| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 437,513 | Δ1 bp | 6.7% | coding (623/975 nt) | yajO ← | 2‑carboxybenzaldehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 437,513 | 0 | A | . | 6.7% | 205.2 / 7.0 | 60 | coding (623/975 nt) | yajO | 2‑carboxybenzaldehyde reductase |
| Reads supporting (aligned to +/- strand): ref base A (23/33); new base . (2/2); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
CGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCA > NC_000913/437367‑437660 | cGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAc < 8:147204/148‑1 (MQ=255) ccTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCg < 6:314386/148‑1 (MQ=255) ccTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCg > 5:17002/1‑148 (MQ=255) tGTTAACCGCTCTGCGATTTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGc > 4:46234/1‑147 (MQ=255) ttAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 7:144768/146‑1 (MQ=255) ttAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCa < 6:267559/147‑1 (MQ=255) aCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGc < 1:137078/147‑1 (MQ=255) ctctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcctcc > 1:6461/1‑148 (MQ=255) gCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGa > 4:183473/1‑147 (MQ=255) gCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGa > 2:272654/1‑147 (MQ=255) cGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGa < 5:172483/146‑1 (MQ=255) cGATCTGCGCGTCATCTTCATCGCTTTCTTTATAGAGATTTATCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATa < 4:111972/148‑1 (MQ=255) tGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 3:46234/147‑1 (MQ=255) cgcgTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAac < 7:95158/143‑1 (MQ=255) gTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGt < 3:30756/148‑1 (MQ=255) ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcctc < 5:294225/128‑1 (MQ=255) ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcctc > 6:294225/1‑128 (MQ=255) tCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcctc < 4:239827/122‑1 (MQ=255) tCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcctc > 3:239827/1‑122 (MQ=255) ctttctttATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGa > 3:137702/1‑123 (MQ=255) ctttctttATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGa < 4:137702/123‑1 (MQ=255) tctttATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGctct < 2:135803/147‑1 (MQ=255) tctttATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGctc < 1:272654/146‑1 (MQ=255) taGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAg > 7:205243/1‑122 (MQ=255) taGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAg < 8:205243/122‑1 (MQ=255) gagaTTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGa > 5:244399/1‑148 (MQ=255) ccccACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAg < 4:56557/147‑1 (MQ=255) cTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATa > 7:239101/1‑99 (MQ=255) cTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATa < 8:239101/99‑1 (MQ=255) caGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATc > 7:126987/1‑148 (MQ=255) caGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATc > 6:155813/1‑148 (MQ=255) caGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATT‑CCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttct < 2:302396/121‑1 (MQ=255) caGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATT‑CCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttct < 2:297830/121‑1 (MQ=255) caGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATT‑CCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttct > 1:302396/1‑121 (MQ=255) caGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATT‑CCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttct > 1:297830/1‑121 (MQ=255) cacCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGTTTGTAGTGATCCTGc < 5:220434/148‑1 (MQ=255) ccAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCAt < 8:126987/148‑1 (MQ=255) cAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATa > 8:160469/1‑148 (MQ=255) cAGTCGTGCGGTAGTGTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATa < 4:48531/148‑1 (MQ=255) cGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTg < 6:244399/147‑1 (MQ=255) cGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGc < 6:17002/148‑1 (MQ=255) cGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGc > 1:253470/1‑148 (MQ=255) aCGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATc > 4:221027/1‑114 (MQ=255) aCGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGGAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATc < 3:221027/114‑1 (MQ=255) aCGCGTCAGACGGCCCCTTGCAAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGt > 5:106572/1‑148 (MQ=255) gTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGttttt < 5:155813/148‑1 (MQ=255) gTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGttttt < 3:183473/148‑1 (MQ=255) tCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTg < 7:160469/148‑1 (MQ=255) acgatatgCCCCTTGCCCGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATACCACAGTGGTATCCTCTCTCTCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGttttt < 5:87737/140‑1 (MQ=255) cTTGCAAGCTGTCTCCATGGAATTACCGCCAAGCCCTCCTGATAAAACAGTTGTAGCATCTCGAGCTCTTCTTCACGATAAATCAGATTGTAGTGTTCCTGCATACTGAAAAAGTGCGCCCAGCCGTGCTGTTTTTGGATTTCCAGTg < 5:257681/148‑1 (MQ=255) ttGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGcc > 1:205773/1‑124 (MQ=255) ttGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGcc < 2:205773/124‑1 (MQ=255) ttGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTcc < 1:14171/143‑1 (MQ=255) ttGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTcc > 2:14171/1‑143 (MQ=255) tGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCa > 3:154873/1‑143 (MQ=255) tGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCa < 4:154873/143‑1 (MQ=255) gCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGttt < 6:157714/123‑1 (MQ=255) gCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGttt > 5:157714/1‑123 (MQ=255) gAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGc > 4:101109/1‑148 (MQ=255) gAATTACCGCCACGCCATCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGc > 7:27610/1‑148 (MQ=255) aCCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCa > 3:161235/1‑148 (MQ=255) | CGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCA > NC_000913/437367‑437660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |