Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,687,166 C→T 5.3% A81A (GCG→GCA glnB ← regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,687,1660CT 5.3% 248.6 / 2.8 75A81A (GCG→GCAglnBregulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (31/40);  new base T (2/2);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

CTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTT  >  NC_000913/2687020‑2687309
                                                                                                                                                  |                                                                                                                                               
cTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGAGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTgcg                                                                                                                                                >  2:303109/1‑148 (MQ=255)
       ttGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGACCTCTTCACCGGTACGGATGTGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGATTGCGCCGTGc                                                                                                                                          >  8:80737/1‑147 (MQ=255)
           tGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaata                                                                                                                                     <  8:304881/148‑1 (MQ=255)
             ttGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaataat                                                                                                                                   <  1:77728/148‑1 (MQ=255)
             ttGCCAGAGGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaataat                                                                                                                                   <  5:86011/148‑1 (MQ=255)
               gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCTAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCTTGCGAATAATg                                                                                                                                  <  1:174268/147‑1 (MQ=255)
                ccGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCCCCGATTTTGCCGGTTTGCGCCGTGCGAATAATgg                                                                                                                                 >  1:203088/1‑147 (MQ=255)
                ccGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATgg                                                                                                                                 >  6:303698/1‑147 (MQ=255)
                ccGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGt                                                                                                                                <  5:8414/148‑1 (MQ=255)
                 cGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaata                                                                                                                                     >  5:175221/1‑142 (MQ=255)
                 cGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaata                                                                                                                                     <  6:175221/142‑1 (MQ=255)
                 cGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTa                                                                                                                               <  2:191128/148‑1 (MQ=255)
                    aTGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTATCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg                                                                                                                            <  8:22148/148‑1 (MQ=255)
                    aTGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTATCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg                                                                                                                            <  8:27291/148‑1 (MQ=255)
                    aTGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGTGCCGTGCGAATAATGGTATc                                                                                                                             >  5:105002/1‑147 (MQ=255)
                     tGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGTGCCGTGCGAATAATGGTATCGa                                                                                                                           <  6:105002/148‑1 (MQ=255)
                      ggAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGa                                                                                                                           >  7:216155/1‑147 (MQ=255)
                       gAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt                                                                                                                              >  7:242439/1‑143 (MQ=255)
                       gAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt                                                                                                                              <  8:242439/143‑1 (MQ=255)
                       gAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGac                                                                                                                          <  5:96496/147‑1 (MQ=255)
                          aCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTa                                                                                                                               >  5:23517/1‑139 (MQ=255)
                          aCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTa                                                                                                                               <  6:23517/139‑1 (MQ=255)
                            aTCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAgg                                                                                                                     >  7:109194/1‑147 (MQ=255)
                             tCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg                                                                                                                            >  7:325368/1‑139 (MQ=255)
                             tCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg                                                                                                                            <  8:325368/139‑1 (MQ=255)
                              ccGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTAt                                                                                                                  >  8:176475/1‑148 (MQ=255)
                                   aaCCCTTGACGCGGTTTAAATTGGCGCGTCGTCCTCTTGACCGGTACGGATGTGAATGACCCGTGCCACGTCATAGACGAAGATTTTACCGTCAGCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACa                                                                                                             <  7:80737/148‑1 (MQ=255)
                                   aaCCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACa                                                                                                             <  2:295897/148‑1 (MQ=255)
                                   aaCCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACa                                                                                                             <  8:216155/148‑1 (MQ=255)
                                         tGACGCGGTTTAAATTGCCGGGTCGTGCTCTTCACCGGTACGGATGCGAATGACGCGTGCGACGTCATTGACGAAGATTTTACCGTCACCGATTTTTCCGGTTTGCGACGTGCGAATAATGGTTTCGActca                                                                                                                       <  3:280380/132‑1 (MQ=255)
                                            cgcgGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgtc                                                                                                        >  4:160552/1‑144 (MQ=255)
                                            cgcgGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgtc                                                                                                        <  3:160552/144‑1 (MQ=255)
                                                  ttAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACaa                                                                                                            <  3:242548/134‑1 (MQ=255)
                                                  ttAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACaa                                                                                                            >  4:242548/1‑134 (MQ=255)
                                                   tAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGac                                                                                                                          >  4:159543/1‑119 (MQ=255)
                                                   tAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGac                                                                                                                          <  3:159543/119‑1 (MQ=255)
                                                       ttGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGGGCCACGTCATAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAt                                                                                         <  5:52692/148‑1 (MQ=255)
                                                        tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtc                                                                                        >  8:206659/1‑148 (MQ=255)
                                                        tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGAGTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtc                                                                                        >  5:297937/1‑148 (MQ=255)
                                                                   ccTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTc                                                                               >  8:193201/1‑146 (MQ=255)
                                                                   ccTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTc                                                                               <  7:193201/146‑1 (MQ=255)
                                                                   ccTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCAc                                                                             >  4:198784/1‑148 (MQ=255)
                                                                      ctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCa                                                                                     >  5:26670/1‑137 (MQ=255)
                                                                      ctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCa                                                                                     <  6:26670/137‑1 (MQ=255)
                                                                      ctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACttt                                                                          >  3:34112/1‑148 (MQ=255)
                                                                      ctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACttt                                                                          >  1:94439/1‑148 (MQ=255)
                                                                         cACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACttt                                                                          <  8:109194/145‑1 (MQ=255)
                                                                               tACTGATTCGATTGACCCGTGCCACGTCAATTACTAAGATTTTACCGTCACGGATTTTGACGGTTTGCGCCGTGCGAATAATGTTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGaaa                                                                 <  2:94439/148‑1 (MQ=255)
                                                                               tACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGaaa                                                                 <  6:22144/148‑1 (MQ=255)
                                                                                      gCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATccac                                                           >  7:53636/1‑147 (MQ=255)
                                                                                       cGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATccacc                                                          <  4:34112/147‑1 (MQ=255)
                                                                                        gAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCat                                                        <  7:176475/148‑1 (MQ=255)
                                                                                         aaTGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGAGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCata                                                       >  8:93543/1‑148 (MQ=255)
                                                                                          aTGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCatat                                                      <  4:123684/148‑1 (MQ=255)
                                                                                          aTGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCatat                                                      <  7:206659/148‑1 (MQ=255)
                                                                                          aTGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCata                                                       >  2:290336/1‑147 (MQ=255)
                                                                                           tGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCAtata                                                     <  5:303698/148‑1 (MQ=255)
                                                                                            gACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgtcgt                                                                                                      >  4:133485/1‑98 (MQ=255)
                                                                                            gACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATgtcgt                                                                                                      <  3:133485/98‑1 (MQ=255)
                                                                                             aCCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACt                                                   <  8:53636/148‑1 (MQ=255)
                                                                                                   gCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtct                                                                                       >  6:56127/1‑106 (MQ=255)
                                                                                                   gCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtct                                                                                       <  5:56127/106‑1 (MQ=255)
                                                                                                        gTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCgg                                        <  2:32329/148‑1 (MQ=255)
                                                                                                          cAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCat                                                        >  6:102180/1‑130 (MQ=255)
                                                                                                          cAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCat                                                        <  5:102180/130‑1 (MQ=255)
                                                                                                                  aaGATTTTACCGTCACCGATTTTGCCGGTTTGTGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCgg                              >  1:260110/1‑148 (MQ=255)
                                                                                                                  aaGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCAGCGCCGCGGTACAGCTCgg                              >  3:81964/1‑148 (MQ=255)
                                                                                                                   aGATTTTACCGTCACCGATTTTGCCGGTTTGTGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGt                             <  2:260110/148‑1 (MQ=255)
                                                                                                                   aGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGt                             <  7:93543/148‑1 (MQ=255)
                                                                                                                             gTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCACCGCGGTACAGCTCGGTATGGCCTTTc                   >  1:293412/1‑148 (MQ=255)
                                                                                                                                  cGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGc               >  8:219819/1‑147 (MQ=255)
                                                                                                                                      tttGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGAcc           >  5:164428/1‑147 (MQ=255)
                                                                                                                                       ttGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACGATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCa          <  6:297937/147‑1 (MQ=255)
                                                                                                                                            ggTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCt    >  2:312763/1‑148 (MQ=255)
                                                                                                                                             gTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCtt   <  1:290336/148‑1 (MQ=255)
                                                                                                                                              tttGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCttt  <  1:312763/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTT  >  NC_000913/2687020‑2687309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: