Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,610,455 G→A 7.6% L76L (CTG→TTG)  uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,610,4550GA 7.6% 164.4 / 3.3 55L76L (CTG→TTG) uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACA  >  NC_000913/1610317‑1610591
                                                                                                                                          |                                                                                                                                        
tggtgTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGAt                                                                                                                                 <  3:283621/148‑1 (MQ=255)
     ttAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAAtgg                                                                                                                            >  1:279900/1‑148 (MQ=255)
       agagAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAAtggt                                                                                                                           <  2:1449/147‑1 (MQ=255)
         agaAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGCCGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATggtggt                                                                                                                        >  3:98623/1‑148 (MQ=255)
         agaAAACAAAGCGCATTTCCGGGATGTGCCCCACTTTCAGGAATTAATCGTATTCACTGAAGACGCTGATATCACGATTAACCGAGCGAATAAGACGAGCGTCCCTGACAGCATCCCCCTACTCAATCAGGCCACGGATAATggtggt                                                                                                                        >  4:329362/1‑148 (MQ=255)
            aaaCAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATa                                                                                                                                >  5:433556/1‑137 (MQ=255)
            aaaCAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATa                                                                                                                                <  6:433556/137‑1 (MQ=255)
             aaCAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTAc                                                                                                                    <  6:429936/148‑1 (MQ=255)
             aaCAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTAc                                                                                                                    <  1:38455/148‑1 (MQ=255)
                   gcgcATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCa                                                                                                              >  1:47236/1‑148 (MQ=255)
                    cgcATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCAt                                                                                                             >  8:93285/1‑148 (MQ=255)
                      cATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCCGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATcc                                                                                                           >  5:56581/1‑148 (MQ=255)
                         ttCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGAcc                                                                                                               >  2:285248/1‑141 (MQ=255)
                         ttCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGAcc                                                                                                               <  1:285248/141‑1 (MQ=255)
                           ccgtggtgtgCGCCATTTTCAGGACTTCATCGTATTCACTGTAGAGGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTCCCCCCTTCGCATTCACGCCACGGATAATGGTGGTGTACAGACCATCCTGCg                                                                                                       <  6:301218/141‑1 (MQ=255)
                             gggTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCAt                                                                                                             >  6:339638/1‑139 (MQ=255)
                             gggTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCAt                                                                                                             <  5:339638/139‑1 (MQ=255)
                                      gcCAGTTTCAGGAATTCATCGTAGTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGAcg                                                                                           >  2:229178/1‑148 (MQ=255)
                                           tttCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACATACCATCCTGCGTGCTCAGTGAcggcgg                                                                                       >  7:130386/1‑147 (MQ=255)
                                           tttCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAAGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGAcggcgg                                                                                       >  8:171507/1‑147 (MQ=255)
                                           tttCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAAGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGAcggcgg                                                                                       >  8:177681/1‑147 (MQ=255)
                                              cAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAAt                                                                                   >  1:360634/1‑148 (MQ=255)
                                                       aTCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCa                                                                          <  5:157697/148‑1 (MQ=255)
                                                        tCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGa                                                                                     >  6:266830/1‑136 (MQ=255)
                                                        tCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGa                                                                                     <  5:266830/136‑1 (MQ=255)
                                                          gTATTCACTGTAGACGCTGATTTCACGATTAACCGATCGAATCAGTCTCGCGTCGCTGACCGCTTACCCCTTATCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAAt                                                                        <  8:130386/147‑1 (MQ=255)
                                                             ttCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCgg                                                                     <  2:360634/147‑1 (MQ=255)
                                                             ttCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGa                                                                    >  6:305556/1‑148 (MQ=255)
                                                              tCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGAc                                                                   >  2:29038/1‑148 (MQ=255)
                                                                   gTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAAtggt                                                                                                                           >  1:308559/1‑87 (MQ=255)
                                                                   gTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAAtggt                                                                                                                           <  2:308559/87‑1 (MQ=255)
                                                                   gTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTc                                                                                                  >  8:338617/1‑112 (MQ=255)
                                                                   gTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTc                                                                                                  <  7:338617/112‑1 (MQ=255)
                                                                    tAGACGCTGATTTCATGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGaacaa                                                             >  1:253698/1‑148 (MQ=255)
                                                                          cTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGTAGTTTCAATCGGACGAACAACGACCa                                                       <  4:98623/148‑1 (MQ=255)
                                                                               ttCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTAc                                                                                                                    <  5:218686/82‑1 (MQ=255)
                                                                               ttCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTAc                                                                                                                    >  6:218686/1‑82 (MQ=255)
                                                                                 cACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAg                                                 >  1:329900/1‑147 (MQ=255)
                                                                                 cACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAg                                                 >  8:59453/1‑147 (MQ=255)
                                                                                    gATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAAtt                                             <  7:93285/148‑1 (MQ=255)
                                                                                      ttAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAAtt                                             >  8:64742/1‑146 (MQ=255)
                                                                                        aaCCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAg                                          <  2:279900/147‑1 (MQ=255)
                                                                                         aCCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGa                                         <  7:59453/147‑1 (MQ=255)
                                                                                         aCCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAAGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGa                                         <  7:177681/147‑1 (MQ=255)
                                                                                         aCCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAAGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGa                                         <  7:171507/147‑1 (MQ=255)
                                                                                            gAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCgg                                     <  2:329900/148‑1 (MQ=255)
                                                                                            gAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCgg                                     <  1:29038/148‑1 (MQ=255)
                                                                                                     aGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCa                             <  2:253698/147‑1 (MQ=255)
                                                                                                            cgTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCAt                            >  7:58085/1‑141 (MQ=255)
                                                                                                            cgTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCAt                            <  8:58085/141‑1 (MQ=255)
                                                                                                             gTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGgaga                    <  1:229178/148‑1 (MQ=255)
                                                                                                                           cccccTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGt       >  7:320063/1‑147 (MQ=255)
                                                                                                                                 tctcATTCAGGCCACGGATAATGGTGGTGTACAGCCCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTcaaca  >  6:197816/1‑146 (MQ=255)
                                                                                                                                       tCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGAcc                                                        <  3:251275/86‑1 (MQ=255)
                                                                                                                                       tCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGAcc                                                        >  4:251275/1‑86 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACA  >  NC_000913/1610317‑1610591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑