New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 394843426 (1.030)4 (0.170) 4/238 2.4 14.0% intergenic (+10/+15) yifE/yifB UPF0438 family protein/magnesium chelatase family protein and putative transcriptional regulator
?NC_000913 = 3948439 25 (1.060)intergenic (+15/+10) yifE/yifB UPF0438 family protein/magnesium chelatase family protein and putative transcriptional regulator
Rejected: Frequency below/above cutoff threshold.

AGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTC  >  NC_000913/3948291‑3948575
                                                                                                                                                    |                                                                                                                                        
aGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGATGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAAt                                                                                                                                          >  2:19604/1‑149 (MQ=255)
            aaGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTa                                                                                                                              <  5:2070/149‑1 (MQ=255)
                       gtgtGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGtt                                                                                                                   >  6:89814/1‑149 (MQ=255)
                        tgtgGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGttt                                                                                                                  <  5:89814/149‑1 (MQ=255)
                                       tGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGa                                                                                                    <  6:80074/148‑1 (MQ=255)
                                         aCGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGATGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGc                                                                                                  <  1:19604/148‑1 (MQ=255)
                                                ttAAGCGTCCAAAACGTTTTCATACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTAt                                                                                                                             <  1:32161/114‑1 (MQ=255)
                                                ttAAGCGTCCAAAACGTTTTCATACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTAt                                                                                                                             >  2:32161/1‑114 (MQ=255)
                                                    gCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTa                                                                                                                              >  4:81683/1‑109 (MQ=255)
                                                    gCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTa                                                                                                                              <  3:81683/109‑1 (MQ=255)
                                                           aaaCGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGa                                                                                >  1:102318/1‑148 (MQ=255)
                                                                       acCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGcct                                                                   >  8:43703/1‑149 (MQ=255)
                                                                       acCCATTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACCCCCATTCTGACCATAAAAAAAAAGGGCGAAATGCCCTTTTTTTAAGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCCATAGCTAACTGgct                                                                   >  6:35859/1‑149 (MQ=255)
                                                                         ccTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCATTTCgccctttttattaatcgtca                                                                                                                      <  6:30694‑M2/96‑21 (MQ=255)
                                                                         ccTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCATTTCgccctttttattaatcgtca                                                                                                                      >  5:30694‑M2/1‑76 (MQ=255)
                                                                                   ggTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCt                                                       <  7:43703/149‑1 (MQ=255)
                                                                                    gTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCTCTCGATAGCTAACTGCCTCCTGCAAATGCt                                                       >  3:78016/1‑148 (MQ=255)
                                                                                          cGCAGGTTGAAGGTGCTGAAGACTACTCCGATTCTGACGATAAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGTTGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACgtgt                                                <  5:35859/149‑1 (MQ=255)
                                                                                                            aaGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGa                                <  2:102318/147‑1 (MQ=255)
                                                                                                            aaGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGAt                               <  1:134627/148‑1 (MQ=255)
                                                                                                                    acCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAc                                                    >  4:104050/1‑119 (MQ=255)
                                                                                                                    acCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAc                                                    <  3:104050/119‑1 (MQ=255)
                                                                                                                              aCGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCaaa                                                           >  4:62954/1‑102 (MQ=255)
                                                                                                                              aCGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCaaa                                                           <  3:62954/102‑1 (MQ=255)
                                                                                                                                     ataaAAAGGGCGAAATgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgtttt                                                    >  4:60841‑M2/1‑16 (MQ=255)
                                                                                                                                     ataaAAAGGGCGAAATgccctttttattaatcgtcagaatcggtgtagtcttcagcaccttcaacctgcggtttaccgccggaaagggtgtgaaaacgtttt                                                    <  3:60841‑M2/102‑87 (MQ=255)
                                                                                                                                      taaAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGcctc                                                                  >  7:70608/1‑87 (MQ=255)
                                                                                                                                      taaAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGcctc                                                                  <  8:70608/87‑1 (MQ=255)
                                                                                                                                        aaaaGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACttt   >  7:71973/1‑148 (MQ=255)
                                                                                                                                        aaaaGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTc  <  8:34303/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
AGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTC  >  NC_000913/3948291‑3948575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.