Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,480,199	Δ1 bp	6.2%	coding (1062/1758 nt)	ynbC →	putative esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,480,199	0	T	.	6.2%	216.4 / 7.0	64	coding (1062/1758 nt)	ynbC	putative esterase
Reads supporting (aligned to +/- strand):  ref base T (31/29);  new base . (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCT  >  NC_000913/1480056‑1480342                                                                                                                                                |                                                                                                                                                cTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATg                                                                                                                                             >  8:282619/1‑148 (MQ=255)           gtggACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCaaaa                                                                                                                                   <  8:287710/148‑1 (MQ=255)              gACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCTATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCa                                                                                                                                <  1:151168/148‑1 (MQ=255)               aCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCaa                                                                                                                               <  8:205821/148‑1 (MQ=255)                 ccGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCCCCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAAcc                                                                                                                             >  4:121101/1‑148 (MQ=255)                 ccGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAAcc                                                                                                                             >  1:277509/1‑148 (MQ=255)                          tATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCaaa                                                                                                                                    <  1:231425/132‑1 (MQ=255)                          tATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCaaa                                                                                                                                    >  2:231425/1‑132 (MQ=255)                           aTCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGgtag                                                                                                                    >  7:299156/1‑147 (MQ=255)                                cGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGc                                                                                                              <  7:127322/148‑1 (MQ=255)                                       tttAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCggg                                                                                                       >  8:209259/1‑148 (MQ=255)                                        ttAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGc                                                                                                      >  4:177820/1‑148 (MQ=255)                                           gCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCGAAATCAACCGCAAGGTAGTAACGCATTCGGGCGtt                                                                                                   <  7:301031/148‑1 (MQ=255)                                               aTCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGttt                                                                                                  >  1:326657/1‑145 (MQ=255)                                               aTCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGttt                                                                                                  <  2:326657/145‑1 (MQ=255)                                                 cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                                                                                            >  3:16023/1‑133 (MQ=255)                                                 cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                                                                                            <  4:16023/133‑1 (MQ=255)                                                 cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGA‑TATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                                                                                            >  5:97365/1‑132 (MQ=255)                                                 cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGA‑TATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                                                                                            >  5:93095/1‑132 (MQ=255)                                                 cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGA‑TATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                                                                                            <  6:93095/132‑1 (MQ=255)                                                 cGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGA‑TATGTCTATCAAAATCAACCGCAAGGTAGTAACGCAt                                                                                                            <  6:97365/132‑1 (MQ=255)                                                  gCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGa                                                                                            >  5:19685/1‑148 (MQ=255)                                                  gCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGa                                                                                            >  2:143944/1‑148 (MQ=255)                                                    tttATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAg                                                                                                                        >  5:174457/1‑118 (MQ=255)                                                       aTGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACaaa                                                                                        <  3:121101/147‑1 (MQ=255)                                                        tGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGTAAGGTAGTAACGCATTCGGGCGTTTAGTCGACaaaa                                                                                       <  8:53932/147‑1 (MQ=255)                                                          cGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCt                                                                                    <  6:119948/148‑1 (MQ=255)                                                           gAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTa                                                                                   <  5:267858/148‑1 (MQ=255)                                                             aaGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTAcc                                                                                 <  3:177820/148‑1 (MQ=255)                                                             aaGGCGATGAAATTGTGCGGGAAGCACTCTTCGGGCCTGCATCTCGGAAAGAGCACCGGCATTGATTCAGGCAGTTCGCTGGATTATTTATATCAAAATCAACAGCAACGTAGTAACGCAATCTGGCGTTTAGTCGACAAAATCTAcc                                                                                 <  1:105818/148‑1 (MQ=255)                                                                 cGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGaa                                                                             <  7:282619/148‑1 (MQ=255)                                                                    tGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAg                                                                          >  7:118624/1‑148 (MQ=255)                                                                      aaaTTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAgtg                                                                        >  6:263121/1‑148 (MQ=255)                                                                             tCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtggctg                                                                 >  4:136776/1‑148 (MQ=255)                                                                                     cACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTa                                                         >  1:228370/1‑148 (MQ=255)                                                                                      aCTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTAt                                                        <  1:143944/148‑1 (MQ=255)                                                                                        tctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCgg                                                                                                        <  8:208548/98‑1 (MQ=255)                                                                                        tctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCgg                                                                                                        >  7:208548/1‑98 (MQ=255)                                                                                         ctTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCg                                                     >  5:32866/1‑148 (MQ=255)                                                                                           tCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGcc                                                   >  1:115383/1‑148 (MQ=255)                                                                                                ccTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAAt                                                                                      >  3:65452/1‑108 (MQ=255)                                                                                                ccTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAAt                                                                                      <  4:65452/108‑1 (MQ=255)                                                                                                   gCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCaa                                            >  3:141085/1‑147 (MQ=255)                                                                                                   gCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCaa                                            >  7:233753/1‑147 (MQ=255)                                                                                                   gCATCTCGGAATGAGCACCGGCGTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGAGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCaa                                            >  7:287327/1‑147 (MQ=255)                                                                                                    cATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCaaa                                           <  4:141085/147‑1 (MQ=255)                                                                                                         cGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCAt                                     >  2:100155/1‑148 (MQ=255)                                                                                                          ggAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAAccc                                       <  8:299156/145‑1 (MQ=255)                                                                                                           gAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCAtt                                    >  1:326043/1‑147 (MQ=255)                                                                                                                    cctgCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACt                          <  2:277509/145‑1 (MQ=255)                                                                                                                    ccGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACt                          <  2:326043/148‑1 (MQ=255)                                                                                                                           tGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTa                     <  2:228370/146‑1 (MQ=255)                                                                                                                            gATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTaaa                   <  3:136776/147‑1 (MQ=255)                                                                                                                               tCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCCAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACaa                <  5:263121/147‑1 (MQ=255)                                                                                                                               tCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAg               >  4:121159/1‑148 (MQ=255)                                                                                                                                 aGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGc              <  6:19685/147‑1 (MQ=255)                                                                                                                                    cAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTa                                                                                                 >  1:226897/1‑61 (MQ=255)                                                                                                                                    cAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTa                                                                                                 <  2:226897/61‑1 (MQ=255)                                                                                                                                     aGTTCGCTGGATTATGTCTATCAAAATCAACCGCAATGTATTATCGCATTCGGTCGTTTAGTCGACAAAATCTACCTTAACAGTGTTGGCTGTCGCGGTATGCGCCAGCGAAAAACCCATTTACAAATACTGATTAAACAAGACGTTg         <  8:287327/148‑1 (MQ=255)                                                                                                                                       ttCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtgg                                                                    <  7:293417/87‑1 (MQ=255)                                                                                                                                       ttCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTtgg                                                                    >  8:293417/1‑87 (MQ=255)                                                                                                                                       ttCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGcc       >  2:218915/1‑148 (MQ=255)                                                                                                                                            tGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGAtct  >  1:65740/1‑148 (MQ=255)                                                                                                                                            tGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGAtct  >  1:114763/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                CTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCTCGGAATGAGCACCGGCTTTGATTCAGGCAGTTCGCTGGATTATGTCTATCAAAATCAACCGCAAGGTAGTAACGCATTCGGGCGTTTAGTCGACAAAATCTACCTGAACAGTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCT  >  NC_000913/1480056‑1480342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑