Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,262,918 A→T 8.5% V248D (GTC→GAC)  tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,262,9180AT 8.5% 129.5 / 4.9 47V248D (GTC→GAC) tdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base A (24/17);  new base T (2/2);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

AGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACC  >  NC_000913/3262777‑3263064
                                                                                                                                             |                                                                                                                                                  
aGTTTCGAGAGTTAGTGACCTTCATGCCAGTATTTTTCAAGAACACGTAAATACTAAGAAAGACCTGAAATACCTAATAATCACTACTCTTTATTCACTACGCGTTCCAGGTCACCAAGGCTCTGTTTGGTTTGGCTGGCTACCCAgg                                                                                                                                              <  3:65426/148‑1 (MQ=255)
             cGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCaa                                                                                                                                  <  1:177276/147‑1 (MQ=255)
                    ttCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACa                                                                                                                          >  4:74549/1‑148 (MQ=255)
                     gcATGCCAGGCTTTTTACAGAACACGTAAATCCGAAGAAAGACCGCAAATACATAAAAATACCGCCTATTTATTCACTACGCGTTCCAGGTCACCCAGGCTATGGTAGGTTTGGCTGGCGACCCAGCACATCGACACAAAGTCGACAt                                                                                                                         >  4:82675/2‑148 (MQ=255)
                       aTGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCg                                                                                                                       >  3:149715/1‑148 (MQ=255)
                             ggCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTg                                                                                                                 >  7:6825/1‑148 (MQ=255)
                               cTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCg                                                                                                               >  7:210834/1‑148 (MQ=255)
                                  tttCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCggg                                                                                                             >  3:168224/1‑147 (MQ=255)
                                        gAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCcat                                                                                                      <  2:250432/148‑1 (MQ=255)
                                                  aTCCGAAGAAAGACCGGAAATACCTATTAATCCCGACTCTTTATTCACTTCGCGTTCCAGGTCCCCCAGGCTCTGGTTGGTTTGGCTGGCGCCCCAGGACAGCGGACCAACGTCGCCATGGCCACTGCGGGTACCCAGCATCAAGCCt                                                                                            >  4:227777/1‑148 (MQ=255)
                                                   tCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCtt                                                                                           >  5:144866/1‑148 (MQ=255)
                                                     cGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTcc                                                                                         >  8:158268/1‑148 (MQ=255)
                                                         gaaagaCCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAgcg                                                                                     <  8:6825/148‑1 (MQ=255)
                                                              aCCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTc                                                                                <  7:158268/148‑1 (MQ=255)
                                                                   aaaTACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCca                                                                                                       >  8:210842/1‑120 (MQ=255)
                                                                   aaaTACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCca                                                                                                       <  7:210842/120‑1 (MQ=255)
                                                                         cTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGa                                                                     <  2:68868/148‑1 (MQ=255)
                                                                          taataaTCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAg                                                                    >  6:252297/1‑148 (MQ=255)
                                                                           aataatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGa                                                                                                                            <  3:230606/91‑1 (MQ=255)
                                                                           aataatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGa                                                                                                                            >  4:230606/1‑91 (MQ=255)
                                                                            ataatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                     >  8:49670/1‑97 (MQ=255)
                                                                            ataatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                     <  7:49670/97‑1 (MQ=255)
                                                                            ataatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTAGGGTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGt                                                                  <  3:82675/148‑1 (MQ=255)
                                                                              aatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGTCCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                     >  6:20713/1‑95 (MQ=255)
                                                                              aatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGTCCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                     >  6:25166/1‑95 (MQ=255)
                                                                              aatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGTCCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                     <  5:25166/95‑1 (MQ=255)
                                                                              aatCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGTCCCAGGACATCGCACCAAAGTCGACATCGcc                                                                                                                     <  5:20713/95‑1 (MQ=255)
                                                                                taccGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTc                                                                                          <  8:132056/118‑1 (MQ=255)
                                                                                taccGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTc                                                                                          >  7:132056/3‑120 (MQ=255)
                                                                                          ttATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGAc                                                    >  1:293882/1‑148 (MQ=255)
                                                                                                aCTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTg                                               <  3:272211/147‑1 (MQ=255)
                                                                                                  tACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCg                                             >  8:268847/1‑147 (MQ=255)
                                                                                                      cgTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACcg                                        <  3:74549/148‑1 (MQ=255)
                                                                                                            aGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACcgcg                                      >  8:244969/1‑144 (MQ=255)
                                                                                                            aGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTCCCCGTTGCGAACcgcg                                      >  7:251139/1‑144 (MQ=255)
                                                                                                             ggTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGAt                                 >  1:195775/1‑148 (MQ=255)
                                                                                                               tCACCCAGGCTCTGGTTGGTTTGGCTGGCGCCCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGAtt                                >  1:58648/1‑147 (MQ=255)
                                                                                                                   ccAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGAcgc                           >  1:32443/1‑148 (MQ=255)
                                                                                                                    cAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGAcgcg                          <  4:149715/148‑1 (MQ=255)
                                                                                                                      ggCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCCCAGATTGACGCGcc                        <  7:268847/148‑1 (MQ=255)
                                                                                                                            ggttggttTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGc                   >  5:211299/1‑147 (MQ=255)
                                                                                                                             gttggttTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAac                                                            <  1:101754/105‑1 (MQ=255)
                                                                                                                             gttggttTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAac                                                            >  2:101754/1‑105 (MQ=255)
                                                                                                                             gttggttTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGcc                  >  3:268567/1‑147 (MQ=255)
                                                                                                                               tggttTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGcc                  >  1:286000/1‑145 (MQ=255)
                                                                                                                                           cGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAAc   >  2:224852/1‑148 (MQ=255)
                                                                                                                                            gACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAAcc  <  4:168224/148‑1 (MQ=255)
                                                                                                                                             aCCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCGAGATGCGCCACAAc   >  1:194002/1‑146 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
AGTTGCGCGCGTTCGTGACCTTCATGCCAGGCTTTTTCCAGAACACGTAAATCCGAAGAAAGACCGGAAATACCTAATAATCCCGACTCTTTATTCACTACGCGTTCCAGGTCACCCAGGCTCTGGTTGGTTTGGCTGGCGACCCAGGACATCGCACCAAAGTCGACATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACC  >  NC_000913/3262777‑3263064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑