Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,265 C→T 6.8% P70S (CCC→TCC)  ydbJ → DUF333 family putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,2650CT 6.8% 184.8 / 2.8 59P70S (CCC→TCC) ydbJDUF333 family putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (31/24);  new base T (2/2);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

TTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTTGTGCACCT  >  NC_000913/1441122‑1441411
                                                                                                                                               |                                                                                                                                                  
ttCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCaa                                                                                                                                                >  8:334902/1‑148 (MQ=255)
ttCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTATTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCaa                                                                                                                                                >  8:299253/1‑148 (MQ=255)
      agGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCa                                                                                                                                           >  7:294504/1‑147 (MQ=255)
               ccGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTTGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTa                                                                                                                                  <  4:77594/147‑1 (MQ=255)
                cgcgCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTATGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGc                                                                                                                                >  7:50519/1‑148 (MQ=255)
                cgcgCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGc                                                                                                                                >  7:154510/1‑148 (MQ=255)
                    cACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAAc                                                                                                                            <  6:293372/148‑1 (MQ=255)
                        tAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTc                                                                                                                        <  2:56049/148‑1 (MQ=255)
                          gcgcCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCa                                                                                                                       <  4:301068/147‑1 (MQ=255)
                               aGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTAAACTGGATGGTACGGCGATTGGGATGTGTGAATTACCCAACGGCAAACGCTGTAGCGAACAGTCCCTTgcc                                                                                                                 >  2:203897/1‑148 (MQ=255)
                                 gTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCGGTAGCGAACAGTCACTTgccgc                                                                                                               <  3:116145/148‑1 (MQ=255)
                                  tttAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCa                                                                                                                       <  1:164121/139‑1 (MQ=255)
                                  tttAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCa                                                                                                                       >  2:164121/1‑139 (MQ=255)
                                  tttAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTgccgcc                                                                                                              >  5:186227/1‑148 (MQ=255)
                                                tGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCtg                                                                                                      >  4:105813/1‑142 (MQ=255)
                                                tGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCtg                                                                                                      <  3:105813/142‑1 (MQ=255)
                                                    cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTATCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTAt                                                                                            >  8:147444/1‑148 (MQ=255)
                                                    cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTATCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCAGGGAGCAGTGGCAGCTa                                                                                             >  6:197570/1‑147 (MQ=255)
                                                    cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCa                                                                                                                       >  5:313878/1‑121 (MQ=255)
                                                    cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCa                                                                                                                       <  6:313878/121‑1 (MQ=255)
                                                    cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTa                                                                                             <  8:294504/147‑1 (MQ=255)
                                                    cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCAATTGGGATGTGTGCATTACCCAACGGAAAACCCTGTAGCGAACAGTCACATCCCGCCGGGAGCTGTGGCAGCTAt                                                                                            >  4:291266/1‑148 (MQ=255)
                                                       tagtGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTaa                                                                                         <  1:255047/148‑1 (MQ=255)
                                                        agtGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAg                                                                                                                          >  6:185363/1‑114 (MQ=255)
                                                        agtGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAg                                                                                                                          <  5:185363/114‑1 (MQ=255)
                                                           ggAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCa                                                                                     >  4:52627/1‑148 (MQ=255)
                                                            gagaAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCa                                                                                     <  7:334902/147‑1 (MQ=255)
                                                                  cAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAg                                                                                                                                 <  7:280877/97‑1 (MQ=255)
                                                                  cAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAg                                                                                                                                 >  8:280877/1‑97 (MQ=255)
                                                                  cAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCGGTAGCGAACAGTCa                                                                                                                       >  3:296817/1‑107 (MQ=255)
                                                                  cAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCCGTAGCGAACAGTCa                                                                                                                       <  4:296817/107‑1 (MQ=255)
                                                                   aaaTTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCa                                                                                                                                                 <  8:92339/80‑1 (MQ=255)
                                                                   aaaTTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCa                                                                                                                                                 >  7:92339/1‑80 (MQ=255)
                                                                                   ggcggTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTa                                                             <  6:186227/148‑1 (MQ=255)
                                                                                   ggcggTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACATGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAg                                                                <  3:291266/145‑1 (MQ=255)
                                                                                              ttCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGtttt                                                  >  7:360359/1‑148 (MQ=255)
                                                                                                   ttGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTATCCAACGTCAAACGCTGTATCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGc                                                <  5:197570/145‑1 (MQ=255)
                                                                                                   ttGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTATCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGc                                                <  7:147444/145‑1 (MQ=255)
                                                                                                    tGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTc                                            >  8:5827/1‑148 (MQ=255)
                                                                                                          tCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGATCAGTCACTTGCCGGCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGc                                      <  8:154510/148‑1 (MQ=255)
                                                                                                           cAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCg                                     >  4:303460/1‑148 (MQ=255)
                                                                                                              cTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGtt                                   >  3:228791/1‑147 (MQ=255)
                                                                                                              cTGGATGGTACGGCGATTGCGATGTGTGCATTACCCAACGGCAAACGCGGTACCGAAAAGTCACTTGCCGCCGGGAGATGTGGAAGCTATTAATTCATTAAATCCGCCAGGTTATACTTTATTGTCTGGTTTCCGGTCGCCAGCGtt                                   >  2:306107/1‑147 (MQ=255)
                                                                                                                ggATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTTTTAATTCATGAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTaaa                                >  5:227833/1‑147 (MQ=255)
                                                                                                                 gATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTaa                                 <  4:228791/146‑1 (MQ=255)
                                                                                                                                 ggATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGAt                <  7:5827/147‑1 (MQ=255)
                                                                                                                                   aTGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCa             <  3:303460/148‑1 (MQ=255)
                                                                                                                                       gtgCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTtg         >  6:316032/1‑148 (MQ=255)
                                                                                                                                        tgCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTtgt        >  6:206445/1‑148 (MQ=255)
                                                                                                                                        tgCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTtgt        >  4:9739/1‑148 (MQ=255)
                                                                                                                                        tgCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTtgt        >  4:198141/1‑148 (MQ=255)
                                                                                                                                        tgCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTtgt        <  3:386102/148‑1 (MQ=255)
                                                                                                                                             tACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTaaa                                                                                >  6:364386/1‑71 (MQ=255)
                                                                                                                                             tACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTaaa                                                                                <  5:364386/71‑1 (MQ=255)
                                                                                                                                             tACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACtt          >  2:2087/1‑141 (MQ=255)
                                                                                                                                             tACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACtt          <  1:2087/141‑1 (MQ=255)
                                                                                                                                             tACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTTGTGCAcc   >  5:413068/1‑148 (MQ=255)
                                                                                                                                             tACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTTGTGCAcc   >  3:1752/1‑148 (MQ=255)
                                                                                                                                              aCCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTTGTGCACct  >  8:318787/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGCCAGCTTATAAGTTAATGTCTGTTTTGCGGTCGCCAGCGTTAACTGGTTCGCGGTCAGATCCACTTGTGCACCT  >  NC_000913/1441122‑1441411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑