| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,735,899 | T→C | 7.3% | Y174Y (TAT→TAC) | sodB → | superoxide dismutase, Fe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,735,899 | 0 | T | C | 7.3% | 163.5 / 3.5 | 55 | Y174Y (TAT→TAC) | sodB | superoxide dismutase, Fe |
| Reads supporting (aligned to +/- strand): ref base T (25/26); new base C (2/2); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01 | |||||||||||
GTGAAAAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGG > NC_000913/1735756‑1736039 | gTGAAAAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTgg < 3:270911/148‑1 (MQ=255) gAAAAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGCCTCCGCTGCTGCCCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCCCGTCCTGGCTATCTGGAg > 2:396036/1‑148 (MQ=255) gAAAAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAg > 1:328624/1‑148 (MQ=255) aaaaaCAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCAGGAGc > 6:7562/1‑148 (MQ=255) cAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGc > 8:204834/1‑143 (MQ=255) cAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGc < 7:204834/143‑1 (MQ=255) aGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCt < 7:352611/148‑1 (MQ=255) gCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTg < 5:7562/148‑1 (MQ=255) cAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTg < 3:183369/148‑1 (MQ=255) aaCTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGt > 8:13226/1‑148 (MQ=255) cTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTg > 6:4080/1‑148 (MQ=255) cGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGaa > 4:155034/1‑148 (MQ=255) ctctAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGc > 5:144152/1‑147 (MQ=255) ctAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGa > 1:186228/1‑147 (MQ=255) aCGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGaaaa < 8:90043/147‑1 (MQ=255) cTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGc < 8:231548/148‑1 (MQ=255) ccGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCa > 7:153262/1‑147 (MQ=255) cTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGa < 6:135375/112‑1 (MQ=255) cTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGa > 5:135375/1‑112 (MQ=255) accaccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCataata < 1:90123/146‑1 (MQ=255) accaccGATGCGACTACGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCataata < 5:4080/146‑1 (MQ=255) caccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTg < 2:186228/148‑1 (MQ=255) accGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGa < 4:233355/148‑1 (MQ=255) cGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATg > 7:387934/1‑148 (MQ=255) tGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCa < 4:111397/133‑1 (MQ=255) tGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCa > 3:111397/1‑133 (MQ=255) cGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATgcag < 8:387934/147‑1 (MQ=255) ctgctgACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATgcagcct > 1:310482/1‑146 (MQ=255) gctgACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTg > 3:411032/1‑148 (MQ=255) aCCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCAGTTCTGGGCGCTGGGGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGcc < 8:159141/146‑1 (MQ=255) aCCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGcc < 6:144152/146‑1 (MQ=255) acacGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc < 8:168337/96‑1 (MQ=255) acacGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc > 7:168337/1‑96 (MQ=255) attaCATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAAc > 7:218840/1‑99 (MQ=255) attaCATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAAc < 8:218840/99‑1 (MQ=255) taCATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGgcg < 5:68860/51‑1 (MQ=255) taCATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGgcg > 6:68860/1‑51 (MQ=255) aTCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGat > 6:161590/1‑148 (MQ=255) tCGACTATCGCAATGCACGTCCTGGCTACCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGat > 2:132604/1‑147 (MQ=255) aagACTATCCCAATGCACGTCCTGGCTACCTGGAGCACTTCTGGGCGCTGGTGCACTGGGAATTCGTAGCGAAAAATCTCGCTACATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACTCTTATCATGACTACGCGGat > 8:181313/3‑147 (MQ=255) aCTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCTTAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGatcatc > 8:123689/1‑148 (MQ=255) gCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGt < 3:155034/147‑1 (MQ=255) gCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGt < 5:161590/147‑1 (MQ=255) gCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTa < 4:411032/148‑1 (MQ=255) gCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCGGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCg > 8:225738/1‑148 (MQ=255) gCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGat > 6:76958/1‑133 (MQ=255) gCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGat < 5:76958/133‑1 (MQ=255) cGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGAt < 8:153262/148‑1 (MQ=255) cGTCCTGGCTACCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGAt < 7:181313/148‑1 (MQ=255) cGTCCTGGCTACCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGAt < 1:132604/148‑1 (MQ=255) tGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCaa > 1:403269/1‑80 (MQ=255) tGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCaa < 2:403269/80‑1 (MQ=255) tGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTCCGCGGATCATCGATGTAGGTCGGATAAgg > 1:97703/1‑147 (MQ=255) ggCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATc > 2:220462/1‑110 (MQ=255) ggCTATCTGGAGCACTTCTGGGCGCTGGGGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATc < 1:220462/110‑1 (MQ=255) tCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCAtaataa > 4:211768/1‑64 (MQ=255) tCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCAtaataa < 3:211768/64‑1 (MQ=255) | GTGAAAAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGG > NC_000913/1735756‑1736039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |