Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,011,082 C→A 5.7% S373R (AGC→AGA)  uup → replication regulatory ABC‑F family DNA‑binding ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,011,0820CA 5.7% 222.3 / 2.6 70S373R (AGC→AGA) uupreplication regulatory ABC‑F family DNA‑binding ATPase
Reads supporting (aligned to +/- strand):  ref base C (32/34);  new base A (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

CGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAGCCACGCCACGTATTGG  >  NC_000913/1010935‑1011227
                                                                                                                                                   |                                                                                                                                                 
cGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCg                                                                                                                                                  <  2:64700/149‑1 (MQ=255)
  tttGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCggg                                                                                                                                                <  4:293013/149‑1 (MQ=255)
       tACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTAt                                                                                                                                           >  6:10344/1‑149 (MQ=255)
        aCCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTAtt                                                                                                                                          >  1:67858/1‑149 (MQ=255)
         ccAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGGGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTAtt                                                                                                                                          <  2:32232/148‑1 (MQ=255)
          cAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCa                                                                                                                                        >  5:254597/1‑149 (MQ=255)
          cAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCa                                                                                                                                        >  8:234216/1‑149 (MQ=255)
              ttAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGtt                                                                                                                                    <  2:112107/149‑1 (MQ=255)
                aaCGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTg                                                                                                                                   <  8:374696/148‑1 (MQ=255)
                aaCGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTg                                                                                                                                   <  6:254597/148‑1 (MQ=255)
                 aCGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTgg                                                                                                                                  <  8:426613/148‑1 (MQ=255)
                       aGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCaaa                                                                                                                           <  6:141343/149‑1 (MQ=255)
                        gCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCg                                                                                                                                                  <  1:383758/125‑1 (MQ=255)
                        gCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCg                                                                                                                                                  >  2:383758/1‑125 (MQ=255)
                         cAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGtt                                                                                                                                    >  7:343818/1‑138 (MQ=255)
                         cAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGtt                                                                                                                                    <  8:343818/138‑1 (MQ=255)
                            cTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGAATGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGCTGCTC‑GTCAGCATACAGCCGACAGCCCGCCTATTAACGGTGGCACCACACTTGa                                                                                                                      >  4:258055/1‑148 (MQ=255)
                               gTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAg                                                                                                                    <  1:322746/148‑1 (MQ=255)
                                 gAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTgg                                                                                                                 >  8:141639/1‑149 (MQ=255)
                                  aaaGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGc                                                                                                                >  1:49907/1‑149 (MQ=255)
                                  aaaGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGc                                                                                                                >  5:104029/1‑149 (MQ=255)
                                     gATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGCCGGCGGGCGTATTCCCGTTGGCACCAAACTGGAAGTGGCTTa                                                                                                             >  5:282591/1‑149 (MQ=255)
                                      aTTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTAt                                                                                                            >  1:220617/1‑149 (MQ=255)
                                           tCTGCCCAGGTGCTACGTGGCGACAAAATTGCCCTGAGTGGTCCGAAGGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCg                                                                                                        <  5:119056/148‑1 (MQ=255)
                                               cccAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGAGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAg                                                                                                   >  6:64900/1‑149 (MQ=255)
                                               cccAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGAGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAg                                                                                                   >  6:58977/1‑149 (MQ=255)
                                                ccAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAg                                                                                                   >  5:174737/1‑148 (MQ=255)
                                                     ttCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACcgc                                                                                             >  1:326375/1‑149 (MQ=255)
                                                        tACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAACACCCCGCTGCTAAAGCTGATGCTCGGTCAGCTTCAAGCGGCCAGCGGGCGTATTCCCGTTGGCACCCAACTGGAAGTGGCTGATTTCGATCAGCCCcgcgcg                                                                                          >  4:65143/1‑149 (MQ=255)
                                                        tACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTAttt                                                                                                          <  6:403425/133‑1 (MQ=255)
                                                        tACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTAttt                                                                                                          >  5:403425/1‑133 (MQ=255)
                                                           gTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGaa                                                                                       <  6:174737/149‑1 (MQ=255)
                                                            tGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACcgcgcg                                                                                          <  6:295394/145‑1 (MQ=255)
                                                            tGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACcgcgcg                                                                                          >  5:295394/1‑145 (MQ=255)
                                                             ggCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCggg                                                                                                                                                >  8:418217/1‑90 (MQ=255)
                                                             ggCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCggg                                                                                                                                                <  7:418217/90‑1 (MQ=255)
                                                                           ccTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATaaaa                                                                       >  1:212875/1‑149 (MQ=255)
                                                                           ccTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATaaaa                                                                       <  7:234216/149‑1 (MQ=255)
                                                                                  ggTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGa                                                                 <  2:67858/148‑1 (MQ=255)
                                                                                  ggTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGa                                                                 <  2:326375/148‑1 (MQ=255)
                                                                                   gTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATg                                                               >  1:48631/1‑149 (MQ=255)
                                                                                   gTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATg                                                               <  5:10344/149‑1 (MQ=255)
                                                                                    tCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATg                                                               >  8:118741/1‑148 (MQ=255)
                                                                                     ccGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGa                                                             <  6:104029/149‑1 (MQ=255)
                                                                                            ggTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCtt                                                      <  7:141639/149‑1 (MQ=255)
                                                                                              tGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGAGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGc                                                    <  5:58977/149‑1 (MQ=255)
                                                                                              tGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGAGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGc                                                    <  5:64900/149‑1 (MQ=255)
                                                                                                 ggCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCg                                                  >  3:335666/1‑148 (MQ=255)
                                                                                                  gCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGaa                                                <  7:32437/149‑1 (MQ=255)
                                                                                                  gCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGAGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCg                                                  >  4:232683/1‑147 (MQ=255)
                                                                                                   cAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGTGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAg                                               <  1:104339/149‑1 (MQ=255)
                                                                                                     aaaCCACGCTGCTAAAACTGATGCTCGGTCAGCTTCCAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGAATCCCGATAAAACGGTGATGGATAACCTTGCCGAAgg                                              >  1:224745/1‑148 (MQ=255)
                                                                                                     aaaCCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGaa                                                                                       >  7:332804/1‑107 (MQ=255)
                                                                                                     aaaCCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGaa                                                                                       <  8:332804/107‑1 (MQ=255)
                                                                                                       aCCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGt                                             >  4:237395/1‑147 (MQ=255)
                                                                                                         cACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAg                                          >  3:165797/1‑148 (MQ=255)
                                                                                                         cACGCTGCTAAAACTGATGCTCGGTCAGCTGCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTAATTCGATCAGCAACGCCCGGAACTGGATCCCGATAAAACGGAGATGGATAACCTTGCCGAAGCTAAg                                          >  6:179945/1‑148 (MQ=255)
                                                                                                                   aaaCTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGAt                               <  7:118741/149‑1 (MQ=255)
                                                                                                                    aaCTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATg                              >  2:54212/1‑149 (MQ=255)
                                                                                                                     aCTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATgg                             >  4:224931/1‑149 (MQ=255)
                                                                                                                         aTGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCTTAACTGGATCCCGTTAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTaa                         <  7:86040/149‑1 (MQ=255)
                                                                                                                               ggTCAGCTTCAAGCGGACAGCGGGCGTATGCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCaa                   <  3:65143/149‑1 (MQ=255)
                                                                                                                                    gCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAgccac              <  2:48631/149‑1 (MQ=255)
                                                                                                                                     cTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAgccacg             >  8:414179/1‑149 (MQ=255)
                                                                                                                                        cAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAgccacgcc           <  4:335666/148‑1 (MQ=255)
                                                                                                                                           gCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAGccacgccacg        >  2:352149/1‑148 (MQ=255)
                                                                                                                                             ggACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAGCCACGCCACGTAt     <  2:220617/149‑1 (MQ=255)
                                                                                                                                              gACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGCAGGTAAGCAAGAGGTGATGGTTAACGGCAAGCCACGCCACGTAtt    <  2:49907/149‑1 (MQ=255)
                                                                                                                                              gACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGAATCCCGATAAAACGGTGATGTATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAGCCACGCCACGTAtt    <  2:224745/149‑1 (MQ=255)
                                                                                                                                                cAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAGCCACGCCACGTATTgg  <  3:224931/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGGCAAAACCACGCTGCTAAAACTGATGCTCGGTCAGCTTCAAGCGGACAGCGGGCGTATTCACGTTGGCACCAAACTGGAAGTGGCTTATTTCGATCAGCACCGCGCGGAACTGGATCCCGATAAAACGGTGATGGATAACCTTGCCGAAGGTAAGCAAGAGGTGATGGTTAACGGCAAGCCACGCCACGTATTGG  >  NC_000913/1010935‑1011227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑