (MQ=255)
                                                       tctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGtt                                                                                           >  1:10899‑M2/93‑148 (MQ=255)
                                                           ccccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctg                                                                                                                <  8:61638‑M2/123‑31 (MQ=255)
                                                           ccccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctg                                                                                                                >  7:61638‑M2/1‑93 (MQ=255)
                                                             ctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGc                                                                                    >  2:24703‑M2/87‑149 (MQ=255)
                                                             ccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCAGGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGgggatttgcccctatatttccagccatctgttagcactgaacccattacaagcccgct                                                                                    <  8:85280‑M2/149‑59 (MQ=255)
                                                              cTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctg                                                                                   <  6:18487‑M2/149‑60 (MQ=255)
                                                                      ctagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggcgccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGaaa                                                                           <  2:10899‑M2/72‑1 (MQ=255)
                                                                        gAGTGGTTTGGCGATATAAGATGCAACCGTTTCTTGCATGGCTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttctcacttaacccattacaagcccgctgccgcagata                                                                          >  4:89091‑M2/1‑80 (MQ=255)
                                                                             aaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAggg                                                                                                                       <  2:157032‑M2/28‑1 (MQ=255)
                                                                             aaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAggg                                                                                                                       >  1:157032‑M2/71‑98 (MQ=255)
                                                                              aaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGCCGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATatc                                                                   <  2:91442‑M2/80‑1 (MQ=255)
                                                                                tGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtg                                                                  >  5:18487‑M2/1‑72 (MQ=255)
                                                                                   tagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAggg                                                                                                                              <  3:75763‑M2/21‑1 (MQ=255)
                                                                                    gATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccatta                                                                                              >  6:122919‑M2/1‑68 (MQ=255)
                                                                                        ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCAt                                                          >  1:54237‑M2/60‑148 (MQ=255)
                                                                                                aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTaaa                                                                                        <  2:70619/110‑1 (MQ=255)
                                                                                                aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTaaa                                                                                        >  1:70619/1‑110 (MQ=255)
                                                                                                   acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGgaga                                                                                                               <  5:111082‑M2/36‑1 (MQ=255)
                                                                                                   acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGgaga                                                                                                               >  6:111082‑M2/49‑84 (MQ=255)
                                                                                                   acttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATg                                              >  1:42636‑M2/49‑149 (MQ=255)
                                                                                                       atttaagtgatattggttgtctggagactcaggggcccagtctaTTTAGATCATCTGACGGAGGCGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCga                                           <  1:44878‑M2/104‑1 (MQ=255)
                                                                                                             gtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGatgatat                                                                          <  4:45937‑M2/73‑4 (MQ=255)
                                                                                                             gtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGatgatat                                                                          >  3:45937‑M2/39‑108 (MQ=255)
                                                                                                                 gtctccATCTCCTGAATGTTATCACGGTAACAAGTTTAGtggatgtgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgacgcagatattcacgtggcgagcgataacccagcgcactatgcggatgcca                                >  4:29012‑M2/2‑39 (MQ=255)
                                                                                                                       ttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGc                          >  3:68975‑M2/29‑149 (MQ=255)
                                                                                                                          tctggcgattcagggggccagtctaTTTACCTCAGCTGACGGAGCGCGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa                       <  4:68975‑M2/124‑1 (MQ=255)
                                                                                                                            tggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATg                     >  7:115374‑M2/24‑149 (MQ=255)
                                                                                                                             ggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg                    >  1:39084‑M2/23‑149 (MQ=255)
                                                                                                                              gagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATGGCCCGATGAAAg                                                                          >  1:15647‑M2/22‑94 (MQ=255)
                                                                                                                              gagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATg                     <  2:42636‑M2/126‑1 (MQ=255)
                                                                                                                              gAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgc                   <  5:79573‑M2/149‑124 (MQ=255)
                                                                                                                                        gggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc         <  8:115374‑M2/138‑1 (MQ=255)
                                                                                                                                        gggccacgctaTTTACTTCATCTGCCCGAGGGGGACGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc         <  2:39084‑M2/138‑1 (MQ=255)
                                                                                                                                          ggAAACAAGTTTAGtggatgtgcccctatatttccagacatctgttctcacttaacccattccaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctct       >  7:85280‑M2/1‑14 (MQ=255)
                                                                                                                                            cagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAg           <  3:71561‑M2/136‑1 (MQ=255)
                                                                                                                                            cagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAg           >  4:71561‑M2/8‑143 (MQ=255)
                                                                                                                                              gtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTAc   >  3:17762‑M2/6‑149 (MQ=255)
                                                                                                                                               cAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaag  <  4:125261‑M2/149‑141 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ATTAAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACG  >  NC_000913/910939‑911230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.