Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 217,593 G→T 5.1% P395T (CCA→ACA)  proS ← prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913217,5930GT 5.1% 274.3 / 3.5 79P395T (CCA→ACA) proSprolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (40/35);  new base T (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

CAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACC  >  NC_000913/217448‑217738
                                                                                                                                                 |                                                                                                                                                 
cAACCCCTCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATc                                                                                                                                                <  5:45238/149‑1 (MQ=255)
 aaCCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCg                                                                                                                                               >  4:330888/1‑149 (MQ=255)
   cccATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCgc                                                                                                                                              >  4:326025/1‑148 (MQ=255)
      aTCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTGCTATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCgccagc                                                                                                                                          >  5:267423/1‑149 (MQ=255)
                  tGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTAc                                                                                                                               <  1:28719/148‑1 (MQ=255)
                    gTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGa                                                                                                                            <  1:7454/149‑1 (MQ=255)
                    gTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGa                                                                                                                            <  6:373792/149‑1 (MQ=255)
                       aCGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGata                                                                                                                          <  7:11950/148‑1 (MQ=255)
                         ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACg                                                                                                                              <  8:256426/142‑1 (MQ=255)
                         ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCt                                                                                                                       <  5:253697/149‑1 (MQ=255)
                         ggCCATCTTCACCCTGTACGGAGCCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACg                                                                                                                              >  7:256426/1‑142 (MQ=255)
                              tCTTCACACTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGACTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAAc                                                                                                                  >  8:255390/1‑149 (MQ=255)
                                 tCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTc                                                                                                               >  1:245477/1‑149 (MQ=255)
                                    cccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCgg                                                                                                            >  7:268876/1‑149 (MQ=255)
                                     ccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTCCCggg                                                                                                           >  3:210298/1‑149 (MQ=255)
                                         tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTa                                                                                                        <  7:403468/148‑1 (MQ=255)
                                            ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                                                    <  3:330888/149‑1 (MQ=255)
                                                      aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATGCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc                                                                                          >  2:100816/1‑149 (MQ=255)
                                                      aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAt                                                                                           <  8:46210/148‑1 (MQ=255)
                                                      aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc                                                                                          <  3:326025/149‑1 (MQ=255)
                                                               gAGTACTTGTTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                 <  2:245477/149‑1 (MQ=255)
                                                               gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGTATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                 >  7:139581/1‑149 (MQ=255)
                                                               gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGTATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                 >  7:133420/1‑149 (MQ=255)
                                                                 gTACTTGGTACGCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATAAGCAGCCTACCTTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                               <  4:6151/149‑1 (MQ=255)
                                                                     ttGGTTCCCAGCTGGAAGATGTGACCAACTTCCATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                           <  6:267423/149‑1 (MQ=255)
                                                                     ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAg                                                                                      <  1:422068/138‑1 (MQ=255)
                                                                     ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAg                                                                                      >  2:422068/1‑138 (MQ=255)
                                                                       ggTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGTATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                           <  8:133420/147‑1 (MQ=255)
                                                                       ggTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGTATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                           <  8:139581/147‑1 (MQ=255)
                                                                       ggTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAag                                                                          >  6:263623/1‑148 (MQ=255)
                                                                        gTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTGGATGCCGAag                                                                          >  3:281977/1‑147 (MQ=255)
                                                                           cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTg                                                                                                                      <  6:388710/100‑1 (MQ=255)
                                                                           cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTg                                                                                                                      >  5:388710/1‑100 (MQ=255)
                                                                            ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtgt                                                                    >  5:388706/1‑149 (MQ=255)
                                                                            ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                     >  1:151113/1‑148 (MQ=255)
                                                                            ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                     <  2:151113/148‑1 (MQ=255)
                                                                              aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                 >  3:193865/1‑134 (MQ=255)
                                                                              aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                 <  4:193865/134‑1 (MQ=255)
                                                                                    aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCgg                                                                                                            <  5:102815/101‑1 (MQ=255)
                                                                                    aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCgg                                                                                                            >  6:102815/1‑101 (MQ=255)
                                                                                    aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                            <  1:314673/149‑1 (MQ=255)
                                                                                    aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                            <  8:268876/149‑1 (MQ=255)
                                                                                          tgACCACCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGCTCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                                                      <  6:305381/149‑1 (MQ=255)
                                                                                           gACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                       >  3:192803/1‑147 (MQ=255)
                                                                                              cAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGTGTAGCGACATCGCGAGCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCa                                                  <  2:69788/149‑1 (MQ=255)
                                                                                               aaCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAc                                                 >  8:212279/1‑149 (MQ=255)
                                                                                                   tCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagt                                                                         <  1:13064/121‑1 (MQ=255)
                                                                                                   tCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagt                                                                         >  2:13064/1‑121 (MQ=255)
                                                                                                          cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaa                                       <  5:64234/148‑1 (MQ=255)
                                                                                                          cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaa                                       >  6:64234/1‑148 (MQ=255)
                                                                                                          cACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCACAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCCGCAGCGaaa                                      >  6:301290/1‑149 (MQ=255)
                                                                                                          cACGTTTGATCAGCAGCCTACCCTGGCCATCAGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGCCATAGCGATACCAGTTGATGCCGAAGTAGTGGTTAACATCGATGTTAGCACCAGCAGCGaa                                       >  4:279051/1‑148 (MQ=255)
                                                                                                          cACGTTTGATCAGCAGCCTAACCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                      >  1:303307/1‑149 (MQ=255)
                                                                                                          cACGTTTGATCAGAAGCATACCCTTGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTCCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                      <  4:210298/149‑1 (MQ=255)
                                                                                                             gTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                                                      >  1:246492/1‑130 (MQ=255)
                                                                                                             gTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                                                      <  2:246492/130‑1 (MQ=255)
                                                                                                                 gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCTAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAATTCACTCa                               >  3:2388/1‑149 (MQ=255)
                                                                                                                    cagcagCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcg                            >  3:254273/1‑149 (MQ=255)
                                                                                                                     agcagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgc                           >  3:38712/1‑149 (MQ=255)
                                                                                                                     agcagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgc                           >  3:359009/1‑149 (MQ=255)
                                                                                                                      gcagcCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACAACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCCCTCATcgcc                          >  6:264739/1‑149 (MQ=255)
                                                                                                                            tACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTCGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacg                    >  2:220025/1‑149 (MQ=255)
                                                                                                                             aCCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCACCTTCCGGGGTAGCGACATCGCGCTCCCAGTTGATGCCGAAGTAGTGTTTACATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggt                  >  1:343531/1‑149 (MQ=255)
                                                                                                                             aCCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                   >  6:110726/1‑69 (MQ=255)
                                                                                                                             aCCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTACCGGGGTAGCGACATCGCGATCCCAGATGATGCCGAAGAAGTCGTTACCATCGATGTTACCACCAGCAGCGAAATCACTCAACGCCGCAacgg                   >  7:429228/1‑149 (MQ=255)
                                                                                                                              cccTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacgg                   <  7:255390/148‑1 (MQ=255)
                                                                                                                               ccTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggta                 <  6:388706/149‑1 (MQ=255)
                                                                                                                                cTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                        >  1:266317/1‑93 (MQ=255)
                                                                                                                                cTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                        <  2:266317/93‑1 (MQ=255)
                                                                                                                                 tGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                            >  4:47735/1‑72 (MQ=255)
                                                                                                                                 tGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                            <  3:47735/72‑1 (MQ=255)
                                                                                                                                     gaTCCGGGCTTGGATCGCCAGCCACCACGTTACTGATATCTGAAACTTCCGGGGTAGCGACATCGCGATGCCAGTTGATGCGGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCa           <  3:279051/148‑1 (MQ=255)
                                                                                                                                       tCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                        >  2:141611/1‑86 (MQ=255)
                                                                                                                                       tCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                        <  1:141611/86‑1 (MQ=255)
                                                                                                                                       tCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt         >  6:355473/1‑149 (MQ=255)
                                                                                                                                        ccGTGCTTGGATCGCCAGCCACCACGTTACGGTTATCTGCAACTTGCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt         <  8:429228/148‑1 (MQ=255)
                                                                                                                                          gggCTTGGATCGCCAGCCACCACGTTACGGATTTCTGCAACTTCCGGGGTAGCGTCTTCGCGCTCCCACTAGATGCCGAACTAGTGTTTACCATCGATGTTAGCCCCCGCAGCGAACTCACTCCGCGCCGCAACGGTCCGGTCACTccc      >  1:294634/1‑147 (MQ=255)
                                                                                                                                             cTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGACAATcaccac   <  4:359009/149‑1 (MQ=255)
                                                                                                                                              ttGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCaccacc  >  2:351096/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACC  >  NC_000913/217448‑217738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: