Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 755,400 G→A W75* (TGG→TAG)  sdhC → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,4000GA97.4% 262.5 / ‑2.2 78W75* (TGG→TAG) sdhCsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (42/34);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

CTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTC  >  NC_000913/755261‑755545
                                                                                                                                           |                                                                                                                                                 
ctcCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCt                                                                                                                                          >  2:90479/1‑149 (MQ=255)
   cATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTAc                                                                                                                                       >  1:139312/1‑149 (MQ=255)
     tCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCg                                                                                                                                     >  6:71903/1‑149 (MQ=255)
     tCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCg                                                                                                                                     >  7:77726/1‑149 (MQ=255)
        cgTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGctc                                                                                                                                  >  7:358876/1‑149 (MQ=255)
        cgTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGctc                                                                                                                                  <  3:144339/149‑1 (MQ=255)
             ccGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGc                                                                                                                              <  1:90479/148‑1 (MQ=255)
                 tgtgATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATc                                                                                                                         <  2:329079/149‑1 (MQ=255)
                  gtgCTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCACGCTTCCGGGATTATGGGCAGGTGCTTCGTCATATTTATCATGTAGGGCATCCTTGCCGCTCTGGCGTATCa                                                                                                                        >  4:311373/1‑149 (MQ=255)
                     aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgt                                                                                                                     >  3:164232/1‑149 (MQ=255)
                     aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgt                                                                                                                     >  8:337055/1‑149 (MQ=255)
                     aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgt                                                                                                                     >  1:22786/1‑149 (MQ=255)
                      tCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtc                                                                                                                    <  2:139312/149‑1 (MQ=255)
                         ccTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtc                                                                                                                 <  7:267513/149‑1 (MQ=255)
                         ccTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtc                                                                                                                 <  1:134928/149‑1 (MQ=255)
                          cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  2:261852/1‑149 (MQ=255)
                          cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  6:170048/1‑149 (MQ=255)
                           tttGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                                                                                                >  1:284340/1‑148 (MQ=255)
                              gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTTCCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             <  2:406022/148‑1 (MQ=255)
                              gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             <  8:77726/148‑1 (MQ=255)
                                   aGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTc                                                                                                       <  2:117073/149‑1 (MQ=255)
                                       ggCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCca                                                                                                   >  3:20702/1‑149 (MQ=255)
                                          aTCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGTGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACat                                                                                                >  1:232712/1‑149 (MQ=255)
                                           tCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatg                                                                                               <  4:20702/149‑1 (MQ=255)
                                               gctgTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatga                                                                                           >  1:354119/1‑149 (MQ=255)
                                                ctgTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgat                                                                                          <  7:337055/149‑1 (MQ=255)
                                                 tgtgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgatg                                                                                         >  7:240664/1‑149 (MQ=255)
                                                   tgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAgg                                                                                                            >  2:236718/1‑128 (MQ=255)
                                                   tgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAgg                                                                                                            <  1:236718/128‑1 (MQ=255)
                                                         cTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTg                                                                                  <  8:57419/148‑1 (MQ=255)
                                                                ccAGCCTCTCTTCCACTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCt                                                                           <  8:276911/148‑1 (MQ=255)
                                                                 cAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTg                                                                          >  5:110000/1‑148 (MQ=255)
                                                                  aGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             >  1:141537/1‑112 (MQ=255)
                                                                  aGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                                                                                             <  2:141537/112‑1 (MQ=255)
                                                                    ccTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGgaag                                                                      <  5:71903/149‑1 (MQ=255)
                                                                            ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAATTTTATAATGTAGGGCATCCTTACCGATCTGTGGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt                                                                <  1:214426/147‑1 (MQ=255)
                                                                            ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt                                                                <  8:358876/147‑1 (MQ=255)
                                                                            ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACa                                                                 <  4:164232/146‑1 (MQ=255)
                                                                            ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt                                                                <  2:284340/147‑1 (MQ=255)
                                                                             cccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCATCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTc                                                              >  7:11979/1‑148 (MQ=255)
                                                                              ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt                                                                                   >  8:331704/1‑126 (MQ=255)
                                                                              ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt                                                                                   <  7:331704/126‑1 (MQ=255)
                                                                              ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCg                                                             >  7:362461/1‑148 (MQ=255)
                                                                              ccTGAAGGTTTCGAGCAAGCTTCCGCGAGTATGGGCCGCTTCGTCGTCAAATTTATCCTGTAGGGCCTCCTTACCGCTCTGGCGTATCCCGTCGTCGTAGGTATTCGCCACCTGAGGATGGATTGTGGCTATATGGAAGCAACATTCg                                                             >  1:321767/1‑148 (MQ=255)
                                                                                  aaGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGc                                                         <  5:170048/148‑1 (MQ=255)
                                                                                     gTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCggg                                                      >  6:149570/1‑148 (MQ=255)
                                                                                      tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCggg                                                      <  4:248464/147‑1 (MQ=255)
                                                                                      tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGt                                                     >  8:17989/1‑148 (MQ=255)
                                                                                      tttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTa                                                    <  1:115489/149‑1 (MQ=255)
                                                                                           aGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGcc                                                                                                    <  6:145732/96‑1 (MQ=255)
                                                                                           aGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGcc                                                                                                    >  5:145732/1‑96 (MQ=255)
                                                                                            gCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCt                                              >  8:232286/1‑149 (MQ=255)
                                                                                                gCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGc                                          <  6:110000/149‑1 (MQ=255)
                                                                                                  ttCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGcc                                         >  1:311705/1‑148 (MQ=255)
                                                                                                      gcgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAAt                                    >  1:107396/1‑149 (MQ=255)
                                                                                                       cgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaa                                       >  1:117580/1‑145 (MQ=255)
                                                                                                       cgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaa                                       <  2:117580/145‑1 (MQ=255)
                                                                                                          ttATGGGCAGCTTCTTCGTCAAATTTATCATGTAGCGCATCCAAACCGCTCTCGCGTATCACGTCGTCGTAGCTATTCGCCACATGATGATGGATTTTGGCTATCTGGAACAAACATTCGAAGCGCGTAAACGCTCCGCCAAAAtcta                                 >  1:213728/1‑147 (MQ=255)
                                                                                                             tGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCttt                             <  8:362461/149‑1 (MQ=255)
                                                                                                             tGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCtt                              <  8:240664/148‑1 (MQ=255)
                                                                                                                   gCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGttatt                       <  5:149570/149‑1 (MQ=255)
                                                                                                                    cttcttCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGttatt                       >  7:148286/1‑148 (MQ=255)
                                                                                                                     ttcttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAc                     >  8:75385/1‑149 (MQ=255)
                                                                                                                       cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGTAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAc                     <  2:232712/147‑1 (MQ=255)
                                                                                                                       cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTg                   >  8:20397/1‑149 (MQ=255)
                                                                                                                        ttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcg                                                                                                                   <  3:338468/52‑1 (MQ=255)
                                                                                                                        ttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcg                                                                                                                   >  4:338468/1‑52 (MQ=255)
                                                                                                                        ttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGt                  >  1:216548/1‑149 (MQ=255)
                                                                                                                           gTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGt               >  4:226232/1‑149 (MQ=255)
                                                                                                                           gTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGt               >  7:377591/1‑149 (MQ=255)
                                                                                                                            tCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTg              >  3:358486/1‑149 (MQ=255)
                                                                                                                            tCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTAGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTg              >  5:1036/1‑149 (MQ=255)
                                                                                                                             cAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGc             >  2:17774/1‑149 (MQ=255)
                                                                                                                                aTTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCttt          >  7:252336/1‑149 (MQ=255)
                                                                                                                                 tttATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTc         <  3:311373/149‑1 (MQ=255)
                                                                                                                                   tATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCa        <  2:22786/148‑1 (MQ=255)
                                                                                                                                   tATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCa        <  8:377591/148‑1 (MQ=255)
                                                                                                                                       aTGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTACTTTGTTATTACTGTCGTGCTTTCACTtct   >  2:357431/1‑149 (MQ=255)
                                                                                                                                        tgtaGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTtctc  >  7:221718/1‑149 (MQ=255)
                                                                                                                                        tgtaGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTtctc  >  2:382040/1‑149 (MQ=255)
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CTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTC  >  NC_000913/755261‑755545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: