Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,554,103 C→T 12.1% A523T (GCG→ACG)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,1030CT12.1% 160.5 / 11.7 58A523T (GCG→ACG) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base T (2/5);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

GGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAATA  >  NC_000913/1553963‑1554248
                                                                                                                                            |                                                                                                                                                 
ggCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGTAATTgcg                                                                                                                                            <  8:125261/148‑1 (MQ=255)
ggCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTgcg                                                                                                                                            >  2:248387/1‑148 (MQ=255)
  cgcAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGcgcg                                                                                                                                          <  5:132658/148‑1 (MQ=255)
  cgcAGGCGTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGTAATTGcgcg                                                                                                                                          <  4:2506/148‑1 (MQ=255)
     aGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCgg                                                                                                                                         >  5:220641/1‑146 (MQ=255)
       gCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGTAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGgaga                                                                                                                                     <  2:85502/148‑1 (MQ=255)
          tAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGTAATTGCGCGGGAGACtt                                                                                                                                  >  3:222163/1‑148 (MQ=255)
                 aGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGaa                                                                                                                            >  3:42972/1‑147 (MQ=255)
                      cggcggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCTCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCtt                                                                                                                      <  2:225805/148‑1 (MQ=255)
                        gcggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTc                                                                                                                    <  1:248387/148‑1 (MQ=255)
                        gcggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTc                                                                                                                    <  5:209251/148‑1 (MQ=255)
                           gTAGTCGCGGGATTCGGCGTGCCAGAAATTATCGTCAATGGCCTGTTGCACCGCTTCGGCAGAGGTTTTCACCGCCACGAAAAGCTGCTGCGCCATTTTGCCAACCCCAAACGCAATTGCGCGGCAGAATTTCTGAATATCTTACAGt                                                                                                                 >  1:117417/1‑148 (MQ=255)
                              gTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCGATTTTGCCATCGGCAAACGTAATTGCGCGGGAGACTTTCTGAATATCTTTGAGTTc                                                                                                               <  4:113295/147‑1 (MQ=255)
                                 gcgGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGc                                                                                                           <  5:156054/148‑1 (MQ=255)
                                        tCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGc                                                                                                           >  4:238194/1‑141 (MQ=255)
                                        tCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGc                                                                                                           <  3:238194/141‑1 (MQ=255)
                                               gCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCGTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCt                                                                                               <  6:220641/146‑1 (MQ=255)
                                                  aGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGcc                                                                                          >  6:105331/1‑148 (MQ=255)
                                                    aaaTTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGt                                                                                        >  4:89664/1‑148 (MQ=255)
                                                     aaTTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGcgcg                                                                                                                                          <  1:97487/97‑1 (MQ=255)
                                                     aaTTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGcgcg                                                                                                                                          >  2:97487/1‑97 (MQ=255)
                                                      aTTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTc                                                                                      <  2:318672/148‑1 (MQ=255)
                                                               aaTGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCaa                                                                              <  3:106645/147‑1 (MQ=255)
                                                                        ttGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAAt                                                                      <  2:326909/146‑1 (MQ=255)
                                                                          gCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGTAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACt                                                                   <  4:222163/147‑1 (MQ=255)
                                                                           cAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTgc                                                                 >  3:128546/1‑148 (MQ=255)
                                                                           cAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTgc                                                                 >  6:24919/1‑148 (MQ=255)
                                                                             gggCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTgcgc                                                               >  8:234564/1‑148 (MQ=255)
                                                                                   cGGCCGAGGTTATGACCGCCACGCCTTGCTGCTGCGCCTTTTTTCGAACCGCAAACTCATTGTCGCGGGAGCCGTTCTGAATATCTTTCCGGTCCGGCAGTACCTTACGTTCGCCGTTCAGCACCCAAGGGGAATACTGCGCCAgtg                                                          <  2:117417/147‑3 (MQ=255)
                                                                                   cGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCg                                                          <  3:89664/147‑1 (MQ=255)
                                                                                     gCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGttt                                                       <  4:42972/148‑1 (MQ=255)
                                                                                        gagGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCAc                                                    <  7:234564/148‑1 (MQ=255)
                                                                                         agGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAACCGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATg                                                                            >  5:111364/1‑123 (MQ=255)
                                                                                         agGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATg                                                                            <  6:111364/123‑1 (MQ=255)
                                                                                            ttttCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGc                                                <  5:105331/148‑1 (MQ=255)
                                                                                             tttCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGcc                                               >  7:127174/1‑148 (MQ=255)
                                                                                             tttCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGcc                                               >  7:257809/1‑148 (MQ=255)
                                                                                                 aCCGCCACGCCTTGCTGCTGCGCCA‑TTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACAt                                          >  2:159111/1‑148 (MQ=255)
                                                                                                  ccGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGtt                                                        >  3:92410/1‑134 (MQ=255)
                                                                                                  ccGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGtt                                                        <  4:92410/134‑1 (MQ=255)
                                                                                                      cACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGtt                                                                                                                <  3:21855/74‑1 (MQ=255)
                                                                                                      cACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGtt                                                                                                                >  4:21855/1‑74 (MQ=255)
                                                                                                       aCGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCa                                     >  5:162505/1‑148 (MQ=255)
                                                                                                       aCGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGATCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCa                                     >  8:204340/1‑148 (MQ=255)
                                                                                                                  ctgcGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGt                           <  7:204340/147‑1 (MQ=255)
                                                                                                                  ctgcGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTg                          <  5:24919/148‑1 (MQ=255)
                                                                                                                  ctgcGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTg                          <  4:128546/148‑1 (MQ=255)
                                                                                                                       ccATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATgg                                                                           >  5:244759/1‑94 (MQ=255)
                                                                                                                       ccATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATgg                                                                           <  6:244759/94‑1 (MQ=255)
                                                                                                                       ccATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACg                     <  8:257809/148‑1 (MQ=255)
                                                                                                                           tttGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAc                 >  2:253672/1‑148 (MQ=255)
                                                                                                                           tttGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAc                 >  8:244994/1‑148 (MQ=255)
                                                                                                                             tGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAcg                <  7:271100/147‑1 (MQ=255)
                                                                                                                                 aaCCGCAAACGTAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATa                                                                                                  >  6:18503/1‑61 (MQ=255)
                                                                                                                                 aaCCGCAAACGTAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATa                                                                                                  <  5:18503/61‑1 (MQ=255)
                                                                                                                                      cAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAg       >  2:191695/1‑147 (MQ=255)
                                                                                                                                          cGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAATa  <  7:244994/148‑1 (MQ=255)
                                                                                                                                          cGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAATa  <  1:253672/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
GGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAATA  >  NC_000913/1553963‑1554248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑