BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469,009	G→T	V266F (GTT→TTT)	gtrB →	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469,009	0	G	T	100.0%	400.8 / NA	110	V266F (GTT→TTT)	gtrB	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (55/55); total (55/55)

Only 100 of 113 total aligned reads displayed.

TATTACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCA  >  NC_000913/2468864‑2469154

tATTACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACtttttt                                                                                                                                                <  2:284540/149‑1 (MQ=255)

tATTACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACtttttt                                                                                                                                                <  7:328222/149‑1 (MQ=255)

 aTTACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACtttttt                                                                                                                                                >  2:394885/1‑148 (MQ=255)

  ttACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGTGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTATTGGATATACTTCACTAATTTTTTc                                                                                                                                               >  1:14441/1‑148 (MQ=255)

    aCAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCaa                                                                                                                                             <  2:220986/148‑1 (MQ=255)

    aCAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCaa                                                                                                                                             <  7:50725/148‑1 (MQ=255)

     cAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCaa                                                                                                                                             >  4:47226/1‑147 (MQ=255)

     cAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCaa                                                                                                                                             <  3:47226/147‑1 (MQ=255)

     cAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATa                                                                                                                                           >  7:219768/1‑149 (MQ=255)

            ttCCACATTCCCTCTTCTCATCTGTACCTACATAGGGTAAGTCGTAGCCAGTTTAGCATATATTTATGTGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGtt                                                                                                                                      <  6:18402/147‑1 (MQ=255)

               cacaTTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAg                                                                                                                                 >  5:73700/1‑149 (MQ=255)

                acaTTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAgg                                                                                                                                >  8:346230/1‑149 (MQ=255)

                 caTTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGt                                                                                                                               >  1:183662/1‑149 (MQ=255)

                 aaTTGCCTCTTGGGATTTTGACATACATTGGGTTTGTGGGATCCATTTGATCATTTATTTATGGGGAGTTTATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGt                                                                                                                               <  8:196758/148‑1 (MQ=255)

                  aTTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTg                                                                                                                              <  2:247904/149‑1 (MQ=255)

                   ttCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTg                                                                                                                              >  2:354229/1‑148 (MQ=255)

                      ccTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGaa                                                                                                                           <  6:234436/148‑1 (MQ=255)

                      ccTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAt                                                                                                                          >  2:326158/1‑149 (MQ=255)

                       ctctTCGCATCTGGAGATAGTTAGGTTTAGTGGTAGCCAGTGTTGCATTTTTTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATGCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAtt                                                                                                                         <  2:183662/149‑1 (MQ=255)

                       ctctTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAt                                                                                                                          >  2:171663/1‑148 (MQ=255)

                            cGCATCTGGACATACATAGGGTTAGTTGTAGACAGTGTAGCATTTATTTATGGGGCGTTGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCa                                                                                                                       <  6:120671/146‑1 (MQ=255)

                             gCATATGGACATACATAGGGTTAGTGGTCGCCAGTGTAGCTTTTATTTATCGGGCGTGGATGATTTTAGATACTCTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAgat                                                                                                                    <  4:101548/148‑1 (MQ=255)

                               aTCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAgatgat                                                                                                                 <  8:402022/149‑1 (MQ=255)

                               aTCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGCGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCGTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAgatgat                                                                                                                 <  1:378163/149‑1 (MQ=255)

                               aTCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTAATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGt                                                                                                                               >  7:346306/1‑135 (MQ=255)

                                   ggACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTgg                                                                                                              >  1:203613/1‑148 (MQ=255)

                                    gACATACATAGGGTTAGTGGTTGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTgg                                                                                                              <  5:194932/147‑1 (MQ=255)

                                    gACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGTGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGAtt                                                                                                                <  5:317325/145‑1 (MQ=255)

                                    gACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGAtt                                                                                                                <  7:20605/145‑1 (MQ=255)

                                    gACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGaa                                                                                                            <  6:47850/149‑1 (MQ=255)

                                      catacataGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATa                                                                                                          <  2:203613/149‑1 (MQ=255)

                                       atacataGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAg                                                                                                         <  1:378876/149‑1 (MQ=255)

                                        tacataGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAgg                                                                                                        <  5:300923/149‑1 (MQ=255)

                                          cataGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGa                                                                                                       <  4:47822/148‑1 (MQ=255)

                                             aGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAt                                                                                                   >  4:402007/1‑149 (MQ=255)

                                              gggTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTAtt                                                                                                  <  6:428934/149‑1 (MQ=255)

                                                 ttAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAgg                                                                                               <  2:10452/149‑1 (MQ=255)

                                                 ttAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAgg                                                                                               >  7:310813/1‑149 (MQ=255)

                                                     tGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGaa                                                                                           >  6:127375/1‑149 (MQ=255)

                                                     tGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGaa                                                                                           <  2:390816/149‑1 (MQ=255)

                                                       gTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTg                                                                                                                              >  7:12481/1‑112 (MQ=255)

                                                       gTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTg                                                                                                                              <  8:12481/112‑1 (MQ=255)

                                                       gTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGGTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAata                                                                                         >  2:427955/1‑149 (MQ=255)

                                                        tAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAatat                                                                                        <  6:161000/149‑1 (MQ=255)

                                                            cAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATAtt                                                                                     <  1:394885/148‑1 (MQ=255)

                                                             aGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTg                                                                                    >  8:234844/1‑148 (MQ=255)

                                                              gtgtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGTGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGa                                                                                  <  1:51233/149‑1 (MQ=255)

                                                               tgtAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGAc                                                                                 >  5:384924/1‑149 (MQ=255)

                                                                   gCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAgg                                                                                                                                <  7:186815/98‑1 (MQ=255)

                                                                   gCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAgg                                                                                                                                >  8:186815/1‑98 (MQ=255)

                                                                   gCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGca                                                                              >  7:183294/1‑148 (MQ=255)

                                                                      tttatttaTGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTTGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                          >  6:92901/1‑149 (MQ=255)

                                                                      tttatttaTGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacat                                                                           >  8:327472/1‑148 (MQ=255)

                                                                       ttatttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacata                                                                          >  8:261164/1‑148 (MQ=255)

                                                                         atttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCacatacat                                                                       >  1:314521/1‑149 (MQ=255)

                                                                          tttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAat                                                                                          >  1:267844/1‑129 (MQ=255)

                                                                          tttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAat                                                                                          <  2:267844/129‑1 (MQ=255)

                                                                          tttatGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACAtt                                                                      >  6:244619/1‑149 (MQ=255)

                                                                              tGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaaa                                                                  <  1:326158/149‑1 (MQ=255)

                                                                              tGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaa                                                                   <  2:228131/148‑1 (MQ=255)

                                                                              tGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGaa                                                                   <  3:321170/148‑1 (MQ=255)

                                                                                       gatgatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCaaaaaa                                                          <  3:198593/148‑1 (MQ=255)

                                                                                          gatTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGcc                                                      >  5:152938/1‑149 (MQ=255)

                                                                                                aGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGaaa                                                 <  2:102441/148‑1 (MQ=255)

                                                                                                aGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGaaa                                                 <  7:412121/148‑1 (MQ=255)

                                                                                                 gATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAAt                                                <  3:402007/148‑1 (MQ=255)

                                                                                                      tATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTg                                                                                                               >  7:446205/1‑80 (MQ=255)

                                                                                                      tATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatca                                          <  1:152701/149‑1 (MQ=255)

                                                                                                      tATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatca                                          >  3:168454/1‑149 (MQ=255)

                                                                                                      tATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatca                                          >  4:253493/1‑149 (MQ=255)

                                                                                                      tATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatca                                          >  4:279892/1‑149 (MQ=255)

                                                                                                       aTCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatcat                                         >  4:205619/1‑149 (MQ=255)

                                                                                                        tCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatcat                                         >  2:366381/1‑148 (MQ=255)

                                                                                                        tCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATAcatcat                                         >  7:379839/1‑148 (MQ=255)

                                                                                                        tCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACatcatc                                        <  3:205619/149‑1 (MQ=255)

                                                                                                           tatTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACatcatc                                        >  4:370078/1‑146 (MQ=255)

                                                                                                             tttGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAaga                                    <  5:244619/148‑1 (MQ=255)

                                                                                                             tttGGAAATGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAaga                                    <  8:186642/148‑1 (MQ=255)

                                                                                                                     tGCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAAATCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAACCGCCCGAAATACATCATCAAGAGAGTCaaa                            >  4:115254/1‑148 (MQ=255)

                                                                                                                      gCTGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCaaaa                           >  4:148343/1‑148 (MQ=255)

                                                                                                                       cTGTTATGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCaaaaaa                         >  3:95663/1‑149 (MQ=255)

                                                                                                                        tGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAAt                        >  4:237833/1‑149 (MQ=255)

                                                                                                                        tGTTAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAAt                        >  4:375354/1‑149 (MQ=255)

                                                                                                                          ttAGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAAt                        <  3:370078/147‑1 (MQ=255)

                                                                                                                            aGGGGATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGaa                     <  3:375354/148‑1 (MQ=255)

                                                                                                                             ggggATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAAt                    <  7:234844/148‑1 (MQ=255)

                                                                                                                             ggggATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACAGACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAAt                    >  6:154325/1‑148 (MQ=255)

                                                                                                                              gggATATCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATa                   >  2:17986/1‑148 (MQ=255)

                                                                                                                                    tCCTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCaa             >  7:7904/1‑148 (MQ=255)

                                                                                                                                     ccTTCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTTGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCACGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAAt            >  4:333977/1‑148 (MQ=255)

                                                                                                                                       ttCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaa          >  5:119053/1‑148 (MQ=255)

                                                                                                                                       ttCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaa          >  5:368959/1‑148 (MQ=255)

                                                                                                                                       ttCACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGAAATaa          >  1:96475/1‑148 (MQ=255)

                                                                                                                                         cACTACTTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaaaa        <  8:219768/148‑1 (MQ=255)

                                                                                                                                            tactTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTa     >  1:186360/1‑148 (MQ=255)

                                                                                                                                            tactTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGACAAAAAATGAATAAAGCAATAAAAGTa     >  7:358526/1‑148 (MQ=255)

                                                                                                                                             actTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATATGAGTATTAGGTGAATATATTGGACGCACATACTTTGAAACCAAAAATAGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATc   <  2:14441/149‑1 (MQ=255)

                                                                                                                                              ctTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTAt    <  8:379839/147‑1 (MQ=255)

                                                                                                                                              ctTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCa  <  7:261164/149‑1 (MQ=255)

                                                                                                                                              ctTTTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCa  <  6:384924/149‑1 (MQ=255)

TATTACAAGCTTTTCCACATTCCCTCTTCGCATCTGGACATACATAGGGTTAGTGGTAGCCAGTGTAGCATTTATTTATGGGGCGTGGATGATTTTAGATACTATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCA  >  NC_000913/2468864‑2469154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`