| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,381,705 | G→A | 6.2% | P321P (CCG→CCA) | degQ → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,381,705 | 0 | G | A | 6.2% | 201.4 / 2.8 | 64 | P321P (CCG→CCA) | degQ | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base G (29/31); new base A (2/2); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
GGCACCGAGATGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGG > NC_000913/3381559‑3381838 | ggCACCGAGATGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGc > 6:321735/1‑148 (MQ=255) cACCGAGATGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTGAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTg < 7:373774/148‑1 (MQ=255) cGAGATGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGaa > 5:287172/1‑147 (MQ=255) gagaTGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATa < 7:318222/148‑1 (MQ=255) aTGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCt < 1:376399/148‑1 (MQ=255) tGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCtt < 4:197906/148‑1 (MQ=255) aGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCATCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCATCGGGAAACCGCTGAATAGCTTTg < 5:368127/148‑1 (MQ=255) aGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTGGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTg < 4:105033/148‑1 (MQ=255) atCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTgc > 1:43996/1‑148 (MQ=255) gCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTgcgc < 5:321735/147‑1 (MQ=255) cAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGctct > 8:333978/1‑148 (MQ=255) aaGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGTTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGa > 1:2924/1‑136 (MQ=255) aaGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGa < 2:2924/136‑1 (MQ=255) gCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTg < 7:151545/133‑1 (MQ=255) gCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTg > 8:151545/1‑133 (MQ=255) ttCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATcg > 3:387523/1‑148 (MQ=255) aaCCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTTCCAGGTTCTGGCTCGGCAAATGCGGGCGTCAAAGCGGGGGATATTATTACGAGCCTCAAGGGCAAACCGCTGATTAGCTTTGCTGAGTTGCGCTCTCGTATcgcg < 3:317318/147‑1 (MQ=255) aaCCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGa < 7:333978/148‑1 (MQ=255) ccTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGacc > 2:360934/1‑148 (MQ=255) cTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGc < 7:370003/109‑1 (MQ=255) cTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGc > 8:370003/1‑109 (MQ=255) cTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGt < 1:10092/127‑1 (MQ=255) cTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGt > 2:10092/1‑127 (MQ=255) gcgcGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGaa < 4:387523/148‑1 (MQ=255) gcgcGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGaa < 7:189702/148‑1 (MQ=255) cgcgTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGcc < 1:208532/138‑1 (MQ=255) cgcgTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGcc > 2:208532/1‑138 (MQ=255) cgcgTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGa > 8:189702/1‑146 (MQ=255) cgTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAATGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGt < 2:43996/148‑1 (MQ=255) gTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTTAAg < 6:287172/147‑1 (MQ=255) tCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCt < 1:360934/148‑1 (MQ=255) gCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTgg < 3:244484/148‑1 (MQ=255) gtTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCctgctg > 7:338192/1‑148 (MQ=255) ttGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCtgctgc > 5:37989/1‑148 (MQ=255) gCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCg > 5:373247/1‑147 (MQ=255) ttCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACgg > 1:27098/1‑147 (MQ=255) tCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAg > 5:367873/1‑81 (MQ=255) tCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAg < 6:367873/81‑1 (MQ=255) tCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGc > 5:209071/1‑147 (MQ=255) gCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCaa < 6:373247/145‑1 (MQ=255) cAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGa < 6:209071/148‑1 (MQ=255) aaGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTa < 8:14155/129‑1 (MQ=255) aaGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTa > 7:14155/1‑129 (MQ=255) aGCGGGCGTCAAAGAGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGATGAGTTGCGATCGCGTATCGCGAACACTGAGCCGGGCGCGAAAGTGAGGCTTGGACTGCTGCGTAACTGCAAACAACTGCAAGTa > 8:233632/1‑148 (MQ=255) cAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGaa < 4:5055/96‑1 (MQ=255) cAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGaa > 3:5055/1‑96 (MQ=255) cAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCACTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGc < 1:14413/104‑1 (MQ=255) cAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCACTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGc < 1:385511/104‑1 (MQ=255) cAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCACTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGc > 2:14413/1‑104 (MQ=255) cAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCACTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGc > 2:385511/1‑104 (MQ=255) aGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCctgc > 6:19779/1‑110 (MQ=255) aGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCctgc < 5:19779/110‑1 (MQ=255) aGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTc < 6:37989/148‑1 (MQ=255) gCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTa > 1:237224/1‑67 (MQ=255) gCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTa < 2:237224/67‑1 (MQ=255) cGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGaa < 2:80991/131‑1 (MQ=255) cGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGaa > 1:80991/1‑131 (MQ=255) atatTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCTCGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCACACCACTGGAAGTAGAAGTGCCGCTCGATACCAg > 3:358183/1‑148 (MQ=255) ccAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTtc > 5:421760/1‑148 (MQ=255) ccAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACAAGCACCTCTtc > 5:358802/1‑148 (MQ=255) cAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCgg < 5:188259/140‑1 (MQ=255) cAAACAGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCgg > 6:188259/1‑140 (MQ=255) ccGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCg < 8:73076/115‑1 (MQ=255) ccGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGAGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCg > 7:73076/1‑115 (MQ=255) | GGCACCGAGATGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTGTCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAACCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCGTAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGG > NC_000913/3381559‑3381838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |