Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,112,578 T→C 5.6% A572A (GCT→GCC opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,112,5780TC 5.6% 220.4 / 3.1 71A572A (GCT→GCCopgHOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base C (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCA  >  NC_000913/1112437‑1112726
                                                                                                                                             |                                                                                                                                                    
gctGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCa                                                                                                                                               >  7:80292/1‑149 (MQ=255)
gctGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCa                                                                                                                                               <  8:322503/149‑1 (MQ=255)
    tgGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATgg                                                                                                                                            <  6:302416/148‑1 (MQ=255)
    tgGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATg                                                                                                                                             <  8:232781/147‑1 (MQ=255)
             ttCCTCTCGCTCTCTACTGCATTTCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCATGTGTTCCCAGTGTGGACGCAGTGGCGTCCTGAGCTGGCGATTCCACTTTTTGCTTCGACCATGGTGCtgttgt                                                                                                                                   <  4:75368/148‑1 (MQ=255)
               ccTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTc                                                                                                                                 <  7:135417/148‑1 (MQ=255)
                 tCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTg                                                                                                                              >  4:285794/1‑149 (MQ=255)
                 tCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTg                                                                                                                              >  6:328038/1‑149 (MQ=255)
                       tctctACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAg                                                                                                                        >  1:367738/1‑149 (MQ=255)
                         tctACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGt                                                                                                                       >  6:104114/1‑148 (MQ=255)
                           tACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAt                                                                                                                    >  1:363411/1‑149 (MQ=255)
                             cTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTa                                                                                                                     <  5:215725/146‑1 (MQ=255)
                             cTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   <  3:286272/148‑1 (MQ=255)
                             cTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   <  6:340079/148‑1 (MQ=255)
                             cTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   <  2:367738/148‑1 (MQ=255)
                             cTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   <  2:363411/148‑1 (MQ=255)
                              tGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCCTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   >  4:182803/1‑147 (MQ=255)
                                   tGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAtt                                                                                                            >  2:263259/1‑149 (MQ=255)
                                    gCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAtt                                                                                                            <  3:285794/148‑1 (MQ=255)
                                     cAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAttt                                                                                                           <  5:255915/148‑1 (MQ=255)
                                              gaTGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTCGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACATTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCt                                                                                                  <  8:321993/147‑1 (MQ=255)
                                               aTGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   >  7:1809/1‑130 (MQ=255)
                                               aTGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAtt                                                                                                                   <  8:1809/130‑1 (MQ=255)
                                                tGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGt                                                                                               <  3:297063/149‑1 (MQ=255)
                                                   gTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTgg                                                                                             >  1:349065/1‑147 (MQ=255)
                                                   gTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGc                                                                                             >  4:331118/1‑148 (MQ=255)
                                                   gTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGGTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGt                                                                                                                       <  4:174705/122‑1 (MQ=255)
                                                      gACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCaaa                                                                                          <  5:328038/148‑1 (MQ=255)
                                                      gACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGGGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCaaa                                                                                          <  3:256952/148‑1 (MQ=255)
                                                         cGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGaa                                                                                      >  2:67383/1‑149 (MQ=255)
                                                           aaCCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACg                                                                                    <  8:225990/149‑1 (MQ=255)
                                                              cGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGa                                                                                   >  6:196064/1‑147 (MQ=255)
                                                                     cTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcg                                                                          <  3:57809/149‑1 (MQ=255)
                                                                     cTTCCTGCAACCACGGCAGTTGTTCCCAGTGGGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGTTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGAAAAGGAACGAAAGAATAc                                                                           <  5:225405/148‑1 (MQ=255)
                                                                               ccACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTg                                                                >  4:127519/1‑149 (MQ=255)
                                                                               ccACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTg                                                                <  5:104114/149‑1 (MQ=255)
                                                                                cACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcggcgg                                                                      <  3:58040/142‑1 (MQ=255)
                                                                                cACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcggcgg                                                                      >  4:58040/1‑142 (MQ=255)
                                                                                 aCGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTGTTGCTTCGACCATGCTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGgc                                                              >  5:5508/1‑149 (MQ=255)
                                                                                       gttgttCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCCTGGGGCTGTTTTTCCGGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTGCTGGCGCGTTa                                                        >  6:180686/1‑149 (MQ=255)
                                                                                        ttgttCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTATGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTa                                                        >  7:86335/1‑148 (MQ=255)
                                                                                            tCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGGATTTTGCTTATGTGGTGCAAAGGAAGGAAAGAATACGGCGGCTTCTGGCGCGTTACATTa                                                   >  3:183297/1‑149 (MQ=255)
                                                                                             cccAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTa                                                   <  3:331118/148‑1 (MQ=255)
                                                                                              ccAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTAt                                                  <  3:332418/148‑1 (MQ=255)
                                                                                               cAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCg                                                >  8:218675/1‑149 (MQ=255)
                                                                                               cAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCCTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCg                                                >  1:73113/1‑149 (MQ=255)
                                                                                                aGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGt                                               >  3:45763/1‑149 (MQ=255)
                                                                                                 gtgtGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCCTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGtt                                              <  2:73113/149‑1 (MQ=255)
                                                                                                    tgGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTtgct                                           >  4:199336/1‑149 (MQ=255)
                                                                                                        cGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGctgctg                                       >  7:280958/1‑149 (MQ=255)
                                                                                                          cAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGtt                                              >  6:50244/1‑140 (MQ=255)
                                                                                                          cAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGtt                                              <  5:50244/140‑1 (MQ=255)
                                                                                                           aGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTgg                                      >  2:212628/1‑147 (MQ=255)
                                                                                                           aGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGaa                                    <  1:67383/149‑1 (MQ=255)
                                                                                                               gCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGc                                >  7:92713/1‑149 (MQ=255)
                                                                                                                cGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCt                               <  6:266253/149‑1 (MQ=255)
                                                                                                                    cTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCtttt                            <  3:199336/148‑1 (MQ=255)
                                                                                                                     tGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTTCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGTAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCtttttt                          >  1:325977/1‑149 (MQ=255)
                                                                                                                     tGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCtttttt                          >  4:327145/1‑149 (MQ=255)
                                                                                                                       aGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCTAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCtttttt                          <  7:218675/147‑1 (MQ=255)
                                                                                                                       aGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTcc                        >  6:104667/1‑149 (MQ=255)
                                                                                                                         cTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGt                      >  5:184345/1‑149 (MQ=255)
                                                                                                                          tGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGtg                     >  6:352164/1‑149 (MQ=255)
                                                                                                                          tGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGtg                     >  3:39149/1‑149 (MQ=255)
                                                                                                                            gCGATTGCACTTTTTGCCTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGtg                     <  3:182803/147‑1 (MQ=255)
                                                                                                                              gATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGtgctgc                 >  7:109766/1‑149 (MQ=255)
                                                                                                                                      tttttGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGtgc                    <  8:60004/138‑1 (MQ=255)
                                                                                                                                      tttttGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGtgc                    >  7:60004/1‑138 (MQ=255)
                                                                                                                                      tttttGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCg         >  8:51931/1‑149 (MQ=255)
                                                                                                                                        tttGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCgg        <  8:92713/148‑1 (MQ=255)
                                                                                                                                         ttGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGt       <  5:196064/148‑1 (MQ=255)
                                                                                                                                             ttCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCa  >  7:66831/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCGCGTTACATTATCGTTGCTGCTGGAAGTGCTTTTTTCCGTGCTGCTGGCTCCGGTACGCA  >  NC_000913/1112437‑1112726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: