Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,135,369 C→T 5.1% R95H (CGT→CAT)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,135,3690CT 5.1% 257.6 / 3.0 78R95H (CGT→CAT) wzccolanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (36/38);  new base T (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GCGGTTAAAGGTGGTCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAGGTGTAAACC  >  NC_000913/2135224‑2135515
                                                                                                                                                  |                                                                                                                                                  
gCGGTTAAAGGTGGTCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGcgaacga                                                                                                                                                  <  8:62883/148‑1 (MQ=255)
 cGGTTTAAGGTGGTCTGTTTGTCCGTCTGGTTCTGTCGTCCCTTCAGGCGATCCGAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGcgaacgaa                                                                                                                                                 <  6:80851/148‑1 (MQ=255)
        aGGTGGTCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAACACCAGACGcgaacgaa                                                                                                                                                 <  5:2444/141‑1 (MQ=255)
        aGGTGGTCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAACACCAGACGcgaacgaa                                                                                                                                                 >  6:2444/1‑141 (MQ=255)
              tCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAAc                                                                                                                                            <  7:187283/140‑1 (MQ=255)
              tCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAAc                                                                                                                                            >  8:187283/1‑140 (MQ=255)
                   ttCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGAtctc                                                                                                                                    >  5:235576/1‑143 (MQ=255)
                   ttCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGAtctc                                                                                                                                    <  6:235576/143‑1 (MQ=255)
                   ttCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGt                                                                                                                               >  7:102521/1‑148 (MQ=255)
                    tCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTc                                                                                                                              >  8:209161/1‑148 (MQ=255)
                       ccGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGa                                                                                                                           >  3:91349/1‑148 (MQ=255)
                             cGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAAc                                                                                                                                            <  3:281580/125‑1 (MQ=255)
                             cGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAAc                                                                                                                                            >  4:281580/1‑125 (MQ=255)
                               ttCTGACGTCCCATCAGGCGTTCGCAGCACGCTCCG‑AAAATCGGGAACGTTTTTTTGCTCACTGGAATATCGAGGTCGAGTTCGTCGGGCGTTTTACCAAGCACCAGACGGGTACGAATCAACTGGATCTCGGCGTCCGATGCAGGCg                                                                                                                   >  5:87838/1‑148 (MQ=255)
                                tCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCa                                                                                                                                              <  8:253595/120‑1 (MQ=255)
                                tCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCa                                                                                                                                              >  7:253595/1‑120 (MQ=255)
                                    aCGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACt                                                                                                                                           >  1:49522/1‑119 (MQ=255)
                                    aCGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACt                                                                                                                                           <  2:49522/119‑1 (MQ=255)
                                    aCGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGAt                                                                                                                          <  8:167725/136‑1 (MQ=255)
                                    aCGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGAt                                                                                                                          >  7:167725/1‑136 (MQ=255)
                                    aCGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGtt                                                                                                                <  4:26241/146‑1 (MQ=255)
                                      gTCCCATCAGGCGATCCCAGCCCGCACTG‑AAAATCGGGAACGTGTTTTGTCTCACTGCAATTTCGAGTTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACTAATCAACTGTATCTCGGCGTCCGATGCAGGCGGTttgt                                                                                                             <  4:237399/147‑1 (MQ=255)
                                      gTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgtt                                                                                                            <  6:126912/148‑1 (MQ=255)
                                       tCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgttg                                                                                                           <  8:102521/148‑1 (MQ=255)
                                       tCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCAATGCAATATCGAGGTCGAGATCTTCCACCGTTTTACCAATCACCAGACGCGTACGAATCAACTGGATCTCGTCGTCCGATGCAGGCGGTgtgttg                                                                                                           <  6:87838/148‑1 (MQ=255)
                                         ccATCAGGCGATCCCAGCCCGCACCGAAAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgttg                                                                                                           <  7:315794/147‑1 (MQ=255)
                                         ccATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTgg                                                                                                                                         <  7:912/116‑1 (MQ=255)
                                         ccATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTgg                                                                                                                                         >  8:912/1‑116 (MQ=255)
                                          cATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCt                                                                                                        <  3:122198/148‑1 (MQ=255)
                                           aTCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCt                                                                                                        >  2:4685/1‑147 (MQ=255)
                                                  gATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGa                                                                                                <  4:109963/148‑1 (MQ=255)
                                                   aTCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGaa                                                                                               <  7:1381/148‑1 (MQ=255)
                                                    tCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgttg                                                                                                           >  3:10929/1‑135 (MQ=255)
                                                    tCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgttg                                                                                                           <  4:10929/135‑1 (MQ=255)
                                                    tCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCAGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAAc                                                                                              >  1:255174/1‑148 (MQ=255)
                                                     cccAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAAc                                                                                              >  8:151353/1‑147 (MQ=255)
                                                      ccAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCa                                                                                            <  4:91349/148‑1 (MQ=255)
                                                       cAGCCCTCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCg                                                                                                                            >  4:178650/1‑115 (MQ=255)
                                                       cAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCg                                                                                                                            <  3:178650/115‑1 (MQ=255)
                                                       cAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCaa                                                                                           <  1:141356/148‑1 (MQ=255)
                                                        aGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAATGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAAt                                                                                          >  8:313519/1‑148 (MQ=255)
                                                        aGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACAAGACGCGAATGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAAt                                                                                          >  8:309035/1‑148 (MQ=255)
                                                           ccGCACCG‑AAAATCGGGAACGGGTGTGTGCTCACTGCAATATCGAGGTCGAGATCGGCCACCGATTTACCAAGCACCAGACGCGAACGAATCAACTGGATCGCGGCGGACGATGCAGGCGGTTTGTTGGGTAATGCCGAACCAATggc                                                                                       >  5:226924/1‑146 (MQ=255)
                                                                ccG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgt                                                                                                             >  4:260844/1‑121 (MQ=255)
                                                                ccG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTttgt                                                                                                             <  3:260844/121‑1 (MQ=255)
                                                                ccG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATg                                                                                                    <  5:193738/130‑1 (MQ=255)
                                                                ccG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATg                                                                                                    >  6:193738/1‑130 (MQ=255)
                                                                 cG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGc                                                                                                                        <  1:247542/109‑1 (MQ=255)
                                                                 cG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGc                                                                                                                        >  2:247542/1‑109 (MQ=255)
                                                                  g‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTa                                                                                                       >  1:323822/1‑125 (MQ=255)
                                                                  g‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTa                                                                                                       <  2:323822/125‑1 (MQ=255)
                                                                  g‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCAc                                                                                 >  4:147652/1‑147 (MQ=255)
                                                                     aaaTCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTaa                                                                              <  1:4685/148‑1 (MQ=255)
                                                                            gAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTg                                                                       <  3:147652/148‑1 (MQ=255)
                                                                                    ttttGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCaa                                                                                           <  6:31997/120‑1 (MQ=255)
                                                                                    ttttGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCaa                                                                                           >  5:31997/1‑120 (MQ=255)
                                                                                    ttttGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGtt                                                               >  5:20889/1‑148 (MQ=255)
                                                                                        gCTCACTGCAATATCGAGGTCGAGAGCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTg                                                            <  2:58902/147‑1 (MQ=255)
                                                                                          tCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGTATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAAtt                                                                        <  5:217519/133‑1 (MQ=255)
                                                                                           cACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGtttt                                                             <  3:18814/143‑1 (MQ=255)
                                                                                           cACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGtttt                                                             >  4:18814/1‑143 (MQ=255)
                                                                                           cACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTc                                                         >  6:176096/1‑147 (MQ=255)
                                                                                            aCTGCAATATCGAGGTCGAGATCGTCCTCCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTACTGCACTAACTAATTGCCGCTGTTTTGCTCGa                                                       <  8:186436/148‑1 (MQ=255)
                                                                                             cTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGAt                                                      <  7:151353/148‑1 (MQ=255)
                                                                                             cTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGAt                                                      >  8:21436/1‑148 (MQ=255)
                                                                                              tGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGAtt                                                     >  2:315042/1‑148 (MQ=255)
                                                                                               gCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGAttt                                                    >  1:37967/1‑148 (MQ=255)
                                                                                                 aaTATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTg                                                   >  5:25019/1‑147 (MQ=255)
                                                                                                  atatCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTa                                                 <  6:20889/148‑1 (MQ=255)
                                                                                                    atCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAgg                                                                                                                     >  5:41857/1‑78 (MQ=255)
                                                                                                    atCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAgg                                                                                                                     <  6:41857/78‑1 (MQ=255)
                                                                                                          gTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCg                                         <  7:209161/148‑1 (MQ=255)
                                                                                                             gagaTCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCg                                      <  5:176096/148‑1 (MQ=255)
                                                                                                               gaTCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGc                                    <  7:21436/148‑1 (MQ=255)
                                                                                                                 tCGTCCACCGTTTTACCAAGCACCAGACGCGAATGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCAc                                  <  7:313519/148‑1 (MQ=255)
                                                                                                                 tCGTCCACCGTTTTACCAAGCACCAGACGCGAATGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCAc                                  <  7:309035/148‑1 (MQ=255)
                                                                                                                    tCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGaa                                                                          <  6:300713/105‑1 (MQ=255)
                                                                                                                    tCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGaa                                                                          >  5:300713/1‑105 (MQ=255)
                                                                                                                         cGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATc                          >  4:156687/1‑148 (MQ=255)
                                                                                                                                 cAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGc                  >  7:327027/1‑148 (MQ=255)
                                                                                                                                         aGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAgg          >  6:66332/1‑148 (MQ=255)
                                                                                                                                         aGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAgg          >  8:227663/1‑148 (MQ=255)
                                                                                                                                              cgaacgaaTCAACTGGATCTAGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAGGTGTaa     <  5:66332/148‑1 (MQ=255)
                                                                                                                                                aacgaaTCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAGGTGTAAAc   >  6:183588/1‑148 (MQ=255)
                                                                                                                                                 acgaaTCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAGGTGTAAAcc  <  1:315042/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCGGTTAAAGGTGGTCACTTTCACCGTCTCGTTCTGACGTCCCATCAGGCGATCCCAGCCCGCACCG‑AAAATCGGGAACGTGTTTTTGCTCACTGCAATATCGAGGTCGAGATCGTCCACCGTTTTACCAAGCACCAGACGCGAACGAATCAACTGGATCTCGGCGTCCGATGCAGGCGGTTTGTTGGCTAATGCCGAACCAATGTCCTGCACTAACGAATTGCCGCTGTTTTGCTCGATTTGTACCAGTGCGTCGGCACTATAAATCGGCGTGGCGAAGAAGGTGTAAACC  >  NC_000913/2135224‑2135515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑