Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,121,5860GC22.6% 63.0 / 18.0 31intergenic (‑32/+23)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (13/11);  new base C (4/3);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGC  >  NC_000913/2121449‑2121729
                                                                                                                                         |                                                                                                                                                
ccAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTg‑gg                                                                                                                                             <  4:84848/142‑1 (MQ=255)
ccAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTg‑gg                                                                                                                                             >  3:84848/1‑142 (MQ=255)
    ccATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGGAAggg                                                                                                                                      >  8:105636/1‑145 (MQ=255)
       tAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATcc                                                                                                                               <  7:105636/149‑1 (MQ=255)
         gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCC‑CACTGCGGGTAAggg                                                                                                                                      <  1:81075/140‑1 (MQ=255)
         gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCC‑CACTGCGGGTAAggg                                                                                                                                      >  2:81075/1‑140 (MQ=255)
          tCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGa                                                                                                                             >  4:16827/1‑148 (MQ=255)
                    atgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGtt                                                                                                                  <  3:16827/149‑1 (MQ=255)
                                  tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcag                                                                                                      >  5:41992/1‑147 (MQ=255)
                                  tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcag                                                                                                      >  3:94477/1‑147 (MQ=255)
                                  tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGGTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcag                                                                                                      >  7:7049/1‑147 (MQ=255)
                                    aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagca                                                                                                    >  1:99108/1‑147 (MQ=255)
                                        gCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttc                                                                                              <  2:99108/149‑1 (MQ=255)
                                                     gagaTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTc                                                                                 <  4:94477/149‑1 (MQ=255)
                                                                   aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGAt                                                                                                                                 <  4:116943/87‑1 (MQ=255)
                                                                   aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGca                                                                      <  5:145819/146‑1 (MQ=255)
                                                                   aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGca                                                                      >  6:145819/1‑146 (MQ=255)
                                                                              ctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                        >  2:4971/1‑149 (MQ=255)
                                                                                     gcgcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                      <  6:131315/112‑1 (MQ=255)
                                                                                     gcgcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                      >  5:131315/1‑112 (MQ=255)
                                                                                           ttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                             <  6:41992/147‑1 (MQ=255)
                                                                                           ttttAGATTTGTCATCGTTGTTCCTGTTAATAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTGCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                             <  8:7049/147‑1 (MQ=255)
                                                                                                     gTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCgg                                 >  5:96119/1‑149 (MQ=255)
                                                                                                               ttCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTg                       <  6:96119/149‑1 (MQ=255)
                                                                                                                     ttattaGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTTAGGCGCACCACCGGTTCGGTATTGGAGGTg                 >  5:117902/1‑149 (MQ=255)
                                                                                                                      ggatcttcccTTACCC‑CACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGa                     >  5:23985/8‑144 (MQ=255)
                                                                                                                      ggatcttcccTTACCC‑CACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGa                     <  6:23985/137‑1 (MQ=255)
                                                                                                                           ttcccTTACCC‑CACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg           >  7:21263/3‑149 (MQ=255)
                                                                                                                            tcccTTACCC‑CACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg                    >  1:151135/2‑139 (MQ=255)
                                                                                                                            tcccTTACCC‑CACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg                    <  2:151135/138‑1 (MQ=255)
                                                                                                                                     cGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCACCAGAGTTCGCCTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc  >  5:147301/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                
CCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTG‑GGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGC  >  NC_000913/2121449‑2121729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑