Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 768,922 G→A 7.4% T315T (ACG→ACA)  mngB → alpha‑mannosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913768,9220GA 7.4% 170.2 / 3.5 55T315T (ACG→ACA) mngBalpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base A (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

ACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACAT  >  NC_000913/768776‑769065
                                                                                                                                                  |                                                                                                                                               
aCCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCGAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGt                                                                                                                                                >  6:37840/1‑148 (MQ=255)
 cccTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGtt                                                                                                                                               <  4:151927/148‑1 (MQ=255)
        aGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGTTTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGttt                                                                                                                                         <  1:38759/147‑1 (MQ=255)
        aGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGttt                                                                                                                                         >  2:38759/1‑147 (MQ=255)
               tttATTGATTGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATac                                                                                                                                  >  8:317827/1‑147 (MQ=255)
               tttATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTAAGGATATAAAAAATGCCAACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGAAACCGCTGGCAACACATCCCTGGACCTTGGCTTTTGAATaa                                                                                                                                  >  8:322233/1‑146 (MQ=255)
                       tGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCGAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGc                                                                                                                         <  5:37840/148‑1 (MQ=255)
                           aaaTATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGc                                                                                                                     <  3:137470/148‑1 (MQ=255)
                            aaTATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCt                                                                                                                    <  6:78365/148‑1 (MQ=255)
                                    cgcgTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaaa                                                                                                             <  7:322233/147‑1 (MQ=255)
                                    cgcgTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaaa                                                                                                             <  7:317827/147‑1 (MQ=255)
                                      cgTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAAtg                                                                                                          >  4:148626/1‑148 (MQ=255)
                                         gCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAAtgtg                                                                                                        >  3:170449/1‑147 (MQ=255)
                                          cATCTCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGTGAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTgg                                                                                                       <  6:197263/147‑1 (MQ=255)
                                            tCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGTAAATGTGGa                                                                                                      >  8:247991/1‑146 (MQ=255)
                                             cGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaaa                                                                                                             >  7:117267/1‑138 (MQ=255)
                                             cGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGaaa                                                                                                             <  8:117267/138‑1 (MQ=255)
                                                aCCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAgaga                                                                                                <  3:148626/148‑1 (MQ=255)
                                                aCCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAgaga                                                                                                <  5:217406/148‑1 (MQ=255)
                                                     cGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTTAAAATAAGATTGTTAATCTGCTGGGACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCtt                                                                                            >  2:192517/1‑147 (MQ=255)
                                                            aCGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAAtg                                                                                                          <  3:284984/126‑1 (MQ=255)
                                                            aCGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAAtg                                                                                                          >  4:284984/1‑126 (MQ=255)
                                                            aCGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCAAGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAAt                                                                                     >  2:272697/1‑147 (MQ=255)
                                                             cgcgTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGGACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTaaaaaa                                                                                      <  1:192517/145‑1 (MQ=255)
                                                             cgcgTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATc                                                                                    <  4:170449/147‑1 (MQ=255)
                                                                    ggATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCa                                                                             >  4:167403/1‑147 (MQ=255)
                                                                    ggATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCa                                                                             <  3:167403/147‑1 (MQ=255)
                                                                          cAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAg                                                                       >  5:139541/1‑147 (MQ=255)
                                                                            aaaTTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTAt                                                                    >  5:243537/1‑148 (MQ=255)
                                                                              aTTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCg                                                                  <  4:139406/148‑1 (MQ=255)
                                                                                               tGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgctgctgct                                                         <  8:203046/140‑1 (MQ=255)
                                                                                               tGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgctgctgct                                                         >  7:203046/1‑140 (MQ=255)
                                                                                               tGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGATTTGAATACCACCACGGCTTGCTGGAGAAAAAGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGAATCTGCTGTAGTGa                                                  >  6:157966/1‑147 (MQ=255)
                                                                                                gAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGCAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTATTGAc                                                 >  6:243898/1‑147 (MQ=255)
                                                                                                    aTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACATTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGAc                                                                         >  1:171915/1‑119 (MQ=255)
                                                                                                    aTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACATTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGAc                                                                         >  1:166906/1‑119 (MQ=255)
                                                                                                    aTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACATTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGAc                                                                         <  2:166906/119‑1 (MQ=255)
                                                                                                    aTAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACATTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGAc                                                                         <  2:171915/119‑1 (MQ=255)
                                                                                                     tAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgct                                                               <  7:219755/128‑1 (MQ=255)
                                                                                                     tAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGgct                                                               >  8:219755/1‑128 (MQ=255)
                                                                                                       aGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTc                                          <  6:139541/147‑1 (MQ=255)
                                                                                                                  ctgctgGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAAtcg                              >  5:123011/1‑148 (MQ=255)
                                                                                                                     ctgGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAAtcgt                             <  6:243537/146‑1 (MQ=255)
                                                                                                                            cGCTGGCAACACTGGGCTGGACGTTGGGTTTTGAATACCACCACGGCTTGGTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCtt                    <  5:157966/148‑1 (MQ=255)
                                                                                                                                  cAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACt              >  1:99791/1‑148 (MQ=255)
                                                                                                                                     cacTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGt                                                       <  7:167905/104‑1 (MQ=255)
                                                                                                                                     cacTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGt                                                       >  8:167905/1‑104 (MQ=255)
                                                                                                                                      acTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCt          <  1:272697/148‑1 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGctgctgctg                                                        >  4:111950/1‑94 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGctgctgctg                                                        <  3:111950/94‑1 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGaa                <  6:206536/134‑1 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGaa                >  5:206536/1‑134 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACAt  >  3:111984/1‑148 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACAt  >  2:271804/1‑148 (MQ=255)
                                                                                                                                              ggACGTTGGGTTTTGAATACCACAACGGCTTGCTGGAGAAAATGTGGAAAGAGATCATAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGATACAAAGTTCATCGCGAAATCGTCGCCCGCTTCCAACAGTCTGAAGAc    >  4:197027/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACAT  >  NC_000913/768776‑769065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑