Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 556,492 | T→C | 7.1% | K21R (AAA→AGA) | ybcI ← | DUF457 family inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 556,492 | 0 | T | C | 7.1% | 165.3 / 3.0 | 56 | K21R (AAA→AGA) | ybcI | DUF457 family inner membrane protein |
Reads supporting (aligned to +/- strand): ref base T (29/23); new base C (2/2); total (31/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 |
CAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGT > NC_000913/556349‑556639 | caaACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAAAAACAGACGCGGAGGGATGACGTTctca > 3:10308/1‑146 (MQ=255) aCGCAAACAACAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaaccc < 1:300731/149‑1 (MQ=255) taGCGAATGGGTAAACCCGCGATTACAAAATTCATTGCCGTAAGCAACGCCAAATTTTAACGACAATACGTGGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAAc < 4:10308/148‑1 (MQ=255) aGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAAc > 3:210472/1‑148 (MQ=255) ggTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTa > 7:300769/1‑137 (MQ=255) ggTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTa < 8:300769/137‑1 (MQ=255) gTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGGTGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAg > 8:141335/1‑149 (MQ=255) aaaCCCGCGATGACCCAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGAGGACTTTCGAACCCAACCCTAAACCAATGCATAggg > 7:160996/1‑149 (MQ=255) aaCCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAgggg < 1:73998/149‑1 (MQ=255) aCCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAgggg > 4:175855/1‑148 (MQ=255) ccGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAACCCAATGCATAGGGGAAc > 4:195843/1‑149 (MQ=255) ccGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAAc > 4:100955/1‑149 (MQ=255) ccAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtg > 1:101440/1‑149 (MQ=255) cAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACACAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgc > 2:228058/1‑149 (MQ=255) aaaaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgc > 4:233080/1‑148 (MQ=255) aaaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCa < 5:278125/128‑1 (MQ=255) aaaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCa > 6:278125/1‑128 (MQ=255) aaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATCCGTCGGCGTCGGGCAGCATCGCCAGGATGATGCCGGCAACTAACAGACGCGGAGGGATGACGTTCGAACCCAACCCTAAACCAAGGCGGGGGGGACCGGGGgcgggcgt > 4:228576/1‑148 (MQ=255) cATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGtaata < 8:28229/149‑1 (MQ=255) aTTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGtaataa > 5:282680/1‑149 (MQ=255) gCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACg < 6:243608/148‑1 (MQ=255) gTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTg < 8:160996/149‑1 (MQ=255) gCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACttt > 5:224330/1‑89 (MQ=255) gCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACttt < 6:224330/89‑1 (MQ=255) gCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCat < 3:175855/149‑1 (MQ=255) aaCGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCatga > 3:320397/1‑149 (MQ=255) aCGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCatgat < 3:270761/149‑1 (MQ=255) gCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGCTGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATgg < 7:141335/149‑1 (MQ=255) aaaCGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCgg > 4:235144/1‑149 (MQ=255) gtcggcgtcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGAt < 3:100955/149‑1 (MQ=255) gtcggcgtcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTCTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGAt > 4:245970/1‑149 (MQ=255) gtcggcgtcggGCAGCATCGCCAGGATGAGTCCGGCAAATAACAGACGCGGAGGGATGCCTCTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGTTCGCTTCTGCAAAATGTAGAt > 4:241373/1‑149 (MQ=255) tcggcgtcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATg < 3:235144/149‑1 (MQ=255) tcggcggcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATg < 4:210472/149‑1 (MQ=255) cggcgtcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGc > 2:269758/1‑149 (MQ=255) tcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCa < 3:195843/149‑1 (MQ=255) aTCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTa > 4:312698/1‑137 (MQ=255) aTCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTa < 3:312698/137‑1 (MQ=255) tCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGc < 6:269337/115‑1 (MQ=255) tCGCAAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGc > 5:269337/1‑115 (MQ=255) ggATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACgggg > 5:329981/1‑149 (MQ=255) atTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGa < 7:358153/135‑1 (MQ=255) atTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGa > 8:358153/1‑135 (MQ=255) ccGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTagag < 2:101440/148‑1 (MQ=255) cGGCAAATAACTGACGAGGAGGGATGAATTTCGAACCCAACGCTAAACCATTGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACggg < 4:187771/139‑1 (MQ=255) cGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAggg > 6:249883/1‑149 (MQ=255) cGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACCGCGGCGTGCGTAATAACGGATGGCAAGATGGTCGCTTCCGCAAAATGTCGAAGCTATCAGCATGGATGAACggg > 3:187771/1‑139 (MQ=255) tAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGt > 1:122601/1‑149 (MQ=255) aaCAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTc > 4:53085/1‑149 (MQ=255) gcgGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGaa > 4:96015/1‑146 (MQ=255) gcgGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGaa > 4:91325/1‑146 (MQ=255) cgGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAaga < 6:329981/149‑1 (MQ=255) aTGACTTTCGAACCCAACCCTAACCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAATTATGAAAAGAGAACCgg > 4:210193/1‑149 (MQ=255) tGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGaa > 5:218375/1‑144 (MQ=255) tGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGaa < 6:218375/144‑1 (MQ=255) tGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGAGGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGc < 3:233080/149‑1 (MQ=255) tGACTCTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGc < 3:245970/149‑1 (MQ=255) gACTCTCTAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGAGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGc < 3:241373/148‑1 (MQ=255) tttCGAACCCACCCCTAAACCCATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGAGGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTTGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGt > 2:225372/1‑149 (MQ=255) tttCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGc < 6:209884/61‑1 (MQ=255) tttCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGc > 5:209884/1‑61 (MQ=255) tttCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGAGTAATAACGCTTCGCATGATGGTCGCTTAGGCAAAATGTCGATGCTATCAGAATGGATGAAAGGGGCGTAGAGGGAAAAAGTCTGAAAAGATAACAGGCCAGt > 3:262214/1‑149 (MQ=255) | CAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGT > NC_000913/556349‑556639 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |