Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,973,744 T→A 5.4% I46N (ATC→AAC)  rffH → glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,973,7440TA 5.4% 234.6 / 3.7 74I46N (ATC→AAC) rffHglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (33/37);  new base A (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

GAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGCCTTTATCATCGGTGAAA  >  NC_000913/3973599‑3973890
                                                                                                                                                 |                                                                                                                                                  
gAGGCATACATGAAAGGTATTATCCTGGCGGGCGGGTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGTAGCAACTGTTGCAGATTTACGATAAGCCTATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATcc                                                                                                                                                  <  1:288907/148‑1 (MQ=255)
    cAAACATGAAAGGTATTATCCTGGCGGGCCGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGa                                                                                                                                              <  8:16753/148‑1 (MQ=255)
    cAAACATGAAAGGTATTATCCTGCCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGAAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCAGCTGTCGGTGCGGATGCTCGCCGGTATCcgcg                                                                                                                                               >  6:82927/1‑147 (MQ=255)
     aaaCATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGaa                                                                                                                                             <  8:226293/148‑1 (MQ=255)
        cATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAAtt                                                                                                                                          >  4:157040/1‑148 (MQ=255)
            aaaGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCtca                                                                                                                                      <  1:93249/148‑1 (MQ=255)
                 tattatCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCtcatcatc                                                                                                                                 <  2:297370/148‑1 (MQ=255)
                  attatCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCtcatcatca                                                                                                                                >  6:95993/1‑148 (MQ=255)
                   ttatCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCtcatcatca                                                                                                                                <  8:287999/147‑1 (MQ=255)
                     atCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAAtt                                                                                                                                          >  6:197979/1‑135 (MQ=255)
                     atCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAAtt                                                                                                                                          <  5:197979/135‑1 (MQ=255)
                     atCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACt                                                                                                                              <  5:95993/147‑1 (MQ=255)
                                  gTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATaa                                                                                                                <  2:184230/148‑1 (MQ=255)
                                       ggCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCtcatcatc                                                                                                                                 >  7:119825/1‑126 (MQ=255)
                                       ggCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCtcatcatc                                                                                                                                 <  8:119825/126‑1 (MQ=255)
                                        gCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTa                                                                                                          <  3:157040/148‑1 (MQ=255)
                                          aCCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTAtt                                                                                                        >  3:294126/1‑148 (MQ=255)
                                            ccGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTc                                                                                                      >  2:251254/1‑148 (MQ=255)
                                             cGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTAACCGCGAAATTCTCATCATCACt                                                                                                                              >  6:23848/1‑123 (MQ=255)
                                             cGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTAACCGCGAAATTCTCATCATCACt                                                                                                                              <  5:19443/123‑1 (MQ=255)
                                             cGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTAACCGCGAAATTCTCATCATCACt                                                                                                                              <  5:23848/123‑1 (MQ=255)
                                             cGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTAACCGCGAAATTCTCATCATCACt                                                                                                                              >  6:19443/1‑123 (MQ=255)
                                              gATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCa                                                                                                    <  1:137562/148‑1 (MQ=255)
                                                     tCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCctg                                                                                             >  3:225242/1‑148 (MQ=255)
                                                        gATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCctgctg                                                                                          >  7:252418/1‑148 (MQ=255)
                                                          ttACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTggg                                                                                        >  2:122605/1‑148 (MQ=255)
                                                               cgcgGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATg                                                                                   <  3:1941/148‑1 (MQ=255)
                                                                 cggcgTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGt                                                                                 <  4:80135/148‑1 (MQ=255)
                                                                   gcgTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAg                                                                               <  1:200544/148‑1 (MQ=255)
                                                                   gcgTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAg                                                                               <  6:224021/148‑1 (MQ=255)
                                                                    cgTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGt                                                                              <  8:252418/148‑1 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTa                                                                                >  4:158821/1‑143 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTa                                                                                >  8:234645/1‑143 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTa                                                                                <  7:234645/143‑1 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTa                                                                                <  3:158821/143‑1 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTg                                                                             <  4:225242/146‑1 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAg                                                                           >  4:101782/1‑148 (MQ=255)
                                                                       aTCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAg                                                                           >  8:27318/1‑148 (MQ=255)
                                                                          gAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTc                                                                        <  4:144305/148‑1 (MQ=255)
                                                                            aGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCgg                                                                      >  4:281481/1‑148 (MQ=255)
                                                                            aGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCgg                                                                      <  5:162262/148‑1 (MQ=255)
                                                                                aCTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTg                                                                             <  2:315541/137‑1 (MQ=255)
                                                                                aCTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTg                                                                             >  1:315541/1‑137 (MQ=255)
                                                                                 cTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATc                                                                  >  7:142741/1‑147 (MQ=255)
                                                                                   gTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGt                                                                                 >  6:73678/1‑130 (MQ=255)
                                                                                   gTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGt                                                                                 <  5:73678/130‑1 (MQ=255)
                                                                                   gTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAg                                                               >  6:8416/1‑148 (MQ=255)
                                                                                          aTTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAat                                                        <  1:251254/148‑1 (MQ=255)
                                                                                          aTTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAat                                                        <  8:142741/148‑1 (MQ=255)
                                                                                              aCGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATg                                                     <  1:122605/147‑1 (MQ=255)
                                                                                                 aTAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAg                                                                               >  1:136397/1‑118 (MQ=255)
                                                                                                 aTAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAg                                                                               <  2:136397/118‑1 (MQ=255)
                                                                                                      ccAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAAcagc                                            >  5:220828/1‑148 (MQ=255)
                                                                                                          tGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTa                                                                                                                             <  8:210504/63‑1 (MQ=255)
                                                                                                          tGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTa                                                                                                                             >  7:210504/1‑63 (MQ=255)
                                                                                                              ttACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCg                                    >  1:276112/1‑148 (MQ=255)
                                                                                                                aCTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCgg                                   <  4:250467/147‑1 (MQ=255)
                                                                                                                       gCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTg                           <  4:294126/148‑1 (MQ=255)
                                                                                                                          gTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGgcg                        >  5:116396/1‑148 (MQ=255)
                                                                                                                           tCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGgcgc                       <  7:27318/148‑1 (MQ=255)
                                                                                                                           tCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCGCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGgcgc                       <  3:101782/148‑1 (MQ=255)
                                                                                                                               tGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGc                   >  7:327219/1‑148 (MQ=255)
                                                                                                                                gCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGcc                  >  8:31568/1‑148 (MQ=255)
                                                                                                                                 cTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGCCt                 >  2:121722/1‑148 (MQ=255)
                                                                                                                                   gATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGCCtt                >  2:182293/1‑147 (MQ=255)
                                                                                                                                   gATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATCGAAGAGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGCCGCAGGCCtt                >  2:265031/1‑147 (MQ=255)
                                                                                                                                      gCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGgc                         >  2:18686/1‑135 (MQ=255)
                                                                                                                                      gCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGgc                         <  1:18686/135‑1 (MQ=255)
                                                                                                                                       cTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGCCTTTatc            <  1:121722/147‑1 (MQ=255)
                                                                                                                                         ggCCGATATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCgg                                   >  3:241531/1‑122 (MQ=255)
                                                                                                                                         ggCCGATATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCgg                                   <  4:241531/122‑1 (MQ=255)
                                                                                                                                                aTCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAat                                                        >  6:42834/1‑94 (MQ=255)
                                                                                                                                                aTCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAat                                                        <  5:42834/94‑1 (MQ=255)
                                                                                                                                                aTCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGCCTTTATCATCGGTGaaa  >  5:275221/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGCACCCGATTGCATCCGATTACGCGCGGCGTATCGAAGCAACTGTTGCCGATTTACGATAAGCCAATGATTTACTATCCGCTGTCGGTGCTGATGCTGGCCGGTATCCGCGAAATTCTCATCATCACTACGCCGGAAGATAAAGGTTATTTCCAGCGCCTGCTGGGCGATGGTAGTGAGTTCGGTATCCAGCTGGAATATGCCGAACAGCCCAGCCCGGACGGTCTGGCGCAGGCCTTTATCATCGGTGAAA  >  NC_000913/3973599‑3973890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑