Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,745,020 T→A 5.2% K107I (AAA→ATA) ‡ trmD ← tRNA m(1)G37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,745,0200TA 5.2% 339.8 / 5.6 108K107I (AAA→ATA) ‡trmDtRNA m(1)G37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (47/55);  new base A (3/3);  total (50/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

AACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCG  >  NC_000913/2744876‑2745167
                                                                                                                                                |                                                                                                                                                   
aaCCGGGAAACGGAGTCAATCAGCTTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCCTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAttttt                                                                                                                                                  <  4:74386/148‑1 (MQ=255)
aaCCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAttttt                                                                                                                                                  <  4:342128/148‑1 (MQ=255)
 aCCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGa                                                                                                                                                <  7:356771/149‑1 (MQ=255)
      gAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAAAATTGATTctg                                                                                                                                           <  1:134325/149‑3 (MQ=255)
                 aaTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGCCCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGCCCGCCCACCAGAATCAATTTTTGATTCGTTGCCAGTTCGc                                                                                                                                >  3:9562/1‑149 (MQ=255)
                 aaTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGtt                                                                                                                                          >  5:338191/1‑139 (MQ=255)
                 aaTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGtt                                                                                                                                          <  6:338191/139‑1 (MQ=255)
                  aTCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCt                                                                                                                               >  4:366397/1‑149 (MQ=255)
                   tCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTg                                                                                                                              <  8:4520/149‑1 (MQ=255)
                     aGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGAc                                                                                                                            <  3:340116/149‑1 (MQ=255)
                          cATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCg                                                                                                                       >  2:87038/1‑149 (MQ=255)
                           aTTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                                                                                                                        <  3:336863/131‑1 (MQ=255)
                           aTTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGc                                                                                                                      <  5:407099/149‑1 (MQ=255)
                            atGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCAATCTTCGTCAATTACGGTTTGCATCACGCGCTCAACTATACCTTCGTACCGACCGCACACCAGAATCAAATTTTGAATCGTTGCCAGTTCGCTAAAGCCCGCt                                                                                                                     >  4:129144/2‑149 (MQ=255)
                                     aCTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGc                                                                                                            <  8:49226/149‑1 (MQ=255)
                                     aCTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGc                                                                                                            <  7:66250/149‑1 (MQ=255)
                                       tCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGa                                                                                                                                                <  4:214590/111‑1 (MQ=255)
                                       tCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGa                                                                                                                                                >  3:214590/1‑111 (MQ=255)
                                       tCACCACCACTGAGAACGTAATAGCCGATTGCCCATTCTTCGTCAATTTCGGATTGGATCACCCGCACATAAATACCTTCGTAGCGACCGCACACCAGAATCAAATTTAGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt                                                                                                          >  2:425516/1‑149 (MQ=255)
                                         accaccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTGGGATCACGCGCTCAGCTATACCTTCGTCGCGACCGCACACCAGAAGCACTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt                                                                                                          <  6:373277/147‑1 (MQ=255)
                                           caccacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGt                                                                                                      >  3:61704/1‑149 (MQ=255)
                                             ccacTGAGAACGTAATCGCCGATTGCCCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTcc                                                                                                    >  5:290499/1‑149 (MQ=255)
                                              cacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGtt                                                                                                                                   >  8:1351/1‑117 (MQ=255)
                                              cacTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGtt                                                                                                                                   <  7:1351/117‑1 (MQ=255)
                                                 tGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCCCGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTGGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATAAAGCTTGCGTCCCtgt                                                                                                >  8:295296/1‑149 (MQ=255)
                                                   agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCTCGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGTTTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgttg                                                                                              <  3:19681/149‑3 (MQ=255)
                                                   agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGc                                                                                                        >  6:36010/1‑139 (MQ=255)
                                                   agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                                                                                               <  7:275247/148‑1 (MQ=255)
                                                   agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                                                                                               <  2:395890/148‑1 (MQ=255)
                                                    gaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                                                                                             <  7:104876/149‑1 (MQ=255)
                                                       cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAc                                                                                          <  3:331219/149‑1 (MQ=255)
                                                       cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAc                                                                                          <  6:290499/149‑1 (MQ=255)
                                                       cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAAAAATTGATTctggt                                                                                                                                         >  3:108530/1‑98 (MQ=255)
                                                       cGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAAGCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGAc                                                                                          <  2:121413/149‑1 (MQ=255)
                                                         tAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAg                                                                                        <  4:61704/149‑1 (MQ=255)
                                                           aTCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGa                                                                                       <  4:248936/148‑1 (MQ=255)
                                                            tCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                                                                                             <  4:304823/141‑1 (MQ=255)
                                                            tCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGAt                                                                                      >  1:379457/1‑148 (MQ=255)
                                                              gCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                                                                                               <  1:268376/137‑1 (MQ=255)
                                                              gCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACAAGAATCAATTTTTGATTAGTTTCCAGTTCGCTGACGCCCGCTTGATCAAGATTGCGTCCCtctg                                                                                               >  2:268376/1‑137 (MQ=255)
                                                                 gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGc                                                                                                        <  5:372392/125‑1 (MQ=255)
                                                                 gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGc                                                                                                        >  6:372392/1‑125 (MQ=255)
                                                                 gATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                <  2:362123/149‑1 (MQ=255)
                                                                      tccATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGGACACGAGAATCAATTTTTGATTCGTTGCCTGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCtt                                                                            <  3:129144/147‑1 (MQ=255)
                                                                         aTTCTTCGTCAATTTCGGTTTGGATCACTCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTTCCAGTTCGATGACGCCCGCTTGATCAAGCTTGCGTCCATGTGGTGACATATAAATCACCTTTgcg                                                                        >  8:106360/1‑149 (MQ=255)
                                                                          ttcttcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgcgc                                                                       <  2:379457/149‑1 (MQ=255)
                                                                            cttcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                                                                                                                        <  7:334435/82‑1 (MQ=255)
                                                                            cttcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGc                                                                                                                                        >  8:334435/1‑82 (MQ=255)
                                                                              tcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGcc                                                                                                           >  7:404182/1‑108 (MQ=255)
                                                                              tcGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGcc                                                                                                           <  8:404182/109‑2 (MQ=255)
                                                                               cGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCt                                                                     <  3:366397/146‑1 (MQ=255)
                                                                                    aTTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACccgc                                                             <  7:260431/149‑1 (MQ=255)
                                                                                     tttCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                  <  2:132389/143‑1 (MQ=255)
                                                                                     tttCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCa                                                                  >  1:132389/1‑143 (MQ=255)
                                                                                     tttCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACccgcc                                                            >  7:55018/1‑149 (MQ=255)
                                                                                           tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCt                                                      >  4:301627/1‑149 (MQ=255)
                                                                                           tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCt                                                      >  3:215533/1‑149 (MQ=255)
                                                                                           tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACACGCCGCGGCt                                                      >  3:111901/1‑149 (MQ=255)
                                                                                              ggATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCttt                                                    <  5:6034/148‑1 (MQ=255)
                                                                                                 tCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                >  5:74519/1‑149 (MQ=255)
                                                                                                    cgcgCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGAGCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCa                                             <  1:87038/149‑1 (MQ=255)
                                                                                                     gcgcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                                                                                             >  4:277794/1‑84 (MQ=255)
                                                                                                     gcgcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                                                                                             <  3:277794/84‑1 (MQ=255)
                                                                                                       gcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGa                                                                                                                  >  7:344288/1‑77 (MQ=255)
                                                                                                       gcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGa                                                                                                                  <  8:344288/77‑1 (MQ=255)
                                                                                                        cTCATCTATACCTTCGTAGCGACCGCACACCAGAAGCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCGTGATCAAGCGTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                         <  7:295296/149‑1 (MQ=255)
                                                                                                         tCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                                                                                                >  1:126736/1‑77 (MQ=255)
                                                                                                         tCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                                                                                                <  2:126736/77‑1 (MQ=255)
                                                                                                           aTCTATACCTTCGTAGCGCCCGCACACCAGAATGAATTTTTGATTCGTTGCCAGGTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                       <  1:425516/148‑1 (MQ=255)
                                                                                                           aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                      >  7:235245/1‑149 (MQ=255)
                                                                                                           aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                       >  5:53214/1‑148 (MQ=255)
                                                                                                           aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                       <  6:53214/148‑1 (MQ=255)
                                                                                                            tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                         >  6:258614/1‑145 (MQ=255)
                                                                                                            tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                         <  5:258614/145‑1 (MQ=255)
                                                                                                             cTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCg                                    >  4:44667/1‑149 (MQ=255)
                                                                                                               ataCCTTCGTAGCGACCGCACCCCAGAATCAATATTTGATTAGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCGTGCGTCCCTGTTGTGACAGAAAAATCACCTTTGCGCCTTCCCCCGCCGCGGCGTTTGCTGCCTGAATGGCGTc                                  >  6:296871/1‑149 (MQ=255)
                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCa                                             >  4:74136/1‑132 (MQ=255)
                                                                                                                     tCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCa                                             <  3:74136/132‑1 (MQ=255)
                                                                                                                       gaaGCGACCTCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAg                           <  5:296871/146‑1 (MQ=255)
                                                                                                                           cGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTg                      <  3:301627/149‑1 (MQ=255)
                                                                                                                            gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc                     <  4:316989/149‑1 (MQ=255)
                                                                                                                             aCCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGCCGCCCGCGTGATCAAg                                                                                                             >  2:260254/1‑60 (MQ=255)
                                                                                                                             aCCGCACACAAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGATTGATCAAg                                                                                                             <  1:260254/60‑1 (MQ=255)
                                                                                                                               cGCACACCAGAATCACTTTTTGCTTCGTTGCCAGTTCGCTGTCGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGCCAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                  <  4:9562/149‑1 (MQ=255)
                                                                                                                                 cacaCCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAATGGTTGCACcat                >  3:134565/1‑149 (MQ=255)
                                                                                                                                  acacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCCCcat                >  6:271794/1‑148 (MQ=255)
                                                                                                                                  acacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatc               <  2:159310/149‑1 (MQ=255)
                                                                                                                                   cacCAGAAGCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatca              <  2:194335/149‑1 (MQ=255)
                                                                                                                                     ccAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatcat             >  7:188139/1‑148 (MQ=255)
                                                                                                                                     ccAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCAtt            <  8:235245/149‑1 (MQ=255)
                                                                                                                                     aacgAATCAAAAATTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtg                                                  >  1:367761/4‑111 (MQ=255)
                                                                                                                                     aacgAATCAAAAATTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtg                                                  <  2:367761/108‑1 (MQ=255)
                                                                                                                                      cAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCAtt            >  6:258102/1‑148 (MQ=255)
                                                                                                                                      cAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCAtt            >  5:307897/1‑148 (MQ=255)
                                                                                                                                       aGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAAt                                        <  7:167573/119‑1 (MQ=255)
                                                                                                                                           tCAATTTTTGATTCGTTGCCAGTTCGCTGGCGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc      >  5:288945/1‑149 (MQ=255)
                                                                                                                                             aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATccc    >  5:174570/1‑149 (MQ=255)
                                                                                                                                             aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATccc    <  3:44667/149‑1 (MQ=255)
                                                                                                                                               tttttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCg  <  4:215533/149‑1 (MQ=255)
                                                                                                                                               tttttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCg  >  5:355560/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCG  >  NC_000913/2744876‑2745167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: