Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,972,260 G→C 5.7% V222L (GTC→CTC)  tas → putative NADP(H)‑dependent aldo‑keto reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,972,2600GC 5.7% 241.6 / 5.8 70V222L (GTC→CTC) tasputative NADP(H)‑dependent aldo‑keto reductase
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base C (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

TTCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAA  >  NC_000913/2972114‑2972404
                                                                                                                                                  |                                                                                                                                                
ttCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt                                                                                                                                                 >  7:69408/1‑148 (MQ=255)
  cGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCa                                                                                                                                               <  6:318995/148‑1 (MQ=255)
    ttATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTcctc                                                                                                                                             >  2:169198/1‑145 (MQ=255)
     tataTCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGc                                                                                                                                             >  8:115978/1‑147 (MQ=255)
      atatCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGAAAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCa                                                                                                                                           <  4:42057/148‑1 (MQ=255)
       tatCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGt                                                                                                                                         <  2:70247/149‑1 (MQ=255)
            gCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGaa                                                                                                                                    <  7:69458/149‑1 (MQ=255)
            gCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGaa                                                                                                                                    <  5:179400/149‑1 (MQ=255)
                    aacgaaACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAACTCAGCCAGTATGAAg                                                                                                                                   >  3:185820/1‑142 (MQ=255)
                    aacgaaACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAACTCAGCCAGTATGAAg                                                                                                                                   <  4:185820/142‑1 (MQ=255)
                    aacgaaACTGCATTTGGCGTAATGCGCTACCTGCAGCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAACTCAGCCAGTATGAAg                                                                                                                                   <  4:190461/142‑1 (MQ=255)
                    aacgaaACTGCATTTGGCGTAATGAGCTAACTGCATCTGGCGGACAAACACGATCTGCAGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACAGCAGTTTTGAAGTAGGTCTGGCAGACCTCAGCCAGTATGAAg                                                                                                                                   >  3:190461/1‑142 (MQ=255)
                      cgaaACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAAc                                                                                                                          <  7:115978/149‑1 (MQ=255)
                       gaaACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAAct                                                                                                                         >  8:179568/1‑149 (MQ=255)
                        aaaCTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAActg                                                                                                                        >  1:86510/1‑149 (MQ=255)
                         aaCTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGaa                                                                                                                                    >  6:65186/1‑136 (MQ=255)
                         aaCTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGaa                                                                                                                                    <  5:65186/136‑1 (MQ=255)
                              cATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGc                                                                                                                   <  1:168905/148‑1 (MQ=255)
                              cATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGc                                                                                                                   <  8:69408/148‑1 (MQ=255)
                                  tGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTAt                                                                                                               <  7:179568/148‑1 (MQ=255)
                                    gCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTc                                                                                                             <  7:168606/148‑1 (MQ=255)
                                    gCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCg                                                                                                            <  6:77164/149‑1 (MQ=255)
                                       tAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGc                                                                                                         >  8:327541/1‑149 (MQ=255)
                                        aaTGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAAACCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGcc                                                                                                        >  4:258312/1‑149 (MQ=255)
                                                 ccTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCt                                                                                                       >  6:347239/1‑141 (MQ=255)
                                                 ccTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCt                                                                                                       <  5:347239/141‑1 (MQ=255)
                                                 ccTGCAGCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGTAGTAGGTCTGGCAGAAGGCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCg                                                                                               <  1:169198/149‑1 (MQ=255)
                                                  cTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGt                                                                                                                               <  3:45832/116‑1 (MQ=255)
                                                  cTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGt                                                                                                                               >  4:45832/1‑116 (MQ=255)
                                                      aTCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACg                                                                                          >  1:344574/1‑149 (MQ=255)
                                                              gACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCgg                                                                                  <  2:118126/149‑1 (MQ=255)
                                                                 aaaCACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGa                                                                                >  3:19680/1‑148 (MQ=255)
                                                                   acacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAat                                                                             <  7:265194/149‑1 (MQ=255)
                                                                   acacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTACTGGCATATTCGTGCCTGGGTTTCGGCACGCTGACCGGGaaa                                                                              >  1:208675/1‑148 (MQ=255)
                                                                    cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAata                                                                            <  7:327541/149‑1 (MQ=255)
                                                                     acGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCg                                                                                               >  5:177375/1‑129 (MQ=255)
                                                                     acGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCg                                                                                               <  6:177375/129‑1 (MQ=255)
                                                                       gATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATAtct                                                                         >  2:257735/1‑149 (MQ=255)
                                                                            gCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGcc                                                                                                                  >  7:106470/1‑103 (MQ=255)
                                                                            gCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGcc                                                                                                                  <  8:106470/103‑1 (MQ=255)
                                                                              cgcgTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTACTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGt                                                                  <  2:208675/149‑1 (MQ=255)
                                                                                cgTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTg                                                                 >  6:89624/1‑148 (MQ=255)
                                                                                       tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAAccc                                                         >  1:12848/1‑149 (MQ=255)
                                                                                         aCCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGc                                                       >  4:304250/1‑149 (MQ=255)
                                                                                         aCCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGc                                                       <  4:19680/149‑1 (MQ=255)
                                                                                          ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCt                                                      >  3:203735/1‑149 (MQ=255)
                                                                                           cATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTg                                                     >  4:16604/1‑149 (MQ=255)
                                                                                           cATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTAGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGCGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCCGGAAATATCTCAATGGTGCAAAACCCGCTg                                                     >  7:97741/1‑149 (MQ=255)
                                                                                                aGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCa                                                >  3:127908/1‑149 (MQ=255)
                                                                                                 gAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAatat                                                                           <  3:69778/121‑1 (MQ=255)
                                                                                                 gAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAatat                                                                           >  4:69778/1‑121 (MQ=255)
                                                                                                  aaCCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACg                                              >  3:306335/1‑149 (MQ=255)
                                                                                                  aaCCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGACCGCTGACCGGGAAATATCTCAATGGTGCACAACCCGCTGGCGCACg                                              >  6:4474/1‑149 (MQ=255)
                                                                                                       ttACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATa                                         >  6:273816/1‑149 (MQ=255)
                                                                                                         aCAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATa                                         <  3:258312/147‑1 (MQ=255)
                                                                                                               tGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCtt                                  >  2:42047/1‑148 (MQ=255)
                                                                                                                 ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGt                                                                  <  5:19029/114‑1 (MQ=255)
                                                                                                                 ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGt                                                                  >  6:19029/1‑114 (MQ=255)
                                                                                                                 ttAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCtt                                  <  2:86510/146‑1 (MQ=255)
                                                                                                                   aaaCCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGt                              <  2:344574/148‑1 (MQ=255)
                                                                                                                   aaaCCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGt                              <  3:304250/148‑1 (MQ=255)
                                                                                                                   aaaCCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGCTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCAGCTGGCGCACGTAATACGCTCTTTAGTc                             >  6:264509/1‑149 (MQ=255)
                                                                                                                   aaaCCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCACTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTc                             >  8:113026/1‑149 (MQ=255)
                                                                                                                    aaCCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCg                            >  8:231811/1‑149 (MQ=255)
                                                                                                                        gCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGtt                         >  8:270965/1‑148 (MQ=255)
                                                                                                                           gTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCAcc                     <  4:306335/149‑1 (MQ=255)
                                                                                                                             tttGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGCCCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCg                   >  8:170796/1‑149 (MQ=255)
                                                                                                                                       gTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGt         <  5:89624/149‑1 (MQ=255)
                                                                                                                                       gTCTGGCAGAAGTCAGCCAGGATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGt         <  1:216938/149‑1 (MQ=255)
                                                                                                                                              aGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCaaa  >  2:334420/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTCGTTATATCGGCGTGTCGAACGAAACTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGTCGAACTGCTGGCCTATTCGTGCCTGGGTTTCGGCACGCTGACCGGGAAATATCTCAATGGTGCAAAACCCGCTGGCGCACGTAATACGCTCTTTAGTCGGTTCACCCGCTATAGCGGTGAGCAAA  >  NC_000913/2972114‑2972404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑