Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,2560CT20.0% 58.9 / 10.3 30A102A (GCC→GCT) csgBcurlin nucleator protein, minor subunit in curli complex
Reads supporting (aligned to +/- strand):  ref base C (12/12);  new base T (3/3);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GACTAATAATAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAGTCGCAAATGGCTATTCGCGTGAC  >  NC_000913/1104109‑1104396
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gACTAATAATAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCa                                                                                                                                             <  7:56378/149‑1 (MQ=255)
 aCTAATAATAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAg                                                                                                                                            >  2:86886/1‑149 (MQ=255)
    aataatAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTATGGCGGTTGTTGCGCAAGAAGGTATTAGAAACCGGGCAAAGATTGACCAGACAGGAGCTTATAACCTTGCATATATTCATCAGGCGGGCAGTGCCAACGATGCTAGAAt                                                                                                                                         >  7:30827/1‑149 (MQ=255)
               tCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTg                                                                                                                              >  6:58334/1‑149 (MQ=255)
                               ggCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGc                                                                                                              <  6:5765/149‑1 (MQ=255)
                                gCTCTAAACTTTTGGCGGTTGTTGCGCAAGACGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCTAGTATTTCGCAAGGTGCTTATGGTAATACTGc                                                                                                              <  8:30827/148‑1 (MQ=255)
                                    aaaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCATCGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCgatga                                                                                                         >  2:118804/1‑149 (MQ=255)
                                     aaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCgatgat                                                                                                        >  8:8571/1‑149 (MQ=255)
                                       aCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTa                                                                                                      <  5:58334/149‑1 (MQ=255)
                                                ggTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAg                                                                                             >  3:100901/1‑149 (MQ=255)
                                                                      agCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAatat                                                                       >  3:29253/1‑149 (MQ=255)
                                                                        cAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTa                                                                     <  5:28985/149‑1 (MQ=255)
                                                                            cGGGCAAAGACTGACCAGACACGAGACTATAACCTTGCATATATTGATCCGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGACAGGTTCTGGTAATAAAGCAAATATTAcaca                                                                 <  4:29253/149‑1 (MQ=255)
                                                                                       tGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCgatga                                                                                                         >  6:69992/1‑98 (MQ=255)
                                                                                       tGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCgatga                                                                                                         <  5:69992/98‑1 (MQ=255)
                                                                                         aCCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCa                                                     <  1:86886/148‑1 (MQ=255)
                                                                                             gACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCgat                                                                                                           <  5:32165/90‑1 (MQ=255)
                                                                                             gACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCgat                                                                                                           >  6:32165/1‑90 (MQ=255)
                                                                                             gACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCaaaaaa                                                <  7:8571/149‑1 (MQ=255)
                                                                                                      ttATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCga                                                                                                            >  1:47675/1‑80 (MQ=255)
                                                                                                      ttATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCga                                                                                                            <  2:47675/80‑1 (MQ=255)
                                                                                                                       ttGATCAGGCGGGCAGTGCCAACGATGCTAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAGTCg                      >  2:96915/1‑149 (MQ=255)
                                                                                                                       ttGATCAGGCGGGCAGTGCCAACGATGCTAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAGTCg                      >  2:95012/1‑149 (MQ=255)
                                                                                                                           tCAGGCGGGCAGTGACAACGATGCCAGTATTTCGCAAGGTGCTTAAGGTAATACTGAGATGATTATCCAGAAAGGTGCTGGTAATAAAGCAAGAATTACACAGTATGGTCCTCAAAAAACGGCAATTGTAGTGCAGAGACCGTCGCgaa                  >  8:94280/1‑149 (MQ=255)
                                                                                                                               gCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGtaat                                                                                  <  2:121885/81‑1 (MQ=255)
                                                                                                                               gCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGtaat                                                                                  >  1:121885/1‑81 (MQ=255)
                                                                                                                                       tGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGc                                 <  7:60111/122‑1 (MQ=255)
                                                                                                                                       tGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGc                                 >  8:60111/1‑122 (MQ=255)
                                                                                                                                           aaCGATGCTAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAGTCGCAAATGGCTATTCGCGTGac  <  1:96915/149‑1 (MQ=255)
                                                                                                                                           aaCGATGCTAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAGTCGCAAATGGCTATTCGCGTGac  <  1:95012/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GACTAATAATAGTGCTCAGTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCTTATGGTAATACTGCGATGATTATCCAGAAAGGTTCTGGTAATAAAGCAAATATTACACAGTATGGTACTCAAAAAACGGCAATTGTAGTGCAGAGACAGTCGCAAATGGCTATTCGCGTGAC  >  NC_000913/1104109‑1104396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑