Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,534,738 T→C 5.5% G377G (GGA→GGG envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,534,7380TC 5.5% 224.3 / 2.6 72G377G (GGA→GGGenvZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base C (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCA  >  NC_000913/3534594‑3534885
                                                                                                                                                |                                                                                                                                                   
gctGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTcc                                                                                                                                                   <  7:143628/147‑1 (MQ=255)
   ggTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGAc                                                                                                                                              >  5:228720/1‑149 (MQ=255)
       ccAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGt                                                                                                                                           <  8:19195/148‑1 (MQ=255)
        cAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGACGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCa                                                                                                                                         >  5:221016/1‑149 (MQ=255)
               cAGCATCCCGTTATGGTTATCCACGATACGCTTCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCa                                                                                                                                  <  1:114996/149‑1 (MQ=255)
               cAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTc                                                                                                                                    <  8:50170/147‑1 (MQ=255)
               cAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTc                                                                                                                                    <  3:333459/147‑1 (MQ=255)
               cAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCa                                                                                                                                  <  5:69647/149‑1 (MQ=255)
               cAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATCCCCGGACCGTCCTCTTCCa                                                                                                                                  >  1:50261/1‑149 (MQ=255)
               cAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATCCCCGGACCGTCATCTTCCa                                                                                                                                  >  1:44881/1‑149 (MQ=255)
                   aTCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTg                                                                                                                              >  7:29113/1‑149 (MQ=255)
                    tCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTg                                                                                                                              >  5:12021/1‑148 (MQ=255)
                      ccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGaa                                                                                                                           >  5:23240/1‑149 (MQ=255)
                        gTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAAc                                                                                                                          >  5:342223/1‑148 (MQ=255)
                               ttATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGAcc                                                                                                                                             <  8:353661/122‑1 (MQ=255)
                               ttATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGAGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGAcc                                                                                                                                             >  7:353661/1‑122 (MQ=255)
                                 aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGa                                                                                                                 <  6:12021/148‑1 (MQ=255)
                                  tCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTc                                                                                                                                          <  4:189000/122‑1 (MQ=255)
                                     aCGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCgg                                                                                                            <  2:55233/149‑1 (MQ=255)
                                         tACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTcc                                                                                                        <  8:29113/149‑1 (MQ=255)
                                             cTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGAGAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTc                                                                                                    >  7:10837/1‑149 (MQ=255)
                                             cTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCg                                                                                                                                            <  5:63444/109‑1 (MQ=255)
                                             cTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCg                                                                                                                                            >  6:63444/1‑109 (MQ=255)
                                                   aaTTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCgctgc                                                                                              >  2:14413/1‑149 (MQ=255)
                                                   aaTTGCCAGCCCGAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTCGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCCTCATCTTCCACCTGGACCCAGGCGCGATTCGGCTCCGTTCCgctgc                                                                                              >  3:254529/1‑149 (MQ=255)
                                                     ttGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCgctgct                                                                                             >  3:87611/1‑148 (MQ=255)
                                                        ccAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACt                                                                                         >  1:190166/1‑149 (MQ=255)
                                                               tAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATc                                                                                   >  6:173479/1‑148 (MQ=255)
                                                                 aTCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAg                                                                                >  6:91650/1‑149 (MQ=255)
                                                                 aTCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAg                                                                                <  4:165737/149‑1 (MQ=255)
                                                                    ccGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCa                                                                             >  6:238584/1‑149 (MQ=255)
                                                                    ccGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCa                                                                             >  4:54189/1‑149 (MQ=255)
                                                                    ccGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCa                                                                             <  6:342223/149‑1 (MQ=255)
                                                                      gTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCgct                                                                                                >  6:244901/1‑128 (MQ=255)
                                                                      gTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCgct                                                                                                <  5:244901/128‑1 (MQ=255)
                                                                      gTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATCCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCAtt                                                                           <  2:50261/149‑1 (MQ=255)
                                                                      gTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATCCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCAtt                                                                           <  2:44881/149‑1 (MQ=255)
                                                                          cGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCa                                                                       >  4:295437/1‑149 (MQ=255)
                                                                                tGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTg                                                                                      >  1:352731/1‑128 (MQ=255)
                                                                                tGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTg                                                                                      <  2:352731/128‑1 (MQ=255)
                                                                                  gTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGggc                                                               <  6:228720/149‑1 (MQ=255)
                                                                                      gcgcACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGATCCGCTGCTGACTTTGATCCAGCCATTGCCATAAAGGgcggcg                                                           >  2:73560/1‑149 (MQ=255)
                                                                                       cgcACTGTCGCCGCGGACAAACGGCTGGACCAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCAACTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGt                                                          >  4:32456/1‑149 (MQ=255)
                                                                                            tGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGac                                                      >  2:355562/1‑148 (MQ=255)
                                                                                                         aaaCGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCa                                        >  6:236685/1‑149 (MQ=255)
                                                                                                               cTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACcgcgc                                  >  6:198121/1‑149 (MQ=255)
                                                                                                                  gAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGtt                               <  2:190166/149‑1 (MQ=255)
                                                                                                                  gAACAGGTGCTTACGTTGGTCCGGCGCAATTCCCGGACCGGCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGtt                               <  7:333636/149‑1 (MQ=255)
                                                                                                                   aaCAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGttt                              >  5:104392/1‑149 (MQ=255)
                                                                                                                    aCAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGttt                              <  6:104392/148‑1 (MQ=255)
                                                                                                                       ggTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATc                          >  2:325648/1‑149 (MQ=255)
                                                                                                                        gTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGa                                                                                     >  3:127068/1‑89 (MQ=255)
                                                                                                                        gTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGa                                                                                     <  4:127068/89‑1 (MQ=255)
                                                                                                                        gTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATc                          >  4:287846/1‑148 (MQ=255)
                                                                                                                        gTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCg                         >  5:213833/1‑149 (MQ=255)
                                                                                                                           cTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCGTTGCCATAACGGGCGGCGTTGACCACAATATTCGCCACCGCGCGTTTGATCGa                        >  5:344243/1‑147 (MQ=255)
                                                                                                                           cTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACcgc                                    <  4:70866/135‑1 (MQ=255)
                                                                                                                           cTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACcgc                                    >  3:70866/1‑135 (MQ=255)
                                                                                                                           cTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGa                        >  1:38401/1‑147 (MQ=255)
                                                                                                                            ttACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGtt                               <  2:373452/139‑1 (MQ=255)
                                                                                                                            ttACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGtt                               >  1:373452/1‑139 (MQ=255)
                                                                                                                              aCGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGaccacc                                                  <  4:1108/118‑1 (MQ=255)
                                                                                                                               cGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCgg                  <  1:355562/149‑1 (MQ=255)
                                                                                                                                    ttCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCa             <  6:221016/149‑1 (MQ=255)
                                                                                                                                     tCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCAt            >  3:230520/1‑149 (MQ=255)
                                                                                                                                      ccGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCAtt           >  4:140498/1‑149 (MQ=255)
                                                                                                                                        ggCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCAtttt         <  5:91650/149‑1 (MQ=255)
                                                                                                                                        ggCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCAtttt         <  3:295437/149‑1 (MQ=255)
                                                                                                                                           gcAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCAc      <  6:23240/149‑1 (MQ=255)
                                                                                                                                            cAATTCCCGGACCGTCATGTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTTCTGACTTTGATCCAGCCATTGCGATATCGGGCGGCGTTGACCACCATATTCGCCTCCGCGCGTTTGATCGACAGCGGGTGCATTTTCACt     <  4:254529/149‑1 (MQ=255)
                                                                                                                                             aaTTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACtt    <  4:87611/149‑1 (MQ=255)
                                                                                                                                              aTTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTc   <  1:14413/149‑1 (MQ=255)
                                                                                                                                               ttCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGGATTTTCACTTCa  <  6:69270/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTGCTGACTTTGATCCAGCCATTGCCATAACGGGCGGCGTTGACCACCATATTCGCCACCGCGCGTTTGATCGACAGCGGGTGCATTTTCACTTCA  >  NC_000913/3534594‑3534885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: