Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,299,107	G→A	6.9%	G45D (GGC→GAC)	yhbO →	stress‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,107	0	G	A	6.9%	184.8 / 3.2	58	G45D (GGC→GAC)	yhbO	stress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base G (23/31);  new base A (2/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

AAGCATGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGT  >  NC_000913/3298970‑3299254                                                                                                                                          |                                                                                                                                                    aaGCATGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGga                                                                                                                                           <  8:75286/148‑1 (MQ=255) aaGCATGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGga                                                                                                                                           <  4:152567/148‑1 (MQ=255)  aGCATGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGgag                                                                                                                                          <  6:34913/148‑1 (MQ=255)    cATGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGgag                                                                                                                                          >  5:50561/1‑146 (MQ=255)      tGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGc                                                                                                                                      >  1:309779/1‑148 (MQ=255)           aaGAAAATTGCCGTTTTAATCACTGATGAAATTGAGGATTCAGAATTTACTTCACCAGCAGACGAGTTCCGTACAGCCGGACACGAAGAGATTACCATTGAAAAACAAGCGGGTAAAACGATGAAAGGCAAAAAAGGAGAAGCCAGCg                                                                                                                                 >  2:140541/1‑148 (MQ=255)            aGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACAATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGCAGCCAGCGt                                                                                                                                >  7:296865/1‑148 (MQ=255)             gAAAATTGTCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTg                                                                                                                               <  5:171183/148‑1 (MQ=255)                    gCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCAAGCGTGACCATc                                                                                                                         >  7:104313/1‑147 (MQ=255)                     ccGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCAt                                                                                                                          >  1:8169/1‑145 (MQ=255)                     ccGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCAt                                                                                                                          <  2:8169/145‑1 (MQ=255)                         tttAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATaa                                                                                                                    >  1:308866/1‑147 (MQ=255)                             aTCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCa                                                                                                               <  7:212147/148‑1 (MQ=255)                             aTCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGAGGTTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCa                                                                                                               <  7:221579/148‑1 (MQ=255)                              tCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCAt                                                                                                              >  5:106790/1‑148 (MQ=255)                                    atgaATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGa                                                                                                        <  1:227363/148‑1 (MQ=255)                                     tgaATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGa                                                                                                        <  2:309779/147‑1 (MQ=255)                                         tttGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGa                                                                                                   <  3:238615/148‑1 (MQ=255)                                           tGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGAc                                                                                                  <  2:308866/147‑1 (MQ=255)                                             aGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCg                                                                                                            >  6:190600/1‑135 (MQ=255)                                             aGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCg                                                                                                            <  5:190600/135‑1 (MQ=255)                                                aTTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGc                                                                                            <  6:50561/148‑1 (MQ=255)                                                  tCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCg                                                                                           >  4:35535/1‑147 (MQ=255)                                                       aTTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGttt                                                                                     >  4:181617/1‑148 (MQ=255)                                                        tttACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTg                                                                                    <  3:260942/148‑1 (MQ=255)                                                        tttACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTg                                                                                    <  3:265377/148‑1 (MQ=255)                                                         ttACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGa                                                                                   >  8:234907/1‑148 (MQ=255)                                                          tACTTCACCAGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGa                                                                                   <  6:128923/147‑1 (MQ=255)                                                           aCTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGAt                                                                                  <  5:224201/147‑1 (MQ=255)                                                                 cccGCAGACGAGGTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGGGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCctgc                                                                           <  2:93907/148‑1 (MQ=255)                                                                      aGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTa                                                                      <  3:181617/148‑1 (MQ=255)                                                                           aGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCggg                                                                 >  7:9313/1‑148 (MQ=255)                                                                                cGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGcc                                                            <  6:106790/148‑1 (MQ=255)                                                                                  tAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGACAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa                                                                                                       >  6:211697/1‑103 (MQ=255)                                                                                  tAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGACAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa                                                                                                       <  5:211697/103‑1 (MQ=255)                                                                                  tAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGACAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa                                                                                                       >  2:237756/1‑103 (MQ=255)                                                                                  tAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGACAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGaa                                                                                                       <  1:237756/103‑1 (MQ=255)                                                                                   aaaGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCAtt                                                         >  4:230541/1‑148 (MQ=255)                                                                                   aaaGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCAtt                                                         >  7:224995/1‑148 (MQ=255)                                                                                       ccGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCAcc                                                     <  3:35535/148‑1 (MQ=255)                                                                                          gACACGTAGGGATTACCATTGAAAAACATGCGGGTAAAACGTTGTATGGCAAAAAAGGAGAAGCCAGCGTGACCATCGTTAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGa                                                  <  1:140541/148‑1 (MQ=255)                                                                                               gAAGTGATTACCATTTAAAAACAAGCGGGTATAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCGATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATc                                             <  8:296865/148‑1 (MQ=255)                                                                                                   tGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCg                                         <  1:120518/148‑1 (MQ=255)                                                                                                    gATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGCCGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGt                                        >  4:284113/1‑148 (MQ=255)                                                                                                    gATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGt                                        >  5:286827/1‑148 (MQ=255)                                                                                                     aTTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTg                                       <  6:286827/148‑1 (MQ=255)                                                                                                           aTTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGAc                                  >  3:295904/1‑147 (MQ=255)                                                                                                                aaaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGt                            >  3:48881/1‑148 (MQ=255)                                                                                                                 aaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCAcc                                                     <  4:17712/122‑1 (MQ=255)                                                                                                                 aaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCAcc                                                     >  3:17712/1‑122 (MQ=255)                                                                                                                 aaaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGtt                           <  8:9313/148‑1 (MQ=255)                                                                                                                  aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGttt                          <  8:224995/148‑1 (MQ=255)                                                                                                                  aaCAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGttt                          <  7:234907/148‑1 (MQ=255)                                                                                                                         gggTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCAcc                   >  7:61660/1‑148 (MQ=255)                                                                                                                            tAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCttt                <  8:61660/148‑1 (MQ=255)                                                                                                                                       aaGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGAttt     >  4:135450/1‑148 (MQ=255)                                                                                                                                        aGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCa                     <  2:240851/131‑1 (MQ=255)                                                                                                                                        aGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCa                     >  1:240851/1‑131 (MQ=255)                                                                                                                                          gCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTtgt  <  8:104313/148‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                    AAGCATGAGTAAGAAAATTGCCGTTTTAATCACTGATGAATTTGAGGATTCAGAATTTACTTCACCCGCAGACGAGTTCCGTAAAGCCGGACACGAAGTGATTACCATTGAAAAACAAGCGGGTAAAACGGTGAAAGGCAAAAAAGGAGAAGCCAGCGTGACCATCGATAAATCCATCGATGAAGTGACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGT  >  NC_000913/3298970‑3299254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑