Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,620 | C→T | 5.7% | intergenic (+86/‑110) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,620 | 0 | C | T | 5.7% | 340.7 / 6.2 | 107 | intergenic (+86/‑110) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base C (52/48); new base T (3/3); total (56/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01 |
Only 100 of 109 total aligned reads displayed.
CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGG > NC_000913/164472‑164767 | cATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 8:338200/149‑1 (MQ=255) tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:195930/149‑1 (MQ=255) tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 4:89045/149‑1 (MQ=255) tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTGCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 1:374404/149‑1 (MQ=255) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 4:238538/148‑1 (MQ=255) tCTGGACTGACGACCCGGCAAATACTGCACCGACGCGACGTACTTAAAAGTATTCTTTAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACACTCACCCTAACCCACTCCCTCCAgg < 6:165092/149‑1 (MQ=37) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAgg > 1:116872/1‑149 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 6:294384/149‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 3:323606/149‑1 (MQ=255) acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 7:132135/149‑1 (MQ=255) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc > 5:73320/1‑149 (MQ=255) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTAGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTAACCCACCCCATAACCCTCTCCCGCAAGGGAGAGGGGccc > 7:11060/1‑149 (MQ=37) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 6:160193/149‑1 (MQ=255) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 4:307348/149‑1 (MQ=255) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc > 7:322089/1‑149 (MQ=255) ggCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCGCACCCTAACCCACCACCGCCAGGGAGAGGGGGCCgcacg > 7:224492/1‑145 (MQ=37) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt > 2:155875/1‑149 (MQ=255) cgacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 6:6241/149‑1 (MQ=255) gacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 6:73320/149‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 1:245464/149‑1 (MQ=255) cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat < 7:320693/149‑1 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg > 4:245035/1‑113 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 3:245035/113‑1 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat > 5:133625/1‑148 (MQ=255) acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGGGAGata > 4:117430/1‑148 (MQ=255) acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGata > 2:267977/1‑148 (MQ=255) ggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 8:322089/149‑1 (MQ=255) gAATAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCGCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt < 4:171119/149‑1 (MQ=38) gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGTGCCGGGGTGAGGGCATCAGAGCGCACGTTCCCCCTCCCCCTAACCCTCTCCCGGAAGGGAGGGGGGAACGCTCGCCCACAAATTTTGAGAGATAtcttctt > 7:311030/1‑149 (MQ=37) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc > 7:142423/1‑149 (MQ=255) tATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 5:281327/149‑1 (MQ=255) ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:97093/148‑1 (MQ=255) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCACCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga > 5:284862/1‑124 (MQ=255) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCACCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 6:284862/124‑1 (MQ=255) ggcgggttTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 8:151720/145‑1 (MQ=255) gttggttTTCGCCCGCTCCATGGGGTTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 8:224492/148‑1 (MQ=255) ggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 1:267977/148‑1 (MQ=255) ggttTTCTCCCGCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 5:259648/148‑1 (MQ=255) tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAAt > 2:21882/1‑149 (MQ=255) ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCTCACCCTAACCCTCTCCCGCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATc > 5:410720/1‑149 (MQ=255) ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGCGCGGGGCCCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATc > 5:134811/1‑149 (MQ=255) ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 7:100306/108‑1 (MQ=255) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCg > 4:66395/1‑149 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑ATAATCgg > 1:162873/1‑149 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCgg > 1:312642/1‑149 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAGCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCgg < 2:116872/149‑1 (MQ=255) tctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCggg > 2:82747/1‑149 (MQ=255) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGTGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCttt > 7:116350/1‑149 (MQ=255) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCttt < 6:133625/149‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCtt < 7:17539/142‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCAACCGAACCCTATGCCGCAAGGGCGAGGGGGCCCAAACAGCACAAAATTTGAGAGCTATCTTCATTTGTGTTGGAAAAGAATAACAC‑AAAATAGGGGtttatatag > 8:17539/1‑143 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATAAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGCCCGATCGGGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAATCGGGCTTTTccgc > 1:27534/1‑149 (MQ=255) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGCACCCAAACCCACTCCCGCAAGGGAGAGGGGGCCGATCGCGCCCAAAATTTGAGAGATATATTATTCTGTCTTGTAACAGAAGAACAG‑AAAATAGGGGGTTTTCGCCGGa > 6:257763/1‑148 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGCGCACACATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGaa > 5:91620/1‑148 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGTCTTTTGCGCCTGAata > 2:347851/1‑149 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGCCCGCTCGCGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTTCGCCTGAatat > 5:15441/1‑149 (MQ=255) gTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGGGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGga < 3:117430/149‑1 (MQ=255) gTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGga < 5:257763/149‑1 (MQ=255) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:155875/149‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg > 1:76992/1‑148 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa > 2:217385/1‑149 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 1:71325/149‑1 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTATCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc > 4:385442/1‑148 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCGCTCCCTCAAGGGAGAGGGGCCCGATCGAGCACAAATTTTGAGAGCTATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCGGAATATTGCGGAGAAAAAGCa > 8:326581/1‑149 (MQ=255) tCAGCGCGCACGTTCACCCGCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAACTTCTTCGGTCTTGTAAAAGAAGAAAAG‑AAAATACGGCTATTGCGCCTGAATATTGCGGAGAAAAAGCa > 2:71325/1‑149 (MQ=255) aGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGTAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt < 1:303047/148‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgc < 8:142423/149‑1 (MQ=255) caccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg > 5:292722/1‑149 (MQ=255) accctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa < 1:21882/147‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAatat > 1:274714/1‑111 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt > 2:314692/1‑127 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc > 2:142772/1‑148 (MQ=255) gtccccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGga < 8:121288/112‑1 (MQ=255) gtccccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGga > 7:121288/4‑115 (MQ=255) gtccccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 5:270322/118‑1 (MQ=255) gtccccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa > 6:270322/4‑121 (MQ=255) gtccccTCTCACTTGAGGGAGAGGGGACCGATAGAGCACAAATTTTGAGAGATATCTTATTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAt > 6:50293/4‑149 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 6:15441/148‑1 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTg < 6:292722/149‑1 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTg < 6:134811/149‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAata < 6:309037/105‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAata > 5:309037/1‑105 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGc > 3:139244/1‑109 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGc < 4:139244/109‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGTAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTg > 6:66300/1‑148 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa > 4:116114/1‑149 (MQ=255) ctctCCCTCAAGGGAGAGTGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGAc < 8:116350/149‑1 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 3:178917/1‑149 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 8:419316/112‑1 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa > 7:419316/1‑112 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGCCCGCGAGCCAATTGGACGc > 6:361775/1‑148 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg < 2:82795/147‑1 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCa > 4:150440/1‑147 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCa > 6:315929/1‑147 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCa > 1:229252/1‑147 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt > 5:303790/1‑114 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt < 6:303790/114‑1 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 6:159524/1‑149 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 2:237228/1‑149 (MQ=255) cAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGTTATCTTCTTCTGTCTTGTAACAGTAGAACAGAAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg < 2:27534/149‑1 (MQ=255) | CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGG > NC_000913/164472‑164767 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |