Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,099,421	T→C	10.5%	R181R (CGT→CGC)	ycdX →	alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,099,421	0	T	C	10.5%	104.4 / 4.9	38	R181R (CGT→CGC)	ycdX	alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base T (13/21);  new base C (2/2);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

CCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAAC  >  NC_000913/1099282‑1099565                                                                                                                                            |                                                                                                                                                 ccAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTg                                                                                                                                           <  1:113196/147‑1 (MQ=255) ccAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTg                                                                                                                                           <  3:135046/147‑1 (MQ=255)                aTGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCAtt                                                                                                                           <  6:246199/148‑1 (MQ=255)                 tgtgAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCAtt                                                                                                                           <  2:288175/147‑1 (MQ=255)                 tgtgAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCAtt                                                                                                                           <  6:217240/147‑1 (MQ=255)                         ccGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGa                                                                                                                  >  5:285201/1‑148 (MQ=255)                          cggtGCTGAAGCAGCCGAGAAACATCAGGTGGCGCTGGAAATAAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGAt                                                                                                                 <  6:269813/145‑1 (MQ=255)                               cTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTca                                                                                                            <  4:219519/148‑1 (MQ=255)                                  aaGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCacac                                                                                                         <  6:87725/148‑1 (MQ=255)                                      agcCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACcgcg                                                                                                     >  4:166478/1‑148 (MQ=255)                                          gcgAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCCCGTAAGGGCAGTGAAGACAACTGTCGTGATGTCGCCGCAGCGGTCCGTGAGGCGGGAGGTTGGGGGGCATTAGGCTCGGATTCTCACCCCGCtttgc                                                                                                 >  4:16960/1‑146 (MQ=255)                                            gAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTAcc                                                                                               <  1:316085/148‑1 (MQ=255)                                             aaaCATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCa                                                                                              <  1:261151/148‑1 (MQ=255)                                                      gTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAAt                                                                                     <  5:12787/148‑1 (MQ=255)                                                          cgcTGGAAATCAATAATTCCTCATTTTTACACTCCCGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGa                                                                                 <  3:16960/148‑1 (MQ=255)                                                          cgcTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGa                                                                                 <  6:285201/148‑1 (MQ=255)                                                            cTGGAAATCAATAATTCCTCAGTTTATCACTCCCGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATTCTGGAGGTTGGGTGTCATTAGGCTCGGATTCTAACACCGCGTTTACCATGGGGTAATTTGAag                                                                               <  4:74649/148‑1 (MQ=255)                                                                       taatTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATgg                                                                                           <  3:136573/125‑1 (MQ=255)                                                                       taatTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATgg                                                                                           >  4:136573/1‑125 (MQ=255)                                                                              tCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGCGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAaga                                                                              >  1:153707/1‑131 (MQ=255)                                                                              tCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGCGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAaga                                                                              >  1:158221/1‑131 (MQ=255)                                                                              tCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGCGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAaga                                                                              <  2:153707/131‑1 (MQ=255)                                                                              tCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGCGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAaga                                                                              <  2:158221/131‑1 (MQ=255)                                                                                         cTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGAt                                                  >  2:251695/1‑148 (MQ=255)                                                                                           cACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGttt                                                                                                  >  5:165979/1‑98 (MQ=255)                                                                                           cACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGttt                                                                                                  <  6:165979/98‑1 (MQ=255)                                                                                           cACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGAttt                                                >  4:170133/1‑148 (MQ=255)                                                                                            aCGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGAttt                                                <  3:170133/147‑1 (MQ=255)                                                                                               tAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCg                                            <  3:166478/148‑1 (MQ=255)                                                                                                        tGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTAGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAgcg                                    >  2:61792/1‑147 (MQ=255)                                                                                                         gAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATtctc                                                                                                             <  6:183710/73‑1 (MQ=255)                                                                                                         gAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATtctc                                                                                                             >  5:183710/1‑73 (MQ=255)                                                                                                           aGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCAtt                                >  1:161713/1‑148 (MQ=255)                                                                                                             aCAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCAttt                               >  8:131962/1‑147 (MQ=255)                                                                                                             aCAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGGTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCAttt                               <  7:131962/147‑1 (MQ=255)                                                                                                                aCTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGcc                                          <  2:245433/133‑1 (MQ=255)                                                                                                                aCTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGcc                                          >  1:245433/1‑133 (MQ=255)                                                                                                                                         aCGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAAc  >  7:6875/1‑148 (MQ=255)                                                                                                                                            tGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTa        >  5:289446/1‑139 (MQ=255)                                                                                                                                            tGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTa        <  6:289446/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                 CCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTACGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAAC  >  NC_000913/1099282‑1099565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑