Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,668	Δ1 bp	coding (716/2661 nt)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,666	0	C	.	100.0%	76.4 / NA	18	coding (714/2661 nt)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/9);  total (9/9)

ATGAATTGCTTGACTATCTGGCACGCGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAGCCCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCC  >  NC_000913/2720518‑2720790                                                                                                                                                     |                                                                                                                             atgaATTGCTTGACTATCTGGCACGCGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAGc                                                                                                                              >  3:13273/1‑149 (MQ=255)          ttGACTATCTGGCACGCGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGca                                                                                                                     >  2:157895/1‑148 (MQ=255)                         gcgACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAaca                                                                                                     <  6:61718/149‑1 (MQ=255)                               gTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACgg                                                                                               >  7:48250/1‑149 (MQ=255)                                  aaaCCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACgg                                                                                               >  3:118335/1‑146 (MQ=255)                                  aaaCCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCa                                                                                             <  4:118335/148‑1 (MQ=255)                                   aaCCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACACTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCTATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAgg                                                                                           <  7:104140/149‑1 (MQ=255)                                     ccAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGaa                                                                                         <  1:157895/149‑1 (MQ=255)                                                      tATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATgg                                                                           >  2:148050/1‑146 (MQ=255)                                                      tATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATgg                                                                           <  1:148050/146‑1 (MQ=255)                                                                  ttAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAg                                                            >  1:80263/1‑149 (MQ=255)                                                                                      tGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCgg                                          <  4:13273/147‑1 (MQ=255)                                                                                        tttCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCgg                                          >  5:101146/1‑145 (MQ=255)                                                                                        tttCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCgg                                          <  6:101146/145‑1 (MQ=255)                                                                                                                       aCCGATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTgg        >  6:18450/1‑148 (MQ=255)                                                                                                                          gATTCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAAc    >  3:51067/1‑149 (MQ=255)                                                                                                                            ttCTGGTGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAAccc  <  8:48250/149‑1 (MQ=255)                                                                                                                                  tGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTg         <  2:150472/136‑1 (MQ=255)                                                                                                                                  tGATTAAAAGCGGACGTAG‑CCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTg         >  1:150472/1‑136 (MQ=255)                                                                                                                                                     |                                                                                                                             ATGAATTGCTTGACTATCTGGCACGCGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACCGATTCTGGTGATTAAAAGCGGACGTAGCCCGGCGGCACAGCGACTGCTCAACACGACGGCAGGAATGGACCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCC  >  NC_000913/2720518‑2720790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑