Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,335,153	Δ593 bp		[ymiA]–yciX	[ymiA], yciX

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1335153	1335745	593	53 [0]	[0] 51	[ymiA]–yciX	[ymiA],yciX

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1335152	0 (0.000)	51 (0.570)	45/282	0.9	100%	coding (29/165 nt)	ymiA	uncharacterized protein
	?	NC_000913	1335746 =	0 (0.000)					intergenic (+288/‑85)	yciX/acnA	uncharacterized protein/aconitate hydratase 1; aconitase A

CTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCA  >  NC_000913/1335601‑1335892                                                                                                                                                  |                                                                                                                                                   aggtcaaaagattgaattattcactggagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAAt                                                                                                                                                 >  5:336896‑M2/146‑149 (MQ=255)             tgaattattcactggagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagagatggcgattatatgagAAATCGTTACGCGATg                                                                                                                                     >  8:37391‑M2/134‑149 (MQ=255)                   attcactggagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTtgt                                                                                                                                <  5:248638‑M2/21‑1 (MQ=255)                    ttcactggagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTtgtg                                                                                                                               <  5:210493‑M2/22‑1 (MQ=255)                       actggagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAGTCGTTGCGGGAGGgttgtgtgtg                                                                                                                           >  6:265069‑M2/124‑146 (MQ=255)                           gagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatttgagAAATCGTTACGCGATGTTTGTGTTATCtt                                                                                                                        >  4:214791‑M2/120‑148 (MQ=255)                           gagacgattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCtt                                                                                                                        <  6:336896‑M2/29‑1 (MQ=255)                             gacgattcgtaaactcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTaa                                                                                                                     <  7:198610‑M2/32‑1 (MQ=255)                                gattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAatat                                                                                                                  >  5:146695‑M2/115‑149 (MQ=255)                                gattcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAatat                                                                                                                  >  8:37557‑M2/115‑149 (MQ=255)                                   tcgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCa                                                                                                               >  8:348519‑M2/112‑149 (MQ=255)                                    cgtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCa                                                                                                               <  1:382336‑M2/38‑1 (MQ=255)                                     gtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGt                                                                                                                                    <  3:222525‑M2/17‑1 (MQ=255)                                     gtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGt                                                                                                                                    >  4:222525‑M2/110‑126 (MQ=255)                                     gtaaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAcc                                                                                                             >  8:195189‑M2/110‑149 (MQ=255)                                       aaattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCt                                                                                                           >  5:297039‑M2/108‑149 (MQ=255)                                        aattcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCt                                                                                                           <  7:195189‑M2/42‑1 (MQ=255)                                         attcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGa                                                                                                         <  5:265069‑M2/44‑1 (MQ=255)                                          ttcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGaa                                                                                                        <  7:348519‑M2/45‑1 (MQ=255)                                          ttcgctggatttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGaa                                                                                                        <  5:298906‑M2/45‑1 (MQ=255)                                                   tttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacttcttgctgataagtgatggcgattatatgtgAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAg                                                                                               <  5:130243‑M2/54‑1 (MQ=255)                                                   tttttgactaaagtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAg                                                                                               <  7:127414‑M2/54‑1 (MQ=255)                                                              agtttctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAc                                                                                    <  6:297039‑M2/65‑1 (MQ=255)                                                                  tctttaggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCt                                                                                 <  1:320964‑M2/68‑1 (MQ=255)                                                                      taggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGt                                                                               <  8:247223‑M2/70‑1 (MQ=255)                                                                      taggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCa                                                                             <  6:146695‑M2/72‑1 (MQ=255)                                                                       aggatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTTATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt                                                                            >  2:309340‑M2/76‑148 (MQ=255)                                                                         gatttgtctcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGAGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAtt                                                                           >  1:105894‑M2/74‑147 (MQ=255)                                                                                 tcatcgtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGc                                                                 >  7:174695‑M2/66‑149 (MQ=255)                                                                                      gtatgattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATgt                                                            >  4:57676‑M2/61‑149 (MQ=255)                                                                                          gattaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATgtcgt                                                         >  4:88163‑M2/57‑148 (MQ=255)                                                                                            ttaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATgtc                                                           <  1:321407‑M2/90‑1 (MQ=255)                                                                                            ttaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATgtc                                                           >  2:321407‑M2/55‑144 (MQ=255)                                                                                            ttaattacaccaatcgttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCaa                                                      >  2:14051‑M2/55‑149 (MQ=255)                                                                                                       aatcgttcaacgtcttgctgatatgtgatggcgattatatgagATATCGTTTCGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCAGTCATTAAGGAGGAGCTATGTCGTCAACCCTACgag                                             <  8:101530‑M2/104‑1 (MQ=255)                                                                                                       aatcgttcaacctcttgctgataagtgatggcgattatatgagAAATCGTTACGCGAAGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGTAGCTATGTCGTCAACCCTACgag                                             >  7:101530‑M2/44‑147 (MQ=255)                                                                                                            ttcaacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTa                                                               <  2:193723‑M2/86‑1 (MQ=255)                                                                                                               aacgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGt                                     >  2:133490‑M2/36‑147 (MQ=255)                                                                                                                acgtcttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAg                                  <  1:133490‑M2/115‑1 (MQ=255)                                                                                                                     ttgctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTACACCATACGAGAAGCCAGTAAGGacac                             >  4:325445‑M2/30‑149 (MQ=255)                                                                                                                       gctgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGt                           >  1:147597‑M2/28‑149 (MQ=255)                                                                                                                         tgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTg                         >  6:170717‑M2/26‑149 (MQ=255)                                                                                                                         tgataagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTg                         >  2:308652‑M2/26‑149 (MQ=255)                                                                                                                           ataagtgatggagattatatgatAAATCGTTACGCGATGTTTGTTTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTg                         <  2:105894‑M2/124‑1 (MQ=255)                                                                                                                            taagtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAg                      <  7:37557‑M2/127‑1 (MQ=255)                                                                                                                              agtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGc                    <  3:88163‑M2/129‑1 (MQ=255)                                                                                                                              agtgatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGc                    >  4:75328‑M2/21‑149 (MQ=255)                                                                                                                                 gatggcgattatatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCa                  <  1:309340‑M2/131‑1 (MQ=255)                                                                                                                                            tatgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACt       <  1:308652‑M2/142‑1 (MQ=255)                                                                                                                                             atgagAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTa     >  1:344488‑M2/6‑149 (MQ=255)                                                                                                                                                agAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTAGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGCTAAAACTtcccc  >  4:57735‑M2/3‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCA  >  NC_000913/1335601‑1335892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑