Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,409,215 G→A 5.5% P118P (CCG→CCA)  yfbR → 5'‑nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,409,2150GA 5.5% 229.0 / 2.3 73P118P (CCG→CCA) yfbR5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base G (37/31);  new base A (2/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

CCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCT  >  NC_000913/2409070‑2409361
                                                                                                                                                 |                                                                                                                                                  
ccTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGtt                                                                                                                                                  >  3:226612/1‑148 (MQ=255)
ccTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGtt                                                                                                                                                  >  1:234978/1‑148 (MQ=255)
  tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAAt                                                                                                                                               >  8:179270/1‑149 (MQ=255)
     gAAGTGCTCACCGGCGATCTCCCTTCTCCGGTGAAATTCTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCTCCTTTAATTGa                                                                                                                                            <  2:72669/149‑1 (MQ=255)
     gAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGa                                                                                                                                            <  5:23322/149‑1 (MQ=255)
         tGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAg                                                                                                                                        <  3:28902/149‑1 (MQ=255)
            tCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcat                                                                                                                                     >  7:165067/1‑149 (MQ=255)
            tCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcat                                                                                                                                     >  8:121972/1‑149 (MQ=255)
             cACCGGAGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatg                                                                                                                                    >  2:191086/1‑149 (MQ=255)
                     aTCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCATTAATTGACGAGCATGCATATAGc                                                                                                                            >  2:138829/1‑149 (MQ=255)
                     aTCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCATTAATTGACGAGCATGCATATAGc                                                                                                                            >  2:144064/1‑149 (MQ=255)
                          ccTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAg                                                                                                                             <  7:200541/143‑1 (MQ=255)
                          ccTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGAt                                                                                                                         <  7:299326/147‑1 (MQ=255)
                          ccTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATg                                                                                                                        >  8:178061/1‑148 (MQ=255)
                                 cGGTTAAATTCTTCAATTCGCATATCGCTCAGGAGTACAAGTCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGCTATGGTTCCGGAAGAGCTGCGGGATATCTTTGCTCCGTTAATTGACGAGCATGCATATAGCGATGAAGaaa                                                                                                                  <  2:357712/147‑1 (MQ=255)
                                 cGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGaaaaa                                                                                                                <  8:270602/149‑1 (MQ=255)
                                 cGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGaaaaa                                                                                                                <  2:262647/149‑1 (MQ=255)
                                 cGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGaaaa                                                                                                                 <  1:191086/148‑1 (MQ=255)
                                      aaaTACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGc                                                                                                            <  4:226612/148‑1 (MQ=255)
                                       aaTACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTg                                                                                                          <  5:323416/149‑1 (MQ=255)
                                            ttCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGa                                                                                                      >  5:38066/1‑148 (MQ=255)
                                            ttCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGa                                                                                                      >  8:285533/1‑148 (MQ=255)
                                            ttCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTCAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAAATGACGAGCATGAATATAGCGATGAAGAAAAATCGATGTTGa                                                                                                      >  1:272807/1‑148 (MQ=255)
                                                aTTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAg                                                                                                 <  3:168500/149‑1 (MQ=255)
                                                    gCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAgg                                                                                                >  7:252618/1‑146 (MQ=255)
                                                    gCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAgg                                                                                                <  2:211649/146‑1 (MQ=255)
                                                    gCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAgg                                                                                                >  1:211649/1‑146 (MQ=255)
                                                    gCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCa                                                                                              <  3:73863/148‑1 (MQ=255)
                                                             tCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAgg                                                                                                >  2:359989/1‑137 (MQ=255)
                                                             tCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAgg                                                                                                <  1:359989/137‑1 (MQ=255)
                                                                      cAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATc                                                                           <  1:325248/149‑1 (MQ=255)
                                                                       aaGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGa                                                                                                                          <  4:224861/101‑1 (MQ=255)
                                                                       aaGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCt                                                                          >  4:101870/1‑149 (MQ=255)
                                                                        aGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTg                                                                         <  3:218372/149‑1 (MQ=255)
                                                                         ggCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAACCAGGCAGATGCACTGTGTGCATATCTg                                                                         >  8:349550/1‑148 (MQ=255)
                                                                         ggCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTg                                                                         >  5:1077/1‑148 (MQ=255)
                                                                             aTTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGa                                                                                                                          >  5:221505/1‑95 (MQ=255)
                                                                             aTTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGa                                                                                                                          <  6:221505/95‑1 (MQ=255)
                                                                             aTTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATg                                                                    >  8:360568/1‑149 (MQ=255)
                                                                                gAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTc                                                                  <  3:101870/148‑1 (MQ=255)
                                                                                 aaaaaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGTAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTTGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCt                                                                 <  3:129197/148‑1 (MQ=255)
                                                                                 aaaaaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCATTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTg                                                                <  1:144064/149‑1 (MQ=255)
                                                                                 aaaaaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCATTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTg                                                                <  1:138829/149‑1 (MQ=255)
                                                                                  aaaaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTgg                                                               <  7:178061/149‑1 (MQ=255)
                                                                                    aaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGgaa                                                             <  8:320910/149‑1 (MQ=255)
                                                                                      aTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGgaag                                                            >  5:86345/1‑148 (MQ=255)
                                                                                           tCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTcg                                                      >  1:43876/1‑149 (MQ=255)
                                                                                             aGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCt                                                                 <  1:319526/136‑1 (MQ=255)
                                                                                             aGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCt                                                                 >  2:319526/1‑136 (MQ=255)
                                                                                             aGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTcgcg                                                    >  1:7803/1‑149 (MQ=255)
                                                                                              gCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCgg                                                   >  3:171646/1‑149 (MQ=255)
                                                                                                aaaaaCTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATATTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACATGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTTGCGGcc                                                 >  3:41848/1‑149 (MQ=255)
                                                                                                      tGGTCGATATGGTTCCGGAAGAGCTGCGAGAAATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGAACTGTGTGCATATCTGAAATGTCTGGCAGAACACCCCGCCGGaaa                                            >  2:282762/1‑148 (MQ=255)
                                                                                                         tCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAaataat                                        >  7:153577/1‑149 (MQ=255)
                                                                                                            atatGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGaa                                     <  7:121972/149‑1 (MQ=255)
                                                                                                                 gTTCCGTAAGAGATGCGGGATATCTTTGAGCCGTTAATTGAAGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAAAAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTTGCGGCCGGAAATAATGAattatt                                <  4:41848/149‑1 (MQ=255)
                                                                                                                       gAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGAt                                                                                           <  8:36494/84‑1 (MQ=255)
                                                                                                                       gAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGAt                                                                                           >  7:36494/1‑84 (MQ=255)
                                                                                                                             cTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAAc                    <  8:252618/149‑1 (MQ=255)
                                                                                                                                cGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAAcgc                  <  6:86345/148‑1 (MQ=255)
                                                                                                                                 gggATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGa                >  6:347691/1‑149 (MQ=255)
                                                                                                                                 gggATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGa                <  4:171646/149‑1 (MQ=255)
                                                                                                                                   gATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACt              >  3:5110/1‑149 (MQ=255)
                                                                                                                                    atatCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTg             >  5:152794/1‑149 (MQ=255)
                                                                                                                                         tttGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGa                                      >  7:283074/1‑119 (MQ=255)
                                                                                                                                         tttGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGa                                      <  8:283074/119‑1 (MQ=255)
                                                                                                                                         tttGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGc        >  2:230886/1‑149 (MQ=255)
                                                                                                                                         tttGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGc        >  1:149069/1‑149 (MQ=255)
                                                                                                                                           tGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGaa          <  7:179270/145‑1 (MQ=255)
                                                                                                                                             cgcCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACg    >  4:40550/1‑149 (MQ=255)
                                                                                                                                              gcCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGc   >  6:226320/1‑149 (MQ=255)
                                                                                                                                              gcCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGc   >  1:260259/1‑149 (MQ=255)
                                                                                                                                               ccGTTAATTGCCGAGCATGCATATAGCGATGAAGAAAAATCTCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAAGTCTTGCGGGCAAACACGCGACTGGCAGCGACGCt  >  6:285547/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCAAAAACGCGACTGGAAGCGACGCT  >  NC_000913/2409070‑2409361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑