New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1757141 =	123 (1.160)	6 (0.060)	5/270	12.6	5.0%	intergenic (+31/‑280)	pykF/lpp	pyruvate kinase I/murein lipoprotein
	?	NC_000913	1757161 =	110 (1.090)					intergenic (+51/‑260)	pykF/lpp	pyruvate kinase I/murein lipoprotein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATCGAACGCTGGGTTTTAGATAAAAAAATTGAGAGAAAACAGGATGCTTCCATCGGATTCATCTTAGATAAAGTGAGATTATATAGTGAAATCCGATTTGATTTTTTATTGCTTCTGGTTATCGATTAAATAAAAAAAGCGCCCATCAGGGCGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1757296‑1757141 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcttTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAG  >  NC_000913/1757161‑1757307                                                                                                                                                                                                                                                                                                                 ATCGAACGCTGGGTTTTAGATAAAAAAATTGAGAGAAAACAGGATGCTTCCATCGGATTCATCTTAGATAAAGTGAGATTATATAGTGAAATCCGATTTGATTTTTTATTGCTTCTGGTTATCGATTAAATAAAAAAAGCGCCCATCAG                                                                                                                                                            >  7:95023/1‑149  TCGAACGCTGGGTTTTAGATAAAAAAATTGAGAGAAAACAGGATGCTTCCATCGGATTCATCTTAGATAAAGTGAGATTATATAGTGAAATCCGATTTGATTTTTTATTGCTTCTGGTTATCGATTAAATAAAAAAAGCGCCCATCAGG                                                                                                                                                           >  3:312756/1‑149  TCGAACGCTGGGTTTTAGATAAAAAAATTGAGAGAAAACAGGATGCTTCCATCGGATTCATCTTAGATAAAGTGAGATTATATAGTGAAATCCGATTTGATTTTTTATTGATTCTGGTTATCGATTAAATAAAAAAAGCGCCCATCAGG                                                                                                                                                           >  1:105081/1‑149    GAACGCTGGGTTTTAGATAAAAAAATTGAGAGAAAACAGGATGCTTCCATCGGATTCATCTTAGATAAAGTGAGATTATATAGTGAAATCCGATTTGATTTTTTATTGCTTCTGGTTATCGATTAAATAAAAAAAGCGCCCATCAGGG                                                                                                                                                          <  5:311830/148‑1                                                                                                                              TTAAATAAAAAAAGCGCCCATCAGGGGGCTTTTTTTATTTAATCGACAACAAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACGTTATCTAAGATGAAGACGATGGATGCATCCTGTTTTATCTCAATTTTTTTATC                               >  3:311934/1‑149                                                                                                                              TTAAATAAAAAAAGCGCCCATCAGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATC                                                                                         <  5:216399/91‑1                                                                                                                              TTAAATAAAAAAAGCGCCCATCAGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATC                                                                                         >  6:216399/1‑91                                                                                                                                    AAAAAAAGCGCCCATCAGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAAC                         >  4:23613/1‑149                                                                                                                                     AAAAAAGCGCCCCTCAGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTACACTATATAATCTCAATTTATCTAATATGAATCCGATGGAAGCATACTGTTTTCTCTAAATTTTTTTATCTAACACC                        >  3:256771/1‑149                                                                                                                                                cggaAGGGCGCTTTTTTTATTTATTCGATAACCAGAAGCAATAAAAAATAATATCGGATTTCACTATATTATCTCACTTTATGTTAGATGAATCCGATGGAAGCTTCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGAT             <  4:228548/145‑1                                                                                                                                                     GGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTC        >  1:379891/1‑149                                                                                                                                                      GGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTC        <  7:387708/148‑1                                                                                                                                                       GCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTT      >  1:250702/1‑149                                                                                                                                                       GCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTT      >  2:227166/1‑149                                                                                                                                                        CGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTT     <  8:225914/149‑1                                                                                                                                                         GCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTT     >  6:168907/1‑148                                                                                                                                                          CTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAAACCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTT     >  2:36676/1‑147                                                                                                                                                           TTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAG  >  7:378059/1‑149                                                                                                                                                                                                                                                                                                                 ATCGAACGCTGGGTTTTAGATAAAAAAATTGAGAGAAAACAGGATGCTTCCATCGGATTCATCTTAGATAAAGTGAGATTATATAGTGAAATCCGATTTGATTTTTTATTGCTTCTGGTTATCGATTAAATAAAAAAAGCGCCCATCAGGGCGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1757296‑1757141 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcttTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAG  >  NC_000913/1757161‑1757307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.