Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,782,643	A→T	5.3%	N417Y (AAC→TAC)	ydiS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,782,643	0	A	T	5.3%	244.9 / 2.8	76	N417Y (AAC→TAC)	ydiS	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (35/37);  new base T (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GGCGCTGATGGAAAACCCGCGCCTGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATCAAT  >  NC_000913/1782498‑1782788                                                                                                                                                  |                                                                                                                                                  ggCGCTGATGGAAAACCCGCGCCTGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACt                                                                                                                                                >  4:31710/1‑149 (MQ=255)    gcTGATGGAAAACCCGCGCCTGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGc                                                                                                                                             <  4:359412/149‑1 (MQ=255)         ttgAAAACCCGCGCCTGTTTAGCCAATACGCAGGAATGGAAGCCGACATCATGCACGAGATTTTCTCCATCGACGGCATACCAAACCAGCCGGTACGAAAAATGATAATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGaaa                                                                                                                                        <  4:294805/147‑1 (MQ=255)         tGGAAAACCCGCGCCTGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAATGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGAGCTGATCAACTTGCTGaaa                                                                                                                                        <  8:138136/149‑1 (MQ=255)               aCCCGCGCCTGTTTAGCCAATACCCACGAATGGTAGCCGACACCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGc                                                                                                                                  <  2:372691/149‑1 (MQ=255)                     gcCTGTTTAGCCAATACCCACTAATTGTAGACGACATCATGAACGAGATGTTCACCATCGACGGCTAACCTAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAATTTGGGCTGATGAACTTGCTGAAAGATGGCATTAAg                                                                                                                            <  4:339580/149‑1 (MQ=255)                      ccTGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAgg                                                                                                                           <  8:379383/149‑1 (MQ=255)                        tGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAgg                                                                                                                           >  1:360123/1‑147 (MQ=255)                          tttAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGa                                                                                                                         <  8:385815/147‑1 (MQ=255)                          tttAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGa                                                                                                                         <  8:263378/147‑1 (MQ=255)                                  aTACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACGATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCgcgc                                                                                                               <  3:244287/149‑1 (MQ=255)                                  aTACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCgcgc                                                                                                               >  6:348612/1‑149 (MQ=255)                                   tACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCgcgc                                                                                                               >  3:60728/1‑148 (MQ=255)                                     cccACGAATGGTAGCCGCCATCATGAACGAGATGTTCACCATCGACGGCCAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTAt                                                                                                            >  2:366825/1‑149 (MQ=255)                                     cccACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTAt                                                                                                            <  2:360123/149‑1 (MQ=255)                                        aCGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGa                                                                                                                                      <  1:97228/120‑1 (MQ=255)                                        aCGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGa                                                                                                                                      >  2:97228/1‑120 (MQ=255)                                        aCGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCAAGGGACATGCGAAGAAAATTGGTCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAg                                                                                                         >  6:221577/1‑149 (MQ=255)                                               gTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGaa                                                                                                   <  7:114083/148‑1 (MQ=255)                                                tAGCCGCCATCATGAACGAGATGTTCACCATCGACGGCCAACCAAACCAGCCGGTACGAAAAATGATCATGGGAGATGCGAAGAATATTGGGCTGATCAACGTGCGGCAAGCTGGCATTAAGGGAGCAACCGCGCTAGGGGCCAGAACg                                                                                                 >  2:344968/1‑149 (MQ=255)                                                tAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGTCAGAACg                                                                                                 >  6:304517/1‑149 (MQ=255)                                                  gCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTAt                                                                                                            <  7:310176/136‑1 (MQ=255)                                                  gCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTAt                                                                                                            >  8:310176/1‑136 (MQ=255)                                                           atGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAAc                                                                                                                                                 <  8:135194/90‑1 (MQ=255)                                                            tGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACg                                                                                     >  4:126222/1‑149 (MQ=255)                                                             gAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGt                                                                                    >  7:380028/1‑149 (MQ=255)                                                                 gagaTGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGa                                                                                 >  6:320972/1‑148 (MQ=255)                                                                   gaTGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGTCAGAACGCTACGGTTAACGTCGACAt                                                                              <  5:304517/149‑1 (MQ=255)                                                                       ttCACCATCGACGGCAAACCAAACCAGCCGGTACTAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCaa                                                                           >  2:58608/1‑148 (MQ=255)                                                                       ttCAACATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCaa                                                                           <  3:138345/148‑1 (MQ=255)                                                                         cACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAAt                                                                         <  5:348612/148‑1 (MQ=255)                                                                            cATCGACGGCCAACCAAACCAGCCGGTACGAATAATGATCATGCGACATGCGAAGAAAATTGGGCTGATCAACTAGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAgg                                                                     >  5:354627/1‑149 (MQ=255)                                                                             aTCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGc                                                                    >  7:167848/1‑149 (MQ=255)                                                                              tCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCg                                                                   >  7:275239/1‑149 (MQ=255)                                                                                     caaaccaaacCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTc                                                                                   <  2:24350/126‑1 (MQ=255)                                                                                     caaaccaaacCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTc                                                                                   >  1:24350/1‑126 (MQ=255)                                                                                       aaccaaaccaGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAAt                                                          <  3:340573/149‑1 (MQ=255)                                                                                          caaaccaGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAAc                                                                                                                    <  4:145286/88‑1 (MQ=255)                                                                                          caaaccaGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAAc                                                                                                                    >  3:145286/1‑88 (MQ=255)                                                                                          caaaccaGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTc                                                        >  5:393255/1‑148 (MQ=255)                                                                                            aaccaGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCAt                                                     >  8:6655/1‑149 (MQ=255)                                                                                              ccaGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGt                                                   <  3:31710/149‑1 (MQ=255)                                                                                               caGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACAt                                                                              >  1:105809/1‑121 (MQ=255)                                                                                               caGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACAt                                                                              <  2:105809/121‑1 (MQ=255)                                                                                               caGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGtt                                                  <  8:380028/149‑1 (MQ=255)                                                                                                  caggTACTAAAAATGATCATTGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCTCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTATGCGTCAATAAATTCCATGTtgat                                               <  6:343606/147‑1 (MQ=255)                                                                                                    ggTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCTACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTtgat                                               >  2:187398/1‑147 (MQ=255)                                                                                                    ggTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCTACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTtgat                                               >  2:193277/1‑147 (MQ=255)                                                                                                    ggTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTttatga                                             <  1:58608/149‑1 (MQ=255)                                                                                                    ggTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTtgatga                                             <  2:246094/149‑1 (MQ=255)                                                                                                     gTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCg                                                                                  <  4:379451/111‑1 (MQ=255)                                                                                                     gTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCg                                                                                  >  3:379451/1‑111 (MQ=255)                                                                                                     gTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAg                                            >  7:331930/1‑149 (MQ=255)                                                                                                               tGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGc                                  <  5:320972/149‑1 (MQ=255)                                                                                                                      gggACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCAt                           >  7:227620/1‑149 (MQ=255)                                                                                                                             gCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCg                    >  1:64385/1‑149 (MQ=255)                                                                                                                               gaagaaAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCgg                   >  6:178198/1‑148 (MQ=255)                                                                                                                               gaagaaAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGa                  <  1:189430/149‑1 (MQ=255)                                                                                                                                aagaaAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGa                  >  8:322846/1‑148 (MQ=255)                                                                                                                                aagaaAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGa                  >  2:29149/1‑148 (MQ=255)                                                                                                                                aagaaAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGa                  >  3:36145/1‑148 (MQ=255)                                                                                                                                aagaaAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGa                  >  3:329106/1‑148 (MQ=255)                                                                                                                                    aaaTTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATc             <  2:64385/149‑1 (MQ=255)                                                                                                                                     aaTTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATcc            <  7:322846/149‑1 (MQ=255)                                                                                                                                      aTTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATccc           <  8:227620/149‑1 (MQ=255)                                                                                                                                      aTTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATccc           <  4:60728/149‑1 (MQ=255)                                                                                                                                         gggCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGa         <  1:29149/148‑1 (MQ=255)                                                                                                                                            cTGATCTACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATc     <  1:193277/149‑1 (MQ=255)                                                                                                                                            cTGATCTACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATc     <  1:187398/149‑1 (MQ=255)                                                                                                                                            cTGATCAACTTGCTGAAAGATTGCTTTAAGGGAGCAACCGCGCTATGAGCCAGAACTCTACTGTTAAAGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGTCAACGCGCATATAATTCTGGCGGATAATCACGagat      >  2:422952/1‑148 (MQ=255)                                                                                                                                             tGATCAAATTGATGAACTATGTCATTAAGGGAGCAACCGCGCTATGAGCAAGAACGATACGGTTAACGTCGACATCAAATTAGGAGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATc     <  1:422952/148‑1 (MQ=255)                                                                                                                                               aTCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATCAAt  >  6:75764/1‑149 (MQ=255)                                                                                                                                               aTCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATCAAt  >  1:35105/1‑149 (MQ=255)                                                                                                                                               aTCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATCAAt  <  8:275239/149‑1 (MQ=255)                                                                                                                                                tCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTg                       >  6:205442/1‑127 (MQ=255)                                                                                                                                                tCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTg                       <  5:205442/127‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GGCGCTGATGGAAAACCCGCGCCTGTTTAGCCAATACCCACGAATGGTAGCCGACATCATGAACGAGATGTTCACCATCGACGGCAAACCAAACCAGCCGGTACGAAAAATGATCATGGGACATGCGAAGAAAATTGGGCTGATCAACTTGCTGAAAGATGGCATTAAGGGAGCAACCGCGCTATGAGCCAGAACGCTACGGTTAACGTCGACATCAAATTAGGCGTCAATAAATTCCATGTTGATGAGGGCCACCCGCATATCATTCTGGCGGAAAATCCCGATATCAAT  >  NC_000913/1782498‑1782788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑