Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 836,040 C→T 5.7% L265L (CTG→TTG)  ybiB → putative family 3 glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913836,0400CT 5.7% 233.8 / 2.6 70L265L (CTG→TTG) ybiBputative family 3 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (36/30);  new base T (2/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTATCAGCGACGGCCT  >  NC_000913/835895‑836185
                                                                                                                                                 |                                                                                                                                                 
cGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTg                                                                                                                                                 <  5:152647/148‑1 (MQ=255)
                      ctaATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGaa                                                                                                                            <  3:102936/147‑1 (MQ=255)
                          tGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCAc                                                                                                                       <  7:247985/148‑1 (MQ=255)
                           gCATTGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGAGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGAAAAAGATCCGGAAACCaag                                                                                                                      >  2:269675/1‑146 (MQ=255)
                           gCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACg                                                                                                                      >  7:38624/1‑148 (MQ=255)
                               ggTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGgcg                                                                                                                   >  1:24814/1‑147 (MQ=255)
                               ggTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGgcg                                                                                                                   >  8:301876/1‑147 (MQ=255)
                                 tACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAg                                                                                                                >  5:308739/1‑148 (MQ=255)
                                     gAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGa                                                                                                            >  7:219697/1‑148 (MQ=255)
                                      aaGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTgg                                                                                                             <  8:230150/146‑1 (MQ=255)
                                         gTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACGGTGAAGGGATGCGTGTGCTGTATGAAAAACAGGTCACTGCTGTTAGGGAGTTACTGCGACAAGCAATAGTTCCGGAAACCACGGCGCAGTGGAtttt                                                                                                        <  5:64393/148‑3 (MQ=255)
                                         gTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTATTGCcaca                                                                                                                                            <  2:170271/112‑1 (MQ=255)
                                         gTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTATTGCcaca                                                                                                                                            >  1:165166/1‑112 (MQ=255)
                                         gTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTATTGCcaca                                                                                                                                            <  2:165166/112‑1 (MQ=255)
                                         gTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTATTGCcaca                                                                                                                                            >  1:170271/1‑112 (MQ=255)
                                          tGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAg                                                                                                       <  7:301876/148‑1 (MQ=255)
                                               tgtATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTg                                                                                                  <  6:308739/148‑1 (MQ=255)
                                                  aTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCt                                                                                               >  2:242858/1‑148 (MQ=255)
                                                    gCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTg                                                                                             >  6:169928/1‑148 (MQ=255)
                                                     cTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGc                                                                                            <  8:38624/148‑1 (MQ=255)
                                                       aaTCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTg                                                                                          >  3:112536/1‑148 (MQ=255)
                                                       aaTCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTg                                                                                          >  3:154333/1‑148 (MQ=255)
                                                       aaTCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTg                                                                                          >  1:278942/1‑148 (MQ=255)
                                                        aTCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTgg                                                                                         <  8:219697/148‑1 (MQ=255)
                                                        aTCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTgg                                                                                         >  4:168812/1‑148 (MQ=255)
                                                         tCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATACGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTgg                                                                                         >  1:237683/1‑147 (MQ=255)
                                                          ccGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCgg                                                                                                                              >  3:147609/1‑109 (MQ=255)
                                                          ccGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCgg                                                                                                                              <  4:147609/109‑1 (MQ=255)
                                                               gcgcTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGaa                                                                                  <  5:169928/148‑1 (MQ=255)
                                                                  cTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCg                                                                               <  1:242858/148‑1 (MQ=255)
                                                                     cccGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGAt                                                                             <  2:24814/147‑1 (MQ=255)
                                                                             tCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATc                                                                    >  7:280835/1‑148 (MQ=255)
                                                                                  ctcATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCCCGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTg                                                                >  2:262696/1‑147 (MQ=255)
                                                                                  ctcATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTg                                                                >  6:38129/1‑147 (MQ=255)
                                                                                      ttGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAAt                                                           >  4:262486/1‑148 (MQ=255)
                                                                                      ttGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAAt                                                           >  2:139727/1‑148 (MQ=255)
                                                                                          ccGTTAAGGGATGCGGGTGCTGTATGAAAAACAGGACTCTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGACTGAGCGTTGCCTTGCTGTCAGCGAACCGATTCCCGAATCGCTGTAAATCCAg                                                       <  1:269675/148‑1 (MQ=255)
                                                                                           cGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGa                                                      <  1:139727/148‑1 (MQ=255)
                                                                                           cGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGa                                                      <  3:262486/148‑1 (MQ=255)
                                                                                            gTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         >  7:65253/1‑144 (MQ=255)
                                                                                            gTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         <  8:65253/144‑1 (MQ=255)
                                                                                               aaGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         <  8:169711/141‑1 (MQ=255)
                                                                                               aaGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         >  7:169711/1‑141 (MQ=255)
                                                                                                   gATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         <  3:262564/137‑1 (MQ=255)
                                                                                                   gATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         >  4:262564/1‑137 (MQ=255)
                                                                                                      gCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTtgctgc                                           >  4:110869/1‑148 (MQ=255)
                                                                                                        gggTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCt                                         <  2:278942/148‑1 (MQ=255)
                                                                                                            gCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         <  5:88894/128‑1 (MQ=255)
                                                                                                            gCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATcc                                                         >  6:88894/1‑128 (MQ=255)
                                                                                                              tGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGc                                                  >  3:277662/1‑133 (MQ=255)
                                                                                                              tGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGc                                                  <  4:277662/133‑1 (MQ=255)
                                                                                                                 aTGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTAc                                >  4:251608/1‑148 (MQ=255)
                                                                                                                 aTGAAAAACAGGACACTGCTGGTAGCCAGTTACTGCCACAAGCAAAAGATCCCGAAACCACGGCCCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGTCTTTCTGCCTGGTGGCTAc                                >  2:250297/1‑148 (MQ=255)
                                                                                                                  tGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACg                               >  8:154703/1‑148 (MQ=255)
                                                                                                                     aaaaCAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGt                            >  4:173974/1‑148 (MQ=255)
                                                                                                                              cacTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGAAGGCTTGCTGCCTGGGGGCTACGGGTGAAGCGGCa                   <  1:262696/148‑1 (MQ=255)
                                                                                                                                ctgctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGa                                                      >  7:334113/1‑111 (MQ=255)
                                                                                                                                ctgctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGa                                                      <  8:334113/111‑1 (MQ=255)
                                                                                                                                 tgctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGc                                                                  >  7:19310/1‑98 (MQ=255)
                                                                                                                                 tgctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGc                                                                  <  8:19310/98‑1 (MQ=255)
                                                                                                                                 tgctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCa                                                        <  7:202180/108‑1 (MQ=255)
                                                                                                                                 tgctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCa                                                        >  8:202180/1‑108 (MQ=255)
                                                                                                                                  gctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACt                >  2:22636/1‑147 (MQ=255)
                                                                                                                                  gctgGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTa               >  7:47750/1‑148 (MQ=255)
                                                                                                                                      gTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTATc             <  5:38129/146‑1 (MQ=255)
                                                                                                                                      gTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTATCa            <  1:22636/147‑1 (MQ=255)
                                                                                                                                      gTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTATCAg           <  7:154703/148‑1 (MQ=255)
                                                                                                                                               tACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCtt                                                >  5:201218/1‑102 (MQ=255)
                                                                                                                                               tACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCtt                                                <  6:201218/102‑1 (MQ=255)
                                                                                                                                               tACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTATCAGCGACGGCCt  >  4:20581/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTTGCCTTGCTGGCAGCGAACCGATTCCCGAATCGCTGAAAATCCAGATGGCTTGCTGCCTGGTGGCTACGGGTGAAGCGGCAACTATCAGCGACGGCCT  >  NC_000913/835895‑836185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: