Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,344,201	C→G	6.2%	A278G (GCT→GGT)	melB →	melibiose:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,344,201	0	C	G	6.2%	227.0 / 5.3	65	A278G (GCT→GGT)	melB	melibiose:sodium symporter
Reads supporting (aligned to +/- strand):  ref base C (24/37);  new base G (2/2);  total (26/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGT  >  NC_000913/4344054‑4344342                                                                                                                                                    |                                                                                                                                              aaaaCGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAgctgc                                                                                                                                               <  1:94131/148‑1 (MQ=255)  aaaCGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAgctgct                                                                                                                                              <  2:91463/148‑1 (MQ=255)   aaCGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAgctgct                                                                                                                                              >  2:50895/1‑147 (MQ=255)    aCGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTaa                                                                                                                                            <  1:50895/148‑1 (MQ=255)    aCGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTaa                                                                                                                                            <  6:77908/148‑1 (MQ=255)    aCGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTaa                                                                                                                                            <  5:93273/148‑1 (MQ=255)       aTCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGGCGTATGCGGGAGCTGCTAACCt                                                                                                                                         <  2:116804/148‑1 (MQ=255)           gCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTg                                                                                                                                     <  7:194140/148‑1 (MQ=255)               tCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTaa                                                                                                                                            >  1:278573/1‑137 (MQ=255)               tCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTaa                                                                                                                                            <  2:278573/137‑1 (MQ=255)                  tGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAg                                                                                                                              <  2:188223/148‑1 (MQ=255)                    ccTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTa                                                                                                                            >  7:194653/1‑148 (MQ=255)                    actctTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTa                                                                                                                            >  2:69822/2‑148 (MQ=255)                          gggTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTAttc                                                                                                                         <  3:104951/145‑1 (MQ=255)                          gggTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTAttc                                                                                                                         <  4:8592/145‑1 (MQ=255)                            gTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTccc                                                                                                                    <  2:94289/148‑1 (MQ=255)                                ggCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGc                                                                                                                >  7:16550/1‑148 (MQ=255)                                ggCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGc                                                                                                                >  2:62505/1‑148 (MQ=255)                                 gCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGGTAACCTGGTGACGTTAGTATTCTTCCCACGCt                                                                                                               >  6:48483/1‑148 (MQ=255)                                  ctctTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCt                                                                                                               >  5:221209/1‑147 (MQ=255)                                    ctTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGGTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAg                                                                                                            >  2:163702/1‑148 (MQ=255)                                           ataATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAAt                                                                                                      >  3:163184/1‑147 (MQ=255)                                              aTGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGGTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCAt                                                                                                   <  5:48483/147‑1 (MQ=255)                                                  aGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGtt                                                                                                                                >  5:9374/1‑114 (MQ=255)                                                  aGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGtt                                                                                                                                <  6:9374/114‑1 (MQ=255)                                                       gCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTaaa                                                                                                       <  8:159066/134‑1 (MQ=255)                                                       gCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTaaa                                                                                                       >  7:159066/1‑134 (MQ=255)                                                        cAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACg                                                                                         >  3:204905/1‑147 (MQ=255)                                                           cATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGGTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCAtt                                                                                     <  1:163702/148‑1 (MQ=255)                                                           cATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCAtt                                                                                     >  1:69490/1‑148 (MQ=255)                                                             ttattaCCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCAtttt                                                                                   <  4:204905/148‑1 (MQ=255)                                                                ttcccGGCGTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTAGCTGACGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTAt                                                                                 <  6:151527/144‑1 (MQ=255)                                                                 taCCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATgg                                                                               >  2:231777/1‑148 (MQ=255)                                                                      gCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCgg                                                                          <  8:194653/148‑1 (MQ=255)                                                                      gCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCg                                                                           >  3:135679/1‑147 (MQ=255)                                                                          ttctatctatTATTTCTCCTATGTTATCGGTGATGCGGACTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGc                                                                       <  2:252712/145‑1 (MQ=255)                                                                              atctatTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTa                                                                  >  3:48041/1‑148 (MQ=255)                                                                               tctatTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCt                                                                   <  6:221209/146‑1 (MQ=255)                                                                               tctatTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTAt                                                                 >  5:93672/1‑148 (MQ=255)                                                                                  attattTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAg                                                                                                                              <  3:251671/84‑1 (MQ=255)                                                                                  attattTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAg                                                                                                                              >  4:251671/1‑84 (MQ=255)                                                                                  attattTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCAt                                                                     >  2:40823/1‑141 (MQ=255)                                                                                  attattTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCAt                                                                     <  1:40823/141‑1 (MQ=255)                                                                                   ttattTCTTATATGTTATCGGTGATGCGTATTTGTTCCCCTTTTATCTGTCTTATGCGGTAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTAtt                                                                <  8:16550/145‑1 (MQ=255)                                                                                   ttattTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTAtt                                                                <  1:69822/145‑1 (MQ=255)                                                                                   ttattTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGCAGCATCTAttctt                                                             <  8:174945/148‑1 (MQ=255)                                                                                      tttCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTcc                                                           <  2:69490/147‑1 (MQ=255)                                                                                            atatGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGgtgt                                                      <  1:62505/146‑1 (MQ=255)                                                                                            atatGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGtt                                                     <  7:278997/147‑1 (MQ=255)                                                                                                   aTCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCtgtg                                             >  6:124304/1‑148 (MQ=255)                                                                                                      ggTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTgtggtg                                          <  4:48041/148‑1 (MQ=255)                                                                                                       gTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTgtgg                                            >  2:311965/1‑145 (MQ=255)                                                                                                       gTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTgtgg                                            <  1:311965/145‑1 (MQ=255)                                                                                                           tGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTtctc                                     <  4:163184/148‑1 (MQ=255)                                                                                                             cGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCAtttt                                                                                   <  7:81524/100‑1 (MQ=255)                                                                                                             cGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCAtttt                                                                                   >  8:81524/1‑100 (MQ=255)                                                                                                             cGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCt                                   <  3:291499/148‑1 (MQ=255)                                                                                                              ggATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTg                                  <  6:93672/148‑1 (MQ=255)                                                                                                              ggATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTg                                  <  4:135679/148‑1 (MQ=255)                                                                                                                 tttGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTa                               <  5:124304/148‑1 (MQ=255)                                                                                                                 tttGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTa                               <  1:231777/148‑1 (MQ=255)                                                                                                                  ttGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTaa                              >  8:141959/1‑148 (MQ=255)                                                                                                                        cccTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGgtgt                                         >  6:101864/1‑131 (MQ=255)                                                                                                                        cccTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGgtgt                                         <  5:101864/131‑1 (MQ=255)                                                                                                                                         tGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACaa        <  7:141959/147‑1 (MQ=255)                                                                                                                                           cGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCg                                                          <  6:271435/95‑1 (MQ=255)                                                                                                                                           cGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCg                                                          >  5:271435/1‑95 (MQ=255)                                                                                                                                              gAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAAcgtcgt  >  2:223424/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                              AAAACGATCAGCTTTCATGCCTCTTGGGTATGGCTCTTGCTTATAATGTAGCCAGCAACATTATTACCGGCTTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTTGTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTATCCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGCTATCACAACGTCGT  >  NC_000913/4344054‑4344342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑