| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,354,596 | C→T | 6.6% | G292D (GGC→GAC) | sapB ← | antimicrobial peptide transport ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,354,596 | 0 | C | T | 6.6% | 185.2 / 2.6 | 60 | G292D (GGC→GAC) | sapB | antimicrobial peptide transport ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (26/30); new base T (2/2); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCG > NC_000913/1354449‑1354741 | aaaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGc > 5:43572/1‑148 (MQ=255) tGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGCGCcacaac > 6:22373/1‑146 (MQ=255) gcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCa < 6:43572/148‑1 (MQ=255) aGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCCTGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCa > 3:138137/1‑148 (MQ=255) ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATc > 4:272778/1‑146 (MQ=255) ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATc < 3:272778/146‑1 (MQ=255) aGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaa < 6:327210/148‑1 (MQ=255) cGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAt < 7:435564/148‑1 (MQ=255) ttttCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGTCACACACCATCACTCCTGCGGaa > 1:199835/1‑130 (MQ=255) ttttCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGTCACACACCATCACTCCTGCGGaa < 2:199835/130‑1 (MQ=255) ttttCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGTCACACACCATCACTCCGGCGGaa < 2:205927/130‑1 (MQ=255) ttttCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGTCACACACCATCACTCCAGCGGaa > 1:205927/1‑130 (MQ=255) tttCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctgct > 3:2180/1‑148 (MQ=255) ttCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCATAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctgctg > 6:233648/1‑148 (MQ=255) cTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCg > 7:173089/1‑147 (MQ=255) tataCGCTTTAGTTAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAGCCAAAATATCATAAATAAAGTTAACAATAATGACCAGTTAGCCACTCACAATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGa < 6:426936/146‑1 (MQ=255) tataCGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATTACGTTAACAATAATGACCAGTGAGCCACACACCATCACt > 8:445823/1‑112 (MQ=255) tataCGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATTACGTTAACAATAATGACCAGTGAGCCACACACCATCACt < 7:445823/112‑1 (MQ=255) tataCGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATg < 8:192488/148‑1 (MQ=255) tataCGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGGGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGa < 3:140152/146‑1 (MQ=255) aCGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCTTGCTGGCGGATGGCg > 8:424304/1‑148 (MQ=255) cGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCg < 6:91159/147‑1 (MQ=255) aTCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCAc < 2:169064/103‑1 (MQ=255) aTCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCAc > 1:169064/1‑103 (MQ=255) gTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGCTCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCATGCTGGCGGATGGCGttaattaa > 3:243004/1‑148 (MQ=255) gTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaatta < 4:138137/147‑1 (MQ=255) gCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAgc < 5:22373/148‑1 (MQ=255) aCCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaa < 3:208342/101‑1 (MQ=255) aCCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaa > 4:208342/1‑101 (MQ=255) aCCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaattaa < 4:169108/139‑1 (MQ=255) aCCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaattaa > 3:169108/1‑139 (MQ=255) aCCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGtta < 7:299591/134‑1 (MQ=255) aCCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGtta > 8:299591/1‑134 (MQ=255) ataTTCCTTATGTTTCAGAGGTTTATCCATGGCACACAAAATTTCAGAAATCACGTTAACAATAATGACCAGTGAGCCAGACACCATAACTCCGGCGGATATGGCTGCGTTTTCCTGCTGGCGGATGGCGTTATTTAAGCAGCGccg < 7:79636/145‑2 (MQ=255) cATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATAAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTTCTTAATCCTGCTGGCGGATGGCTTTAATTAACCAGAGccat > 4:253354/1‑145 (MQ=255) ttCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTaaa > 1:269285/1‑148 (MQ=255) cTTATGTTTCAGAGGGTTAGCAATGGCACCCAAAATATAAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGAGGATGGCGTTAATTAACCAGCGCCCTAAAcc < 3:253354/147‑1 (MQ=255) ttATGTTTCAGAGGGTAAGCCATGCCACCCAAAATATCAGAAATCACGTAAACAATAATGACCAGAGAGCCAAAGACCATCCCTCCGTCGTCAAAGGCTACCTAATCCTCCTGGCCCCACGCGGAAATTAACAAGCGCCCTaacaccg > 4:285394/1‑143 (MQ=255) tATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCgg > 2:269312/1‑148 (MQ=255) tttCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCAGCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAg < 3:72023/148‑1 (MQ=255) gCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCAtt < 5:233648/147‑1 (MQ=255) tGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGt < 8:173089/148‑1 (MQ=255) tGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGt > 4:9651/1‑148 (MQ=255) cccAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACCCCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTACACCCGGCCAGCTAAAACCCATTTCGGTGatca > 1:396015/1‑148 (MQ=255) aaTATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCAtt > 4:186329/1‑133 (MQ=255) aaTATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCAtt < 3:186329/133‑1 (MQ=255) atCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGc > 7:391833/1‑63 (MQ=255) atCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGc < 8:391833/63‑1 (MQ=255) tCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCg > 8:447056/1‑148 (MQ=255) tCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCTTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCAt < 7:424304/148‑1 (MQ=255) cACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGc > 5:413099/1‑145 (MQ=255) cACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGc < 6:413099/145‑1 (MQ=255) aCGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCAtg < 2:269285/147‑1 (MQ=255) cGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCAtggt > 7:52497/1‑148 (MQ=255) aaCAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGATAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGa > 7:228/1‑147 (MQ=255) aCAATAATGACCTTTTAGCCACACTGCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATTGCTTTAATTAACCAGCTCACTAAACCCTGCCAGCTAAAAACGATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGaaa < 2:403348/148‑1 (MQ=255) ataatGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaatta < 5:167219/76‑1 (MQ=255) ataatGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGttaatta > 6:167219/1‑76 (MQ=255) aCCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGc < 1:269312/148‑1 (MQ=255) cAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGccc < 8:52497/148‑1 (MQ=255) gCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCg < 3:9651/147‑1 (MQ=255) | AAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCG > NC_000913/1354449‑1354741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |