| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 909,353 | G→A | 31.7% | A566V (GCG→GTG) | poxB ← | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 909,353 | 0 | G | A | 31.7% | 81.8 / 51.1 | 63 | A566V (GCG→GTG) | poxB | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (21/22); new base A (10/10); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGA > NC_000913/909207‑909500 | aaaCGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGc > 8:69708/1‑148 (MQ=255) aaCGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGc < 1:322766/147‑1 (MQ=255) ccgaaCCTGGGCGGACATCATCGCTTCGAGCATGGAGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCTTGTCATGTCCTTATTATGACGGTATATGCCACCCTTTTTACCTTATCCAGTTTGTTTTCGCCAGTTCGATc < 6:249018/145‑1 (MQ=255) ggcggcCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtcatca < 3:283764/148‑1 (MQ=255) catcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtc < 4:86723/135‑1 (MQ=255) catcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtc > 3:86723/1‑135 (MQ=255) catcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCAc > 1:246274/1‑132 (MQ=255) catcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCAc < 2:246274/132‑1 (MQ=255) gCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCtg < 8:46252/147‑1 (MQ=255) ttCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCCCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgat > 3:56182/1‑147 (MQ=255) ttCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCtgat > 8:149434/1‑147 (MQ=255) cATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcg > 7:270909/1‑148 (MQ=255) cgcgGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgcag < 3:101588/148‑1 (MQ=255) gCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCAAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATTATTGCGCGCAGCata > 5:257164/1‑148 (MQ=255) cTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCatat < 7:69708/148‑1 (MQ=255) tGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCAtata > 3:105612/1‑147 (MQ=255) cGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtcatc < 1:149878/110‑1 (MQ=255) cGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtcatc > 2:149878/1‑110 (MQ=255) cGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGc > 8:105649/1‑148 (MQ=255) cGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAAcc > 8:24366/1‑148 (MQ=255) gTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCtt < 7:24366/148‑1 (MQ=255) aTCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg > 8:318229/1‑148 (MQ=255) cACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACt < 5:84177/92‑1 (MQ=255) cACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACt > 6:84177/1‑92 (MQ=255) cTGCGTAAATCAATCATGGCATGTCCTTATTATGACCGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTAATCACCGAGTCCGCTGATGATTGCGCGCAGCATATACAGTCTGAAAACTTTGACCt > 4:88151/1‑148 (MQ=255) tcaatcaTTGCATGTCCTTATTATGACGTGAAATGCCTCCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGtt < 6:257164/148‑1 (MQ=255) tcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTATTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt > 4:90398/1‑148 (MQ=255) caatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt > 7:331629/1‑148 (MQ=255) atcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGa > 7:192816/1‑148 (MQ=255) atcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGa < 7:105649/148‑1 (MQ=255) tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAgg < 5:9235/119‑1 (MQ=255) tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAgg > 6:9235/1‑119 (MQ=255) gCATGTCCTTATTATGACGGGAACTGCCACCCTTTTTACATTAGCCAGTTTGTTTTAACCAGTTCGATCACTTCATCACCGCTTCAGCTGATGATTGCGCGCAGCATATACAGGATGAAACCTTTGGCCTGTTCGAGTTTGATCTGct > 6:303236/1‑147 (MQ=255) gCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGc < 7:318229/147‑1 (MQ=255) cATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCgg < 1:233104/148‑1 (MQ=255) aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt < 8:331629/148‑1 (MQ=255) gTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtcat > 5:312851/1‑72 (MQ=255) gTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtcat < 6:312851/72‑1 (MQ=255) gTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTg > 1:281008/1‑147 (MQ=255) tCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGa < 7:149434/148‑1 (MQ=255) tCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGa < 4:105612/148‑1 (MQ=255) cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGaa < 8:270909/147‑1 (MQ=255) cTTAATATGACTCGAAATGAAACACTTTTTACCTTAGCCAGTTTGTTTTCGCCATTTCTATCACTTCAGCACCGCGTCATCTGATGATTGAGCGCAGCATATACAGGATGAAACCATTGACCTGTTCGAGTTTGATATGAGGTGTaa < 5:61509/147‑1 (MQ=255) ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGa < 3:91098/145‑1 (MQ=255) ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTACATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGa > 4:91098/1‑145 (MQ=255) tattatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTg > 1:158218/1‑132 (MQ=255) tattatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTg < 2:158218/132‑1 (MQ=255) atGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTa < 2:281008/147‑1 (MQ=255) aCGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAActc < 4:56182/148‑1 (MQ=255) cgaCCCTTTTTACCTTAGCCAGTTTGTTATCACGAGGTCGAGCCCTTCATCCCCGCGTCCGCTGATGATTGCGCGCAGCATATACTGGCTGATACCTTTGGCCTGTTCGTGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTgg < 8:282818/144‑1 (MQ=255) aCCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac > 1:250077/1‑148 (MQ=255) ccTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGa > 2:198160/1‑123 (MQ=255) ccTTTTTACCTTAGCCAGTTTGTGTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGa < 1:198160/123‑1 (MQ=255) gCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacca < 3:90398/147‑1 (MQ=255) aGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAacac > 7:147729/1‑148 (MQ=255) tttgtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCg > 6:109687/1‑148 (MQ=255) ttgtttTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc > 1:192159/1‑128 (MQ=255) ttgtttTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc < 2:192159/128‑1 (MQ=255) ttgtttTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCg < 2:186955/99‑1 (MQ=255) ttgtttTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCg > 1:186955/1‑99 (MQ=255) ttgtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTATGGCGACCACCACATCCACCAACACCg > 1:162998/1‑147 (MQ=255) tgtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTATTCTTTGTCGACCACCACATCCACCAACACCgg < 8:84853/147‑1 (MQ=255) ttCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGt > 5:210373/1‑148 (MQ=255) gCCAGTTCGATCACTTCATCACCGCGGCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGa < 5:97404/148‑1 (MQ=255) | AAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGA > NC_000913/909207‑909500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |