Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,176,906 C→T 6.6% H96H (CAC→CAT lolD → outer membrane‑specific lipoprotein transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,176,9060CT 6.6% 186.5 / 2.6 60H96H (CAC→CATlolDouter membrane‑specific lipoprotein transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (27/29);  new base T (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

GTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAAC  >  NC_000913/1176758‑1177054
                                                                                                                                                    |                                                                                                                                                    
gTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCGACCAGAAGCTGGGCTTTATTTATCAGTTTcac                                                                                                                                                      >  1:163561/1‑149 (MQ=255)
gTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGGGATTTTTAATGGGCCGCCAATGAGCAAACTGGCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTcac                                                                                                                                                      <  5:291401/149‑1 (MQ=255)
 tAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCACCCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTcac                                                                                                                                                      >  7:377611/1‑148 (MQ=255)
          cTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCtgctgc                                                                                                                                            >  6:12557/1‑149 (MQ=255)
          cTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCtgctgc                                                                                                                                            >  2:414980/1‑149 (MQ=255)
             gctgcACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGGCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGcc                                                                                                                                           <  5:155665/147‑1 (MQ=255)
                 cACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGAttt                                                                                                                                     <  4:167909/149‑1 (MQ=255)
                   ccTGCTGGGCGGGCTGGATACGCCAAGCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTa                                                                                                                                   <  3:302756/149‑1 (MQ=255)
                    ctgctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTAc                                                                                                                                  <  5:15979/149‑1 (MQ=255)
                     tgctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTa                                                                                                                                   <  8:159638/147‑1 (MQ=255)
                     tgctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTa                                                                                                                                   >  7:159638/1‑147 (MQ=255)
                       ctgGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGc                                                                                                                               <  3:30035/149‑1 (MQ=255)
                           gtgggcTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTg                                                                                                                           <  8:308249/147‑1 (MQ=255)
                                    aTACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAGCCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTg                                                                                                                  <  5:73054/149‑1 (MQ=255)
                                      aCGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGc                                                                                                                >  8:90246/1‑149 (MQ=255)
                                       cGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGc                                                                                                                <  1:285874/148‑1 (MQ=255)
                                          cAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGACCTGCGCCACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATg                                                                                                            >  8:413091/1‑149 (MQ=255)
                                              cTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTAcc                                                                                                                                 >  6:185372/1‑124 (MQ=255)
                                              cTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTAcc                                                                                                                                 <  5:185372/124‑1 (MQ=255)
                                              cTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCgc                                                                                                        >  2:18400/1‑149 (MQ=255)
                                               tCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCgct                                                                                                       >  7:325377/1‑149 (MQ=255)
                                                   gCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCgctgct                                                                                                    >  1:277/1‑148 (MQ=255)
                                                      aTGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCGACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGAtt                                                                                                <  2:163561/149‑1 (MQ=255)
                                                      aTGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGAtt                                                                                                <  5:12557/149‑1 (MQ=255)
                                                           aTTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCaa                                                                                           <  4:333824/149‑1 (MQ=255)
                                                            tttttAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCATCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCgct                                                                                                       >  2:299090/1‑136 (MQ=255)
                                                            tttttAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCATCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCgct                                                                                                       >  2:293351/1‑136 (MQ=255)
                                                            tttttAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCATCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCgct                                                                                                       <  1:293351/136‑1 (MQ=255)
                                                            tttttAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCATCACCTGCTGCCGGATTTTACCGACCTGGAAAACGTGGCTATGCCgct                                                                                                       <  1:299090/136‑1 (MQ=255)
                                                                 aaTGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACctgc                                                                                                                                               <  3:63065/91‑1 (MQ=255)
                                                                 aaTGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACctgc                                                                                                                                               >  4:63065/1‑91 (MQ=255)
                                                                      tCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCg                                                                                <  2:125634/149‑1 (MQ=255)
                                                                            aaTGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGccc                                                                                                                             <  5:340946/98‑1 (MQ=255)
                                                                            aaTGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGaaa                                                                          <  8:377611/149‑1 (MQ=255)
                                                                            aaTGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGaaa                                                                          <  5:82215/149‑1 (MQ=255)
                                                                            aaTGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCAGCCGGATTTTACCGccc                                                                                                                             >  6:340946/1‑98 (MQ=255)
                                                                              tGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAAt                                                                         >  6:99061/1‑148 (MQ=255)
                                                                              tGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATc                                                                        <  1:18400/149‑1 (MQ=255)
                                                                                         tcgtcgGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGc                                                             <  1:414980/149‑1 (MQ=255)
                                                                                           gtcgGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCCCCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCAc                                                           >  5:222964/1‑149 (MQ=255)
                                                                                           gtcgGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTAc                                                                                                                                  >  8:233770/1‑78 (MQ=255)
                                                                                           gtcgGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTAc                                                                                                                                  <  7:233770/78‑1 (MQ=255)
                                                                                               gCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTga                                                       <  4:361322/149‑1 (MQ=255)
                                                                                                 aGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTgaga                                                     <  5:99061/149‑1 (MQ=255)
                                                                                                  gCGAAAGCTGAACTGCGCAACCAGACGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGAt                                                    >  6:379999/1‑149 (MQ=255)
                                                                                                  gCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCATGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGAt                                                    >  5:22840/1‑149 (MQ=255)
                                                                                                   cGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGAt                                                                                                 <  4:165924/103‑1 (MQ=255)
                                                                                                   cGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGAt                                                                                                 >  3:165924/1‑103 (MQ=255)
                                                                                                               tGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCCCCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTg                                       >  2:20235/1‑149 (MQ=255)
                                                                                                                 cgcAAACAGAAGGTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTggg                                     <  2:258330/149‑1 (MQ=255)
                                                                                                                    aaCCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCt                                  <  7:90246/149‑1 (MQ=255)
                                                                                                                      ccAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTg                                 >  3:217384/1‑148 (MQ=255)
                                                                                                                       cAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGa                               >  5:154231/1‑149 (MQ=255)
                                                                                                                         gAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGAt                                                                                                 <  1:186608/81‑1 (MQ=255)
                                                                                                                         gAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGAt                                                                                                 >  2:186608/1‑81 (MQ=255)
                                                                                                                         gAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGat                              >  8:366409/1‑148 (MQ=255)
                                                                                                                                  cTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGCAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCg                     >  3:254749/1‑148 (MQ=255)
                                                                                                                                  cTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCg                     >  1:258402/1‑148 (MQ=255)
                                                                                                                                       tttatCAGTTTCACCACCTGCTGCCGGATTTTACGGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCa                <  8:325377/148‑1 (MQ=255)
                                                                                                                                          atCAGTTTCACCACCTGCTGCCTGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCg             <  7:413091/148‑1 (MQ=255)
                                                                                                                                          atCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAACCGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGc            >  2:412232/1‑149 (MQ=255)
                                                                                                                                          atCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGc            >  7:165589/1‑149 (MQ=255)
                                                                                                                                               tttCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATc       >  5:327275/1‑149 (MQ=255)
                                                                                                                                                    ccaccTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTgg                                <  7:82326/119‑1 (MQ=255)
                                                                                                                                                    ccaccTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTgg                                >  8:82326/1‑119 (MQ=255)
                                                                                                                                                    ccaccTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAAc  >  3:65771/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTCACCACCTGCTGCCGGATTTTACCGCCCTGGAAAACGTGGCTATGCCGCTGCTGATTGGCAAGAAAAAGCCCGCTGAAATCAACAGCCGTGCACTTGAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAAC  >  NC_000913/1176758‑1177054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: