Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp 81.0% intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)46 (0.660) 40/254 NT 81.5% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 10 (0.150)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)39 (0.570) 35/252 NT 78.9% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 10 (0.150)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

GAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTA  >  NC_000913/382447‑382735
                                                                                                                                               |                                                                                                                                                 
gAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTa                                                                                                                                               <  3:238007/148‑1 (MQ=31)
    cTTGCAGAGGCTGTCGAGCATTATAACGAATGGCATACGCATAGTGCTCTGGGTTATAGCTCTGCACGGGAATATCTGCTGTAGCGGGCTGGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCtt                                                                                                                                           <  8:282257/148‑2 (MQ=11)
                ttCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTg                                                                                                                               >  8:161890/1‑148 (MQ=255)
                 tCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTgc                                                                                                                              >  2:214227/1‑148 (MQ=255)
                   gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGCGCAAATCCAATTACCTATCAGGCAGTTTgcg                                                                                                                             <  1:30933/147‑1 (MQ=255)
                    aGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTgcgc                                                                                                                            >  5:283778/1‑147 (MQ=255)
                             aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTAc                                                                                                                  >  7:203515/1‑148 (MQ=255)
                                         cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCACTTTCCGCCAATCACTACCGGACCATGaa                                                                                                       >  8:311887/1‑147 (MQ=255)
                                           cATAGTGCGCTGTTTTATCGCTCGCCACTGTAATATATGCGGCAGATGGCTTGTAATTGGGTAAGTGATAACAGATGTCTGGAAATATATTGGCAAATCCAATTACCTATCAGGCAGTTTGCGACAATCACTACCTGACCCTGAATaa                                                                                                    <  5:172900/148‑1 (MQ=18)
                                               gTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACgg                                                                                                 >  5:32275/1‑147 (MQ=255)
                                                    cTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATc                                                                                                                       <  2:88017/120‑1 (MQ=255)
                                                    cTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATc                                                                                                                       >  1:88017/1‑120 (MQ=255)
                                                         ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaa                                                                                      <  8:203515/148‑1 (MQ=255)
                                                           aTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa                                                                                     >  7:250404/1‑147 (MQ=255)
                                                              gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACg                                                                                 <  1:214227/148‑1 (MQ=255)
                                                              gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGACAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACg                                                                                 <  7:161890/148‑1 (MQ=255)
                                                                 cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACggg                                                                               >  7:210041/1‑147 (MQ=255)
                                                                   ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTAt                                                                            <  8:271101/148‑1 (MQ=255)
                                                                     aCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTc                                                                          >  4:31157/1‑148 (MQ=255)
                                                                      cGGGAATATCTGAGGCAGGGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTTGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGGCAATCACTACCGGACCCTGAATAACGGCACCATGACTTATACGGGTATTcg                                                                         <  6:292321/148‑1 (MQ=25)
                                                                        ggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTcgcg                                                                       >  5:108593/1‑148 (MQ=255)
                                                                             atCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                                                                   >  4:122866/1‑147 (MQ=255)
                                                                             atCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCAAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                                                                   >  7:302772/1‑147 (MQ=255)
                                                                                     cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACtt                                                          <  3:13063/148‑1 (MQ=255)
                                                                                     cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCACGACAAATACGGGTATTCGCGCCGATAACAACtt                                                          <  1:325505/148‑1 (MQ=255)
                                                                                          ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCt                                                     >  8:295188/1‑148 (MQ=255)
                                                                                                 aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGttt                                              >  7:278781/1‑148 (MQ=255)
                                                                                                   tGGGTTAAGTGATAACAGCTTTCAGGAAAGATAGGGGCAAATCCAATTACCTATCAGGCAGATTTCGCAAATCACTACCGGACCCTGAATAACGGCACCATGCCAAATACGGGTATTATCGCCGATAACAACTTCCCATCCAGttt                                              >  1:260057/1‑146 (MQ=37)
                                                                                                    gggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTtcat                                           >  6:163839/1‑148 (MQ=255)
                                                                                                       ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaa                                        >  4:54680/1‑148 (MQ=255)
                                                                                                       ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaa                                        <  3:54680/148‑1 (MQ=255)
                                                                                                           gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaataat                                    <  6:108593/148‑1 (MQ=255)
                                                                                                                aaCAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCt                               >  6:240174/1‑148 (MQ=255)
                                                                                                                            gAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCCCCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCt                   >  4:176844/1‑148 (MQ=255)
                                                                                                                            gAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCt                   >  6:9884/1‑148 (MQ=255)
                                                                                                                                tataGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAat               <  5:9884/148‑1 (MQ=255)
                                                                                                                                    gtcgCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATAtt            <  7:311887/144‑1 (MQ=255)
                                                                                                                                    ggggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCTCTACGTGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGattatt            <  6:32275/147‑1 (MQ=255)
                                                                                                                                     gggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATAtttt          <  3:31157/148‑1 (MQ=255)
                                                                                                                                      ggCAAATCCAATGTCCTATCAGGCAGTTTGCGCCAATTACTACCGGACCCTGAATAACGGCAACATGACAAATACGGGTATTCGCGCAGATAACAACTTCACCTGCAGTTTCATCAATACTTACCTGGTCTGAAATCTGAATAtttt          <  2:260057/147‑1 (MQ=255)
                                                                                                                                       gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTtc        >  2:283559/1‑148 (MQ=255)
                                                                                                                                         aaaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaata                                      <  1:126223/116‑1 (MQ=255)
                                                                                                                                         aaaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCaata                                      >  2:126223/1‑116 (MQ=255)
                                                                                                                                           aTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCtt    >  2:292408/1‑148 (MQ=255)
                                                                                                                                              cAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTa  <  1:283559/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTA  >  NC_000913/382447‑382735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.