Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,672 C→T 100% intergenic (‑50/+110) tyrT ← / ← purU tRNA‑Tyr/formyltetrahydrofolate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,6720CT97.9% 322.5 / ‑2.1 95intergenic (‑50/+110)tyrT/purUtRNA‑Tyr/formyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (51/42);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGAC  >  NC_000913/1287528‑1287817
                                                                                                                                                |                                                                                                                                                 
tggtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGtatc                                                                                                                                                <  7:153032/148‑1 (MQ=255)
  gtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCata                                                                                                                                             >  8:54758/1‑149 (MQ=255)
  gtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCata                                                                                                                                             >  8:228273/1‑149 (MQ=255)
  gtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCata                                                                                                                                             >  3:313503/1‑149 (MQ=255)
  gtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCata                                                                                                                                             >  1:221477/1‑149 (MQ=255)
   tgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCatat                                                                                                                                            >  3:73126/1‑149 (MQ=255)
   tgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCatat                                                                                                                                            >  2:224793/1‑149 (MQ=255)
   tgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCatat                                                                                                                                            <  6:356182/149‑1 (MQ=255)
          tggtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGa                                                                                                                                     <  5:118587/149‑1 (MQ=255)
                 gggggAAGGATTCGAACATTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGTAACCCCACCACGGGGTAATGCTTTTTACAGGCCTGCTCACTTATAGCGAAGCTGGGCGTATCATAGGAATTTACGctccg                                                                                                                              >  6:383808/1‑149 (MQ=17)
                  ggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGcgccgc                                                                                                                             >  6:14115/1‑149 (MQ=255)
                     gAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTg                                                                                                                           >  2:170725/1‑148 (MQ=255)
                     gAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTg                                                                                                                           >  4:81162/1‑148 (MQ=255)
                        ggATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTa                                                                                                                         >  7:143426/1‑147 (MQ=255)
                              gAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTa                                                                                                                 >  2:61667/1‑149 (MQ=255)
                              gAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTa                                                                                                                 >  1:22417/1‑149 (MQ=255)
                               aaCCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTAc                                                                                                                <  7:228273/149‑1 (MQ=255)
                                aCCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACg                                                                                                               >  6:27817/1‑149 (MQ=255)
                                  cTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGtt                                                                                                             >  7:231425/1‑149 (MQ=255)
                                  cTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGtt                                                                                                             >  2:300714/1‑149 (MQ=255)
                                  cTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGtt                                                                                                             >  3:101656/1‑149 (MQ=255)
                                   ttCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGtt                                                                                                             >  7:169581/1‑148 (MQ=255)
                                            gATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAAt                                                                                                    >  7:377352/1‑148 (MQ=255)
                                            gATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATg                                                                                                   <  3:81162/149‑1 (MQ=255)
                                              tGACGGGAGATTTTGAGTCTGGTGACTTTGGCAGCTCGGGAACCCAACAAAGTGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGTGAAGCGGGGCGTATCTTATCAAATGACGCGCCGATGTAAAGTGTTACGTTGAGAAAAATGaa                                                                                                 <  8:143426/149‑1 (MQ=255)
                                                aCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGTGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAAc                                                                                                <  1:224793/148‑1 (MQ=255)
                                                aCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAAc                                                                                                <  4:313503/148‑1 (MQ=255)
                                                aCGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAAc                                                                                                <  2:221477/148‑1 (MQ=255)
                                                  ggCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTgg                                                                                             >  1:340885/1‑149 (MQ=255)
                                                  ggCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTgg                                                                                             <  1:26035/149‑1 (MQ=255)
                                                      gATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGc                                                                                         >  1:14100/1‑149 (MQ=255)
                                                       aTTTACAGTCTGCTCCCTTTGGGCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCTAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGc                                                                                         <  5:383808/148‑1 (MQ=255)
                                                         ttACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGt                                                                                       >  3:267245/1‑148 (MQ=255)
                                                               tCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAAtttt                                                                                 >  7:332794/1‑148 (MQ=255)
                                                                 tGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCAt                                                                              >  2:107363/1‑149 (MQ=255)
                                                                 tGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCAt                                                                              >  4:91510/1‑149 (MQ=255)
                                                                  gCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATc                                                                             >  8:75322/1‑149 (MQ=255)
                                                                    tCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGaa                                                                                                 <  3:105011/127‑1 (MQ=255)
                                                                    tCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGaa                                                                                                 >  4:105011/1‑127 (MQ=255)
                                                                      ccTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCg                                                                           <  1:300714/147‑1 (MQ=255)
                                                                       cTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCAGTaa                                                                        >  7:282943/1‑149 (MQ=255)
                                                                          tGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCGCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACg                                                                      <  5:27817/148‑1 (MQ=255)
                                                                          tGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAAc                                                                       <  2:379202/147‑1 (MQ=255)
                                                                              cGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGAtt                                                                  <  2:214642/148‑1 (MQ=255)
                                                                              cGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGAtt                                                                  >  1:214642/1‑148 (MQ=255)
                                                                               gCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTa                                                                 >  8:252166/1‑148 (MQ=255)
                                                                               gCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTa                                                                 >  8:46110/1‑148 (MQ=255)
                                                                               gCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTa                                                                 >  6:151298/1‑148 (MQ=255)
                                                                                 tCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAg                                                              >  1:9223/1‑149 (MQ=255)
                                                                                   gggAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAgg                                                             >  8:287316/1‑148 (MQ=255)
                                                                                   gggAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGt                                                            <  2:326533/149‑1 (MQ=255)
                                                                                     gAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTaa                                                          <  5:61897/149‑1 (MQ=255)
                                                                                         cccACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAg                                                      >  1:142738/1‑149 (MQ=255)
                                                                                           caccacGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTa                                                                                                                 <  1:331364/88‑1 (MQ=255)
                                                                                           caccacGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTa                                                                                                                 >  2:331364/1‑88 (MQ=255)
                                                                                           caccacGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGt                                                     >  1:149423/1‑148 (MQ=255)
                                                                                             ccacGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGGATCGGATTAAAGGTAACCAGTTAt                                                  >  1:291590/1‑149 (MQ=255)
                                                                                              cacGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAtt                                                 <  7:5834/149‑1 (MQ=255)
                                                                                                 ggggTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAttttt                                              <  6:305483/149‑1 (MQ=255)
                                                                                                 ggggTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATAATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAttttt                                              <  1:25850/149‑1 (MQ=255)
                                                                                                   ggTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGc                                            >  1:48714/1‑149 (MQ=255)
                                                                                                    gTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCt                                           >  2:359937/1‑149 (MQ=255)
                                                                                                        tGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGc                                        >  8:198259/1‑148 (MQ=255)
                                                                                                           tttttACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGAt                                     >  8:318833/1‑148 (MQ=255)
                                                                                                               tACTGGCCTGCTCCCTTATCGGGAAGCGGTGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTaaa                                 <  2:149423/148‑1 (MQ=255)
                                                                                                               tACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTaaa                                 <  1:170725/148‑1 (MQ=255)
                                                                                                               tACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTaaa                                 <  7:252166/148‑1 (MQ=255)
                                                                                                               tACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTaaa                                 <  6:121100/148‑1 (MQ=255)
                                                                                                                 cTGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGaa                              <  2:22417/149‑1 (MQ=255)
                                                                                                                  tGGCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGaa                              >  1:409378/1‑148 (MQ=255)
                                                                                                                    gCCTGCTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGaataa                           >  6:4065/1‑149 (MQ=255)
                                                                                                                         cTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGtt                      <  4:73126/149‑1 (MQ=255)
                                                                                                                         cTCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGt                       <  7:54758/148‑1 (MQ=255)
                                                                                                                         cTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGtt                      <  2:48714/149‑1 (MQ=255)
                                                                                                                          tCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAttt                                                <  8:75590/122‑1 (MQ=255)
                                                                                                                          tCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTAttt                                                >  7:75590/1‑122 (MQ=255)
                                                                                                                          tCCCTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTc                     >  6:253963/1‑149 (MQ=255)
                                                                                                                            ccTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGa                   >  6:393738/1‑149 (MQ=255)
                                                                                                                            ccTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGa                   <  1:107363/149‑1 (MQ=255)
                                                                                                                            ccTTATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATAAAGAATAATCGTTCGa                   <  3:91510/148‑1 (MQ=255)
                                                                                                                              ttATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGAt                  >  1:330180/1‑148 (MQ=255)
                                                                                                                              ttATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGAt                  >  4:368991/1‑148 (MQ=255)
                                                                                                                              ttATCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGAt                  >  8:130358/1‑148 (MQ=255)
                                                                                                                                 tCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTg                                          <  7:391244/121‑1 (MQ=255)
                                                                                                                                 tCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTg                                          >  8:391244/1‑121 (MQ=255)
                                                                                                                                 tCGGGAAGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTAc               <  7:318833/148‑1 (MQ=255)
                                                                                                                                     gAATCGTGGCGTATCATATCAAATGACGCGACTATGTAAAGTGTTACTTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTAcc              <  4:101656/145‑1 (MQ=255)
                                                                                                                                      aaGCGGTGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa         <  5:274057/149‑1 (MQ=255)
                                                                                                                                      aaGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa         <  5:393738/149‑1 (MQ=255)
                                                                                                                                      aaGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa         <  5:4065/149‑1 (MQ=255)
                                                                                                                                      aaGCGGGGCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTaaa         <  2:340885/149‑1 (MQ=255)
                                                                                                                                          ggggCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACaaa     <  5:151298/149‑1 (MQ=255)
                                                                                                                                          ggggCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACaaa     <  3:368991/149‑1 (MQ=255)
                                                                                                                                          ggggCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACaaa     <  2:9223/149‑1 (MQ=255)
                                                                                                                                            ggCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGa   >  5:36481/1‑149 (MQ=255)
                                                                                                                                            ggCGTATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGa   <  7:198259/149‑1 (MQ=255)
                                                                                                                                             gcgtATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGAc  <  8:169581/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGAC  >  NC_000913/1287528‑1287817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: