Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 897,503 G→A 6.3% M140I (ATG→ATA potI → putrescine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913897,5030GA 6.3% 208.4 / 3.6 63M140I (ATG→ATApotIputrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (26/33);  new base A (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCAT  >  NC_000913/897365‑897646
                                                                                                                                          |                                                                                                                                               
cAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGGCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATc                                                                                                                                        <  2:151049/148‑1 (MQ=255)
        gcgGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATctggctgg                                                                                                                                 <  2:104800/147‑1 (MQ=255)
                  tGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATct                                                                                                                                       >  7:141458/1‑131 (MQ=255)
                  tGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATct                                                                                                                                       <  8:141458/131‑1 (MQ=255)
                    gCTTTTCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACttt                                                                                                                    <  2:116559/148‑1 (MQ=255)
                     cTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACtttt                                                                                                                   <  7:274078/148‑1 (MQ=255)
                        tGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTg                                                                                                                 <  8:79337/147‑1 (MQ=255)
                           cTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACg                                                                                                             >  4:292921/1‑148 (MQ=255)
                                   tCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTAAGGCTTAtgt                                                                                                     >  5:250979/1‑148 (MQ=255)
                                    cACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACttttt                                                                                                                  <  6:262976/134‑1 (MQ=255)
                                    cACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACttttt                                                                                                                  >  5:262976/1‑134 (MQ=255)
                                     aCCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGGCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTAtgtg                                                                                                    <  5:85117/147‑1 (MQ=255)
                                         cgtcgcTGGTAATGCGAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGTCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGt                                                                                               <  7:172030/145‑1 (MQ=255)
                                              cTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTa                                                                                          <  3:66322/148‑1 (MQ=255)
                                                ggTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTAtt                                                                                        >  7:15344/1‑148 (MQ=255)
                                                  tAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttc                                                                                      >  7:285275/1‑148 (MQ=255)
                                                          gATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTa                                                                                          >  6:227685/1‑136 (MQ=255)
                                                          gATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGGCGTTa                                                                                          <  5:227685/136‑1 (MQ=255)
                                                           aTGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTa                                                                                          >  7:231166/1‑135 (MQ=255)
                                                           aTGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTa                                                                                          <  8:231166/135‑1 (MQ=255)
                                                              tcatcaCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGt                                                                                                                >  6:21813/1‑110 (MQ=255)
                                                              tcatcaCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGt                                                                                                                <  5:21813/110‑1 (MQ=255)
                                                                 tcaCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGa                                                                       <  5:247201/148‑1 (MQ=255)
                                                                  caCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCa                                                                                  <  8:135833/136‑1 (MQ=255)
                                                                  caCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCa                                                                                  >  7:135833/1‑136 (MQ=255)
                                                                   aCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCa                                                                                  >  2:223519/1‑135 (MQ=255)
                                                                   aCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCa                                                                                  <  1:223519/135‑1 (MQ=255)
                                                                   aCGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGaa                                                                      <  4:175430/147‑1 (MQ=255)
                                                                      ggCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttctt                                                                                    <  4:57159/130‑1 (MQ=255)
                                                                      ggCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttctt                                                                                    >  3:57159/1‑130 (MQ=255)
                                                                       gCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGa                                                                 >  3:234070/1‑148 (MQ=255)
                                                                          tGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGATCACAATATGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCg                                                              >  1:323024/1‑148 (MQ=255)
                                                                               cagttgttATAATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATACTCACCATCTGGCTGGCGCATGTCACTGTTTGTACGGCTTATGTCACACTCGTTATTTCTTCAAGATTCCGGCAACTGGAACGCTCGa                                                         >  4:89519/3‑148 (MQ=255)
                                                                               cTGTTGTTATTATTCGTCGCGCTTGCTCATGCCTTTGGCTGGCCTGCGGCCCGGGGTTTACTCACCATCTGGCTGGGGCATGTCACTTTTTGTAGGGGTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGa                                                         <  3:89519/148‑1 (MQ=255)
                                                                               cTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATACTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGa                                                         <  3:215687/148‑1 (MQ=255)
                                                                               cTGGTGTTATTATTAGACGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATACTCACCATCTGGCTGGCGCATTTCACTTTTTGTACGGCTAATGTGGCGGTCGATATTTCTGCAAGATTGCGGGAACTGGATCGCTCg                                                          >  4:215687/1‑147 (MQ=255)
                                                                                     ttattaTTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATag                                                      >  3:77303/1‑145 (MQ=255)
                                                                                        ttattCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGaagaag                                                <  3:292921/148‑1 (MQ=255)
                                                                                         tattCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGaagaag                                                >  2:309149/1‑147 (MQ=255)
                                                                                             cgtcgCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAagcagc                                            <  1:254102/147‑1 (MQ=255)
                                                                                               tcgCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGAt                                         <  8:15344/148‑1 (MQ=255)
                                                                                                  cgcTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGa                                      >  2:144856/1‑148 (MQ=255)
                                                                                                       gCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGAtctc                                  <  1:43205/147‑1 (MQ=255)
                                                                                                          cATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCa                                                                                  >  7:244676/1‑96 (MQ=255)
                                                                                                          cATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCa                                                                                  <  8:244676/96‑1 (MQ=255)
                                                                                                              ccATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTg                                                                            >  6:188467/1‑98 (MQ=255)
                                                                                                              ccATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTg                                                                            <  5:188467/98‑1 (MQ=255)
                                                                                                               cATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCGGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGAcg                         <  5:304807/148‑1 (MQ=255)
                                                                                                                aTTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCg                                 <  8:12588/139‑1 (MQ=255)
                                                                                                                aTTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCg                                 >  7:17561/1‑139 (MQ=255)
                                                                                                                aTTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCg                                 <  8:17561/139‑1 (MQ=255)
                                                                                                                aTTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCg                                 >  7:12588/1‑139 (MQ=255)
                                                                                                                 ttGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGAcgcc                       >  3:162482/1‑148 (MQ=255)
                                                                                                                   ggctggcCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGAcgccg                      <  4:162482/147‑1 (MQ=255)
                                                                                                                   ggctggcCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGAcgccg                      <  4:234070/147‑1 (MQ=255)
                                                                                                                        gcCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGaa                 <  4:77303/147‑1 (MQ=255)
                                                                                                                            gCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGAtt                                                                             <  6:304974/83‑1 (MQ=255)
                                                                                                                            gCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGAtt                                                                             >  5:304974/1‑83 (MQ=255)
                                                                                                                            gCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATagaag                                                   >  2:296819/1‑109 (MQ=255)
                                                                                                                            gCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATagaag                                                   <  1:296819/109‑1 (MQ=255)
                                                                                                                            gCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACAGTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTAAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAgtgt            >  8:317105/1‑148 (MQ=255)
                                                                                                                            gCGGACCGCGGGAGGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAGGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAgtgt            <  5:47923/148‑1 (MQ=255)
                                                                                                                                       tATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCAt  >  2:17029/1‑147 (MQ=255)
                                                                                                                                          gCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGAt                                                        <  6:94633/90‑1 (MQ=255)
                                                                                                                                          gCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGAt                                                        >  5:94633/1‑90 (MQ=255)
                                                                                                                                          gCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATg                                        <  6:299481/106‑1 (MQ=255)
                                                                                                                                          gCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATg                                        >  5:299481/1‑106 (MQ=255)
                                                                                                                                          gCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTc    >  3:201336/1‑142 (MQ=255)
                                                                                                                                          gCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTc    <  4:201336/142‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                               
CAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACGCCGCTGAAAGTGTTTTTCGTCAT  >  NC_000913/897365‑897646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: